Colony to Whole Plasmid Sequencing: Colony-EZ
Colony to whole plasmid sequencing (Colony-EZ) is a fast, cost-effective and fully streamlined solution for sequencing entire plasmids directly from bacterial samples, no plasmid prep required. Skip the time-consuming extraction steps and submit your colonies, glycerol stocks or liquid cultures as-is at your nearest GENEWIZ Dropbox or via courier.
Our colony to whole plasmid sequencing experts isolate and sequence your plasmids for you, delivering high-quality whole plasmid sequencing results by 8 AM the next morning*, so you can accelerate your workflow and stay focused on advancing your research.
What is colony to whole plasmid sequencing?
Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).
Colony to Whole Plasmid Sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA), directly from bacteria cells. Whole plasmid sequencing provides detailed insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies. By sequencing the whole plasmid, genomic data can be used to further the understanding of microbial diversity, evolution, and mechanisms underlying biological processes.
How does the colony to Whole Plasmid Sequencing work?
The colony to whole plasmid sequencing workflow starts with a single picked bacterial colony. The plasmid DNA is lysed, barcoded, and prepared for long-read sequencing without a mini-prep step. Sequencing then generates full-length plasmid reads, which are assembled and analyzed to confirm the entire plasmid sequence, including insert identity, orientation, junctions, and vector backbone integrity. Results are delivered rapidly with high accuracy and minimal sample preparation.
Plasmid Construction Workflow with Colony-EZ
Already prepped your plasmids?
Simply send your purified samples directly to our whole plasmid sequencing service (Plasmid-EZ) for fast, high-quality whole plasmid sequencing. Submit samples at the nearest GENEWIZ Dropbox or via courier, and our team will handle the rest, delivering comprehensive sequencing results by 8 AM the next morning* to keep your research moving at full speed.
Complete Colony to Plasmid Sequencing Workflow
Customer Testimonials for Colony to Whole Plasmid Sequencing
“The results look great! I was able to identify multiple error-free clones for this construct and selected one for midi-prep scale-up. The improved process and TAT are great! I didn’t need to pick colonies which saved me a ton of time.”
“I am very pleased with the TAT and results! As a company, we value minimizing any wait time since most experiments are gated by plasmid prep. I appreciate the consistency of the results, the ability to do LTR colony sequencing, and at a great price!”
Technology Comparison: Colony to Whole Plasmid Sequencing
| GENEWIZ Service | Starting Material | Region Sequenced | Cost | Read Quality | Read Length | Automatic Assembly | Gene Annotations | Other Requirements |
|---|---|---|---|---|---|---|---|---|
Colony-EZ |
Bacteria* | Full-Length Plasmid | $$ | Good Quality | Best Quality | Included | Included | — |
Sanger Sequencing |
Bacteria* | Targeted Regions | $ | Higher Quality | Good Quality | — | — | Must provide primer |
Primer Walking |
Plasmid DNA | Long Targeted Regions | $$$ | Higher Quality | Good Quality | Included | — | Must provide reference |
Next Generation Sequencing |
Plasmid DNA | Full-Length Plasmid | $$$ | Best Quality | Best Quality | Included | Included | — |
*We accept bacterial samples as colonies, liquid culture and glycerol stock
Deliverables
- FastQ files
- Read-length and quality report
- Consensus plasmid assembly
- Plasmid annotation files
- Assembled sequence in
.ab1format - Variant calling result in
VCFfile
Confirm your plasmid sequences with GENEWIZ, a worldwide leader in sequencing since 1999
Features & Benefits
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Rapid Turnaround Times
Receive your sequencing results by 8 AM the next morning*
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Easily Submit Samples
At any one of our convenient dropbox locations
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Interactive Data Report
Provides an easy way to understand sequencing results
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High-throughput, plasmid-to-data solution for rapid and interactive construct validation directly from bacteria
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Same price across all plasmids, regardless of size and complexity
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Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates
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Flexible sample types accepted – bacterial colonies, liquid culture, and glycerol stock
*Results available by 8 AM the next morning for customers located near one of our 6 overnight sequencing labs. For customers who ship samples to our New Jersey facility, results will be available by the end of the day that the samples are received.
Technical Resources
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Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis.
NGS Platforms
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
