Whole Plasmid Sequencing
Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA). It enables insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies.
Whole plasmid sequencing is carried out by isolating the plasmid DNA (pDNA), splicing the pDNA into smaller fragments, sequencing said fragments, then assembling the plasmid sequence data to reconstruct the full plasmid genome. Bioinformatics tools are then used to analyze the resulting sequencing data.
GENEWIZ Plasmid-EZ sequences the whole plasmid, providing genomics data to further understand microbial diversity, evolution, and the mechanisms underlying biological processes. We also offer amplicon sequencing using our PCR-EZ and Amplicon-EZ platforms.
Whole Plasmid Sequencing Services
Amplicon Sequencing Services
Service Comparison: Complete Plasmid Sequencing & Amplicon Sequencing
| GENEWIZ Service | Starting Material | Technology | Read Quality | Read Length | Reads / Read Depth | Automatic Assembly | Best For |
|---|---|---|---|---|---|---|---|
| Sanger Sequencing |
Purified or unpurified PCR product | Sanger | Higher Quality | <1000 bp | 1 – 4 | – |
|
| Amplicon-EZ |
Purified PCR product | Illumina® Short-read NGS | Best Quality | 150-500 bp | 50,000 Custom options available > |
Included |
|
| PCR-EZ |
Purified or unpurified PCR product | Oxford Nanopore® Long-read NGS | Good Quality | <25 kb* | 1,000 | Included |
|
| Long-Read Amplicon Sequencing |
Purified PCR product | PacBio® Long-read NGS | Best Quality | <25 kb* | 100,000 | Included |
|
*If you are sequencing larger than 25 kb amplicons, please submit an inquiry for a technical consultation.
What is whole plasmid sequencing?
Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).
Whole plasmid sequencing provides detailed insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies. By sequencing the whole plasmid, genomic data can be used to further the understanding of microbial diversity, evolution, and mechanisms underlying biological processes.
How is whole plasmid sequencing done?
Whole plasmid sequencing is carried out by isolating the plasmid DNA (pDNA), splicing the pDNA into smaller fragments, sequencing said fragments, then assembling the plasmid sequence data to reconstruct the full plasmid genome. Bioinformatics tools are then used to analyze the resulting sequencing data.
Features & Benefits
Rapid Turnaround Times
Same-day results in designated areas or as fast as 8:00AM next day
Easily Submit Samples
At any one of our convenient dropbox locations
Interactive Data Report
Provides an easy way to understand sequencing results
High-throughput, plasmid-to-data solution for rapid and interactive construct validation
Same price across all plasmids, regardless of size and complexity
Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates
Flexible sample types accepted – plasmid DNA, bacterial colonies, liquid culture, and glycerol stock
Technical Resources
Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.
Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.