PREMIUM Sanger SERVICES

Let GENEWIZ take over project management for your next project! Full-service Sanger sequencing options allow researchers to hand off an entire sequencing project from primer design to variance analysis and report generation. Template discovery service options include primer walking, sequence confirmation, and colony screening.


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PRIMER WALKING

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Is your freezer full of unmapped or mystery constructs? Do your transformations grow on the wrong antibiotic resistance plate? GENEWIZ Primer Walking service is designed for situations when you need to determine the sequence of a completely unknown plasmid or PCR product.

Our Service Includes

  • Primer design and synthesis 
  • Plasmid preparation or RCA (if needed) 
  • Single-stranded or double-stranded coverage per base pair as dictated by project requirements 
  • Manual editing based on sequence chromatogram 
  • Sequence data assembly for consensus sequence 
  • Consensus report

Upon Order Submission

Please send to GENEWIZ:

  • Template, in tubes or plates
  • Known sequence starting point, such as antibiotic resistance, M13 promoter, etc.
  • Requested number of base pairs sequenced
  • Single-stranded or double-stranded coverage requirements

Project Deliverables

  • Standard turnaround time starting at 1 – 2 kb/week
  • Consensus sequence, in .seq or .fasta format
  • All raw sequencing data, in .ab1 and .seq formats
  • Any size plasmid prep, upon request

SEQUENCE CONFIRMATION SERVICE

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Do your digestions show incorrect banding patterns? Does your construct fail to sequence or amplify with the anticipated primer set? GENEWIZ Sequence Confirmation service is designed for situations when you need to verify the identity of your construct with single- or double-stranded certainty.

Our Service Includes

  • Primer design and synthesis  
  • Plasmid preparation or RCA (if needed)
  • Single-stranded or double-stranded coverage per base pairs as dictated by project requirements 
  • Manual editing based on sequence chromatogram 
  • Sequence data assembly for consensus sequence 
  • Consensus report

Upon Order Submission

Please send to GENEWIZ:

  • Template, in tubes or plates
  • Reference sequence, in .fasta, .txt. or .seq format
  • Requested number of base pairs sequenced
  • Single-stranded or double-stranded coverage requirements


Project Deliverables

  • Standard turnaround time starting at 5-7 business days
  • Consensus sequence, in .seq or .fasta format
  • Variance report, if applicable
  • All raw sequencing data, in .ab1 and .seq formats
  • Any size plasmid prep, upon request

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.



Email | Phone (1-877-GENEWIZ (436-3949), Ext. 2) | Live Chat