Whole Plasmid Sequencing
Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA). It enables insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies.
GENEWIZ Plasmid-EZ sequences the whole plasmid, providing genomics data to further understand microbial diversity, evolution, and the mechanisms underlying biological processes. We also offer amplicon sequencing using our PCR-EZ and Amplicon-EZ platforms.
Whole Plasmid Sequencing Services
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Plasmid-EZ
Plasmid-EZ is our end-to-end solution for cost-effective, rapid, and high-throughput sequencing of entire plasmids.
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Direct Colony to Whole Plasmid Sequencing
Simplify your research workflow and enhance your productivity with our advanced whole plasmid sequencing service (Plasmid-EZ), now accepting bacterial samples as starting material.
Amplicon Sequencing Services
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PCR-EZ
PCR-EZ product sequencing is a long-read NGS-based PCR sequencing service that utilizes Oxford Nanopore Technologies® (ONT)
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Amplicon-EZ
Amplicon-EZ is a streamlined version of next generation sequencing to analyze heterogeneous PCR products.
Weekly Drawing
Tubesday
Submit sequencing orders to win GENEWIZ swag
Enter every Tuesday through March.
Service Comparison: Complete Plasmid Sequencing & Amplicon Sequencing
| GENEWIZ Service | Starting Material | Technology | Read Quality | Read Length | Reads / Read Depth | Automatic Assembly | Best For |
|---|---|---|---|---|---|---|---|
| Sanger Sequencing | Purified or unpurified PCR product | Sanger | Higher Quality | <1000 bp | 1–4 | — |
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| Amplicon-EZ | Purified PCR product | Illumina® Short-read NGS | Best Quality | 150–500 bp | 50,000 | Included |
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| PCR-EZ | Purified or unpurified PCR product | Oxford Nanopore® Long-read NGS | Good Quality | <25 kb | 1,000 | Included |
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| Long-Read Amplicon Sequencing | Purified PCR product | PacBio® Long-read NGS | Best Quality | <25 kb | 100,000 | Included |
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*If you are sequencing larger than 25 kb amplicons, please submit an inquiry for a technical consultation.
What is whole plasmid sequencing?
Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).
Whole plasmid sequencing provides detailed insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies. By sequencing the whole plasmid, genomic data can be used to further the understanding of microbial diversity, evolution, and mechanisms underlying biological processes.
How is whole plasmid sequencing done?
Whole plasmid sequencing is carried out by isolating the plasmid DNA (pDNA), splicing the pDNA into smaller fragments, sequencing said fragments, then assembling the plasmid sequence data to reconstruct the full plasmid genome. Bioinformatics tools are then used to analyze the resulting sequencing data.
Features & Benefits
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Rapid Turnaround Times
Same-day results in designated areas or as fast as 8:00AM next day
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Easily Submit Samples
At any one of our convenient dropbox locations
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Interactive Data Report
Provides an easy way to understand sequencing results
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High-throughput, plasmid-to-data solution for rapid and interactive construct validation directly from bacteria
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Same price across all plasmids, regardless of size and complexity
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Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates
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Flexible sample types accepted – bacterial colonies, liquid culture, and glycerol stock
*Results available by 8 AM the next morning for customers located near one of our 6 overnight sequencing labs. For customers who ship samples to our New Jersey facility, results will be available by the end of the day that the samples are received.
Technical Resources
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Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis.
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Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.