Tech Note │ An Efficient and High-Fidelity Approach to AAV Plasmid Preparation

Preserving the integrity of inverted terminal repeat (ITR) sequences in adeno-associated virus (AAV) plasmids is important to produce recombinant AAV (rAAV) vectors. The proprietary AAV plasmid preparation service from GENEWIZ delivers superior quality for midi to giga-scale AAV vectors, while maintaining the integrity of ITR regions. Our new process includes pre- and post-preparation quality controlled (QC) steps that leverage GENEWIZ AAV-ITR Sanger sequencing capabilities for full sequence verification and ensures delivery of intact ITR clones for your research.

Tech Note | AAV-ITR Sequencing

GENEWIZ has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids.

Tech Note │ Multiplexing microbes on the PacBio Sequel: Feasibility, benefits, and recent developments

This study proves the feasibility of multiplexing and highlights the key advantages of the PacBio Sequel platform as compared to its predecessor, the RSII.

Tech Note | Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal

There are multiple factors to consider when selecting which RNA-Seq technique is the right approach for your project. In this eBook, discover the what, why, and how of RNA-Seq, the most common types of assays and platforms, and uncover insights into how to select the best method to achieve optimal results for your research.

Tech Note │ An Efficient and Reliable Approach to AAV Packaging

Previous reports have shown that ITRs are necessary to produce effective viral particles, but their structure is problematic for conventional sequencing, synthesis, and cloning workflows. Here, we study the effect of partial and full ITR deletions on AAV packaging and show how the proprietary methods developed by GENEWIZ can successfully detect and repair these mutations.

Tech Note │ Enhanced Gene Detection with GEM-X Single-Cell Sequencing

This tech note highlights how 10x Genomics’ GEM-X chemistry enhances single-cell RNA sequencing by improving cell recovery, gene detection, and immune profiling, all while reducing costs per cell. Unlock these insights and optimize your single-cell analysis.

Tech Note │ High-Resolution Spatial Profiling and Biomarker Discovery by Visium HD

In this tech note we explore how the spatial transcriptomics platform, Visium HD®, bridges the gap between histology and gene expression profiling, offering precise biomarker discovery and cellular mapping within complex tissues to support research across oncology, immunology, and developmental biology.​

Tech Note │ Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins

Plasmid DNA (pDNA) is crucial in the fields of gene therapy, vaccine development, and molecular cloning. However, achieving high yields of high-quality pDNA while minimizing endotoxin contamination can be challenging. This tech note will help you significantly increase pDNA yield and dramatically reduce endotoxin levels through advanced protocol improvements, enhancing the efficiency and success of your experiments.​

Tech Note │ High-Fidelity Production of In Vitro Transcription Plasmids with Long Poly(A) Sequences

Previous reports have shown that ITRs are necessary to produce effective viral particles, but their structure is problematic for conventional sequencing, synthesis, and cloning workflows. Here, we study the effect of partial and full ITR deletions on AAV packaging and show how the proprietary methods developed by GENEWIZ can successfully detect and repair these mutations.

Tech Note │ High-Plex Exploratory Biomarker Discovery to Support Proteomics and Multiomics Research

In this tech note, discover the science behind the Olink® Proximity Extension Assay (PEA) and how its optimal approach makes it easier to incorporate protein biomarker discovery into your preclinical and clinical research.

Tech Note │ Proteomic Biomarker Discovery to Support Clinical Trials

In this tech note, discover the science behind the Olink® Proximity Extension Assay (PEA) and how its optimal approach makes it easier to incorporate protein biomarker discovery into your preclinical and clinical research.

Tech Note | Full-Length RNA-Seq: A Novel Method to Assess Sequence Integrity for RNA Therapeutics

Strict quality control is required to maintain the integrity of manufactured products for RNA therapies, but current assays often present limitations. Learn how the GENEWIZ novel full-length RNA-Seq approach allows you to preserve the entire length of and effectively sequence the poly(A) tails of your mRNA products.

Tech Note | Isoform Sequencing on the PacBio Sequel® – Maximizing Output and Accuracy

Contiguous mRNA full-length sequencing (Iso-Seq) greatly simplifies genome annotation efforts and revolutionizes the discovery of novel RNA isoforms. This Tech Note discusses the advantages of the latest technologies combined with GENEWIZ optimized workflow, and how this increases output and accuracy.

Tech Note | Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening

In this tech note, we introduce high-throughput gene expression (HT-GEx) screening, an optimized assay developed by GENEWIZ that utilizes cell lysate instead of purified RNA to perform rapid, unbiased phenotypic profiling at a reduced cost – an ideal approach for drug discovery, compound treatment phenotyping, CRISPR treatment phenotyping, and cell response screening.

Tech Note | Synthesis of High-Quality Gene Fragments

Gene fragments are linear, double-stranded DNA sequences used for cloning into vectors and other downstream applications. Cloning can be done in-house or outsourced, but success depends on fragment quality—low fidelity can lead to lengthy screening. In this technical note, GENEWIZ from Azenta shows how high-quality, accurate gene fragments enable faster, more reliable molecular biology workflows.

Tech Note | mRNA Production via Integrated Gene Synthesis and In Vitro Transcription

In vitro transcription (IVT) is a powerful technique to generate messenger RNA (mRNA) for a wide array of research and therapeutic applications. Transfection of mRNA enables very high protein expression, comparable to viral systems, with rapid onset. Read our tech note to learn how IVT, coupled with gene synthesis, can be used to synthesize mRNA of virtually any sequence quickly and efficiently.

Tech Note | Enhancing Protein Expression by Leveraging Codon Optimization

Codon preference is among the most critical parameters affecting the expression level of foreign genes. This tech note will discuss two practical case studies, comparing wild-type gene performance to our codon-optimized sequences.