WHOLE PLASMID SEQUENCING: PLASMID-EZ
Whole plasmid sequencing (Plasmid-EZ) is our end-to-end solution for cost-effective, rapid, and high-throughput sequencing of entire plasmids. Unlike conventional methods that only sequence selected regions, Plasmid-EZ verifies the integrity and fidelity of whole plasmids using long-read sequencing. This ensures the detection of unwanted changes, providing a complete plasmid map and preventing potential replication issues.
Now connected to our expansive dropbox network, Plasmid-EZ offers unparalleled convenience for sample submission – easily order directly through our customer portal and deposit your samples at one of our convenient dropboxes. Upon receipt in our processing lab, you will receive results in as fast as one business day.
What is whole plasmid sequencing?
Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).
Whole plasmid sequencing provides detailed insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies. By sequencing the whole plasmid, genomic data can be used to further the understanding of microbial diversity, evolution, and mechanisms underlying biological processes.
How is whole plasmid sequencing done?
Whole plasmid sequencing is carried out by isolating the plasmid DNA (pDNA), splicing the pDNA into smaller fragments, sequencing said fragments, then assembling the plasmid sequence data to reconstruct the full plasmid genome. Bioinformatics tools are then used to analyze the resulting sequencing data.
Direct Colony to Whole Plasmid Sequencing
Simplify your research workflow and enhance your productivity with our advanced whole plasmid sequencing service (Plasmid-EZ), now accepting bacterial samples as starting material. With our experts performing plasmid preps and sequencing, we’ve streamlined our process to efficiently deliver results in as fast as 2 business days. Experience the ease and excellence of our sequencing solutions today.
PLASMID CONSTRUCTION WORKFLOW WITH PLASMID-EZ
COMPLETE PLASMID SEQUENCING WORKFLOW
Technology Comparison: Whole Plasmid Sequencing
| Metric | Sanger Sequencing | Plasmid-EZ | Primer Walking | NGS |
| Starting Material | Plasmid DNA | Plasmid DNA or Bacteria* | Plasmid DNA | Plasmid DNA |
| Region Sequenced | Targeted Regions | Full-Length Plasmid | Long Targeted Regions | Full-Length Plasmid |
| Cost | $ | $$ | $$$ | $$$ |
| Read Quality | +++ | ++ | +++ | ++++ |
| Read Length | ++ | ++++ | ++ | +/++++ |
| Automatic Assembly | ||||
| Gene Annotations | ||||
| Other Requirements | Must provide primer | Must provide reference |
*We accept bacterial samples as colonies, liquid culture and glycerol stock
Features & Benefits
Rapid Turnaround Times
Same-day results in designated areas or in as fast as 1 business day
Easily Submit Samples
At any one of our convenient dropbox locations
Interactive Data Report
Provides an easy way to understand sequencing results
High-throughput, plasmid-to-data solution for rapid and interactive construct validation
Same price across all plasmids, regardless of size and complexity
Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates
Flexible sample types accepted – plasmid DNA, bacterial colonies, liquid culture, and glycerol stock
Technical Resources
Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.
Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.
NGS Platforms
GENEWIZ is a service provider of cutting-edge NGS platforms. For information on our NGS platforms and recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ from Azenta does not guarantee data output or quality for sequencing-only projects.
