Whole Plasmid and Amplicon Sequencing Sample Submission Guidelines

Whole Plasmid Sequencing

Order Today
  1. GENEWIZ Drop Box or Fridge – Place your samples and a copy of your order form in a GENEWIZ drop box or fridge before the cut-off time. Call 1-877-436-3949 for your nearest GENEWIZ drop-off location.
  2. GENEWIZ Courier Service – Request for courier pickup if available at your location. Please call 1-877-436-3949 to find out if a GENEWIZ courier picks up at your site. For San Diego customers within the pickup zone, specify the pickup location when placing your order. Note that GENEWIZ couriers pick up room temperature samples only.
  3. If a drop box or courier is not available, and for International shipments, ship your samples via overnight delivery service (FedEx if possible) to us at the following location:

    GENEWIZ from Azenta
    115 Corporate Blvd.
    South Plainfield, NJ 07080
    USA
    908-222-0711
Plasmid-EZ (Whole Plasmid Sequencing)
Sample Type Quantity Quality Shipping Temperature
Plasmid DNA*
Water, EB, Low TE buffer (0.1mM EDTA)		 Minimum Amount:
Quantity: ≥500 ng
Volume: ≥10 µL
Concentration: 50 ng/µL Normalized		 A260/A280 = 1.8-2.0 Ambient or blue ice
Bacteria
  • Colonies on agar
  • Liquid culture
  • Glycerol stock
  • Circle and number colonies or leave blank for random pick.
  • 100ul of overnight-grown liquid culture (8 to 16 hours)
  • 100ul of glycerol per clone
  • Individual colonies should be widely spaced on agar plate
  • For liquid culture and glycerol stock, sample should be visibly cloudy
  • Agar and liquid culture – Ambient or blue ice
  • Glycerol stock – dry ice
Colony-EZ (Whole Plasmid Sequencing from Bacteria)
Sample Type Quantity Quality Shipping Temperature
Bacterial Colonies
Bacteria* BSL1 specimens only		 1 colony per well submitted in a 96-well agar stab plate 1 well-grown colony per well (incubated for at least 8h) Ambient or blue ice
Liquid Culture
Bacteria* BSL1 specimens only		 10-50 µL of an overnight culture (8-16h) submitted in 96-well plates (we will inoculate an overnight culture before proceeding with Plasmid-Prep)

500 µL of an overnight culture (8-16h) submitted in 96-well plates (we will directly proceed with Plasmid Prep)		 Samples should be visibly cloudy Ambient or blue ice
Glycerol Stock
Bacteria* BSL1 specimens only		 100ul of glycerol stock Samples should be visibly cloudy Dry ice
Colony-EZ Plus (Whole Plasmid Sequencing from Bacteria)
Sample Type Quantity Quality Shipping Temperature
Bacteria Colonies on agar Circle and number colonies or leave blank for random pick. Individual colonies should be widely spaced on agar plate Ambient or blue ice
Liquid Culture 100ul of overnight-grown liquid culture (8 to 16 hours) Samples should be visibly cloudy Ambient or blue ice
Glycerol Stock 100ul of glycerol stock Samples should be visibly cloudy Dry ice

*Colony-EZ Plus is recommended for customers who require deeper coverage in their sequence, particularly for difficult plasmids. Process involves mini prep of samples prior to extraction to assist. Please expect 1 additional business day for results. Additional charges apply.

Oxford Nanopore Amplicon Sequencing

Order Today
  1. GENEWIZ Drop Box or Fridge – Place your samples and a copy of your order form in a GENEWIZ drop box or fridge before the cut-off time. Call 1-877-436-3949 for your nearest GENEWIZ drop-off location.
  2. GENEWIZ Courier Service – Request for courier pickup if available at your location. Please call 1-877-436-3949 to find out if a GENEWIZ courier picks up at your site. For San Diego customers within the pickup zone, specify the pickup location when placing your order. Note that GENEWIZ couriers pick up room temperature samples only.
  3. If a drop box or courier is not available, and for International shipments, ship your samples via overnight delivery service (FedEx if possible) to us at the following location:

