Plasmid-EZ Sequencing

Plasmid-EZ Sequencing is our end-to-end solution for cost-effective, rapid, and high-throughput sequencing of entire plasmids. Unlike conventional methods that only sequence selected regions, Plasmid-EZ verifies the integrity and fidelity of whole plasmids using long-read sequencing. This ensures the detection of unwanted changes, providing a complete plasmid map and preventing potential replication issues.

Now connected to our expansive dropbox network, Plasmid-EZ offers unparalleled convenience for sample submission – easily order directly through our customer portal and deposit your samples at one of our convenient dropboxes. Upon receipt in our processing lab, you will receive results in as fast as one business day.

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Plasmid Construction Workflow with Plasmid-EZ​

Complete plasmid construction workflow.

Complete Plasmid Sequencing Workflow

Complete plasmid-ez sequencing workflow.

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Technology Comparison: Colony to Whole Plasmid Sequencing

Comparison of GENEWIZ services by starting material, region sequenced, cost, read quality, read length, assembly, annotations, and requirements.
GENEWIZ Service Starting Material Region Sequenced Cost Read Quality Read Length Automatic Assembly Gene Annotations Other Requirements

Plasmid-EZ

 Plasmid DNA or Bacteria* Full-Length Plasmid $$ Good Quality Best Quality Included Included —

Sanger Sequencing

 Bacteria* Targeted Regions $ Higher Quality Good Quality — — Must provide primer

Primer Walking

 Plasmid DNA Long Targeted Regions $$$ Higher Quality Good Quality Included — Must provide reference

Next Generation Sequencing

 Plasmid DNA Full-Length Plasmid $$$ Best Quality Best Quality Included Included —

*We accept bacterial samples as colonies, liquid culture and glycerol stock

Features & Benefits

  • Rapid Turnaround Times

    Same-day results in designated areas or as fast as 8:00AM next day​​

  • Easily Submit Samples

    At any one of our convenient dropbox locations

  • Interactive Data Report

    Provides an easy way to understand sequencing results

  • High-throughput, plasmid-to-data solution for rapid and interactive construct validation directly from bacteria
  • Same price across all plasmids, regardless of size and complexity
  • Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates
  • Flexible sample types accepted – bacterial colonies, liquid culture, and glycerol stock

*Results available by 8 AM the next morning for customers located near one of our 6 overnight sequencing labs. For customers who ship samples to our New Jersey facility, results will be available by the end of the day that the samples are received.​

Technical Resources

  • Thumbnail for Webinar titled From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing

    Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing

    While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis.

    Watch Webinar
  • Thumbnail for Tech Note titled Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins

    Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins

    Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.

    Read Tech Note

Deliverables

  • Assembled sequence in .ab1 format
  • Variant calling result in VCF file

NGS Platforms

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.