Plasmid-EZ Plasmid Sequencing Service
GENEWIZ Plasmid-EZ Sequencing service is our end-to-end solution for cost-effective, rapid, and high-throughput sequencing of entire plasmids. Unlike conventional methods that only sequence selected regions, Plasmid-EZ verifies the integrity and fidelity of whole plasmids using long-read sequencing. This ensures the detection of unwanted changes, providing a complete plasmid map and preventing potential replication issues.
For the fastest turnaround times, easily submit your sample in a GENEWIZ dropbox. Order directly through our customer portal and then deposit your samples at one of more than 1800+ convenient dropboxes. Upon receipt in our processing lab, you will receive results in as fast as one business day.
Plasmid Construction Workflow with Plasmid-EZ
Complete Plasmid Sequencing Workflow
Why Researchers Choose GENEWIZ
“I’m highly satisfied with the service each time I have used it! Results are fast, thorough, and easy to interpret. 5/5 stars from me!”
“I’ve been using your whole plasmid sequencing service directly from glycerol stock, and it’s been a great experience—fast turnaround and high-quality results.”
“The results look great! I was able to identify multiple error-free clones for this construct and selected one for midi-prep scale-up. The improved process and TAT are great! I didn’t need to pick colonies, which saved me a ton of time.”
“I have been using the GENEWIZ Plasmid-EZ (whole plasmid sequencing) service with liquid cultures, and I love it so far!”
Technology Comparison: Colony to Whole Plasmid Sequencing
| GENEWIZ Service | Starting Material | Region Sequenced | Cost | Read Quality | Read Length | Automatic Assembly | Gene Annotations | Other Requirements |
|---|---|---|---|---|---|---|---|---|
Plasmid-EZ |
Plasmid DNA or Bacteria* | Full-Length Plasmid | $$ | Good Quality | Best Quality | Included | Included | — |
Sanger Sequencing |
Bacteria* | Targeted Regions | $ | Higher Quality | Good Quality | — | — | Must provide primer |
Next Generation Sequencing |
Plasmid DNA | Full-Length Plasmid | $$$ | Best Quality | Best Quality | Included | Included | — |
*We accept bacterial samples as colonies, liquid culture and glycerol stock
The GENEWIZ Difference
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Rapid Turnaround Times
Same-day results in designated areas or as fast as 8:00AM next day
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Easily Submit Samples
At one of our 1,800+ conveniently located dropboxes
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Interactive Data Report
Provides an easy way to understand sequencing results
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High-throughput, plasmid-to-data solution for rapid and interactive construct validation directly from bacteria
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Same price across all plasmids, regardless of size and complexity
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Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates
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Flexible sample types accepted – bacterial colonies, liquid culture, and glycerol stock
*Results available by 8 AM the next morning for customers located near one of our 6 overnight sequencing labs. For customers who ship samples to our New Jersey facility, results will be available by the end of the day that the samples are received.
Deliverables
- FastQ files
- Read-length and quality report
- Consensus plasmid assembly
- Plasmid annotation files
- Assembled sequence in
.ab1format - Variant calling result in
VCFfile
Confirm your plasmid sequences with GENEWIZ, a worldwide leader in sequencing since 1999
Technical Resources
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Blog | From Colonies to Confirmed Plasmids: A Workflow Many of Us Know Too Well
Discusses challenges researchers face during plasmid cloning and validation workflows, particularly after bacterial colony screening following cloning experiments. It highlights how whole plasmid sequencing using Nanopore technology can provide faster, more comprehensive construct confirmation to help accelerate downstream research.
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Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis.
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Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.
NGS Platforms
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
