WHOLE PLASMID SEQUENCING: PLASMID-EZ

Plasmid-EZ is an end-to-end solution that enables cost-effective, rapid, and high-throughput sequencing of whole plasmids. With conventional methods, only important regions of plasmids are sequenced which can fail to detect unwanted changes in other areas during replication and leave you to piece together an entire plasmid map. Using long-read sequencing, Plasmid-EZ provides complete verification of plasmid integrity and fidelity with results delivered in as fast as 1 business day upon receipt in our processing lab.

Now connected to our expansive dropbox network, Plasmid-EZ offers unparalleled convenience for sample submission. Simply order directly through our customer portal and deposit your samples at one of our convenient dropboxes.

PLASMID CONSTRUCTION WORKFLOW WITH PLASMID-EZ

COMPLETE PLASMID SEQUENCING WORKFLOW

Technology Comparison: Whole Plasmid Sequencing

Metric	Sanger Sequencing	Plasmid-EZ	Primer Walking	NGS
Starting Material	Plasmid DNA	Plasmid DNA	Plasmid DNA	Plasmid DNA
Region Sequenced	Targeted Regions	Full-Length Plasmid	Long Targeted Regions	Full-Length Plasmid
Cost	$	$$	$$$	$$$
Read Quality	+++	++	+++	++++
Read Length	++	++++	++	+/++++
Automatic Assembly
Gene Annotations
Other Requirements	Must provide primer		Must provide reference

Deliverables

• FASTQ files
• Read-length and quality report
• Consensus plasmid assembly
• Plasmid annotation files

Confirm your plasmid sequences with Azenta, a worldwide leader in sequencing since 1999

Features & Benefits

Quick Turnaround Times

In as fast as 1 business day upon receipt in our processing lab for rapid plasmid validation

Easily Submit Samples

At any one of our convenient dropbox locations

Interactive Data Report

Provides an easy way to understand sequencing results

High-throughput, plasmid-to-data solution for rapid and interactive construct validation

Same price across all plasmids, regardless of size and complexity

Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates

Chosen provider for all top 30 biopharma

Technical Resources

NGS Platforms

GENEWIZ is a certified service provider for Illumina and other cutting-edge NGS platforms. For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.