Plasmid-EZ Sequencing

Plasmid-EZ Sequencing is our end-to-end solution for cost-effective, rapid, and high-throughput sequencing of entire plasmids. Unlike conventional methods that only sequence selected regions, Plasmid-EZ verifies the integrity and fidelity of whole plasmids using long-read sequencing. This ensures the detection of unwanted changes, providing a complete plasmid map and preventing potential replication issues.

Now connected to our expansive dropbox network, Plasmid-EZ offers unparalleled convenience for sample submission – easily order directly through our customer portal and deposit your samples at one of our convenient dropboxes. Upon receipt in our processing lab, you will receive results in as fast as one business day.

PLASMID CONSTRUCTION WORKFLOW WITH PLASMID-EZ

COMPLETE PLASMID SEQUENCING WORKFLOW

Technology Comparison: Plasmid-EZ Sequencing

GENEWIZ Service	Starting Material	Region Sequenced	Cost	Read Quality	Read Length	Automatic Assembly	Gene Annotations	Other Requirements
Plasmid-EZ	Plasmid DNA or Bacteria*	Full-Length Plasmid	$$	Good Quality	Best Quality	Included	Included	–
Sanger Sequencing	Plasmid DNA	Targeted Regions	$	Higher Quality	Good Quality	–	–	Must provide primer
Primer Walking	Plasmid DNA	Long Targeted Regions	$$$	Higher Quality	Good Quality	Included	–	Must provide reference
Next Generation Sequencing	Plasmid DNA	Full-Length Plasmid	$$$	Best Quality	Best Quality	Included	Included	–

*If you are sequencing larger than 25 kb amplicons, please submit an inquiry for a technical consultation.

Features & Benefits

Rapid Turnaround Times

Same-day results in designated areas or as fast as 8:00AM next day

Easily Submit Samples

At any one of our convenient dropbox locations

Interactive Data Report

Provides an easy way to understand sequencing results

High-throughput, plasmid-to-data solution for rapid and interactive construct validation

Same price across all plasmids, regardless of size and complexity

Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates

Flexible sample types accepted – plasmid DNA, bacterial colonies, liquid culture, and glycerol stock

Technical Resources

Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing

While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.

Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins

Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.

Deliverables

Assembled sequence in .ab1 format
Variant calling result in VCF file

NGS Platforms

GENEWIZ is a service provider of cutting-edge NGS platforms. For information on our NGS platforms and recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ from Azenta does not guarantee data output or quality for sequencing-only projects.