    GENEWIZ from Azenta
    115 Corporate Blvd.
    South Plainfield, NJ 07080
    USA
    908-222-0711
PCR-EZ (Long-Read PCR Product Sequencing)
Sample Type Quantity Quality Shipping Temperature
Unpurified and Purified PCR Products
Water, EB, Low TE buffer (0.1mM EDTA) 		Minimum Amount:
Quantity: ≥500 ng
Volume: ≥10 µL
Concentration: 50 ng/µL Normalized 		A260/A280 = 1.8–2.0 Ambient or blue ice
PCR-EZ Max (Full-Length, Long-Read PCR Product Sequencing)
Sample Type Quantity Quality Shipping Temperature
Unpurified and Purified PCR Products
Water, EB, Low TE buffer (0.1mM EDTA) 		Minimum Amount:
≥100 ng in 10 µL (10 ng/µL) per 1 kb amplicon is required (i.e. a 4 kb amplicon requires 400 ng in 10 µL at 40 ng/µL) 		A260/A280 = 1.8–2.0
RNA-free 		Ambient or blue ice

Amplicon-Based Next Generation Sequencing

Request Quote

Direct Shipping (FedEx):

Please use an overnight delivery service such as FedEx for all NGS samples. A printout of the confirmed quote must be included with the material. View our Sample Submission Best Practices Checklist .

All Amplicon-based Next-Gen Sequencing (except for Amplicon-EZ) direct shipments should be sent to:

GENEWIZ from Azenta Life Sciences
Attn: NGS
111 Corporate Boulevard
Suite H Loading Dock
South Plainfield, NJ 07080
USA

All Amplicon-EZ direct shipments should be sent to:

GENEWIZ from Azenta Life Sciences
115 Corporate Boulevard
South Plainfield, NJ 07080
USA

Tubes / Plates

Organize your samples in tubes or plates following the order indicated on the sample submission form.

Tubes: Prepare samples in clearly labeled and well-organized 1.5 mL flip-cap microcentrifuge tubes. Please avoid using Parafilm to seal the tubes.

Plates: For projects with 16 or more samples, prepare samples in clearly labeled, securely sealed 96-well full-skirted PCR v-bottom plates. Arrange the samples vertically by column (i.e. A1, B1, C1, etc.)

Quality control (QC) is conducted using Qubit (not Nanodrop) and TapeStation. QC results will determine the most suitable approach based on sample quantity and quality.

Illumina Amplicon Sequencing
Sample Type Quantity Quality Shipping Temperature
Genomic DNA Double-Stranded*
Water, EB, low TE buffer (0.1mM EDTA) 		Preferred Amount:
Quantity: ≥250 ng
Volume: 25-50 µL
Concentration: 10 – 50 ng/µL

Minimum Amount:
Quantity: 150 ng
Volume: >25 µL
Concentration: ≥6 ng/µL 		A260/A280 = 1.8-2.0
DIN >5.0
RNA-free 		Dry ice

*Purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids accepted.

PacBio Amplicon Sequencing
Sample Type Quantity Quality Shipping Temperature
Amplicon DNA*
Low TE buffer (0.1mM EDTA) 		Preferred Amount:
Quantity: ≥2 µg
Volume: ≥20 µL
Concentration: ≥50 ng/µL

Minimum Amount:
Quantity: ≥1 µg
Volume: ≥20 µL
Concentration: ≥50 ng/µL 		A260/A280 = 1.8-2.2
A260/A230 = ≥2.0
RNA-free 		Dry ice

*Purified and unpurified amplicons, restriction digested material, and plasmids accepted. Additionally, we can prepare an amplicon from a wide range of starting materials, including genomic DNA, cell pellets, and tissue material. Please see the Extraction Requirements section for further details.

Custom TargetGxOne™ Amplicon Sequencing
Sample Type Quantity Quality Shipping Temperature
Genomic DNA Double-stranded
Water, EB, Low TE buffer (0.1mM EDTA) 		Preferred Amount:
Quantity: ≥250 ng
Volume: 25-50 µL
Concentration: ≥10 ng/µL

Minimum Amount:
Quantity: 100 ng
Volume: ≥25 µL
Concentration: ≥4 ng/µL 		A260/A280 = 1.8-2.0
DIN >7.0
RNA-free 		Dry ice
Amplicon-EZ (AMP-EZ)
Sample Type Quantity Quality Shipping Temperature
Purified PCR Products
Water, EB, Low TE buffer (0.1mM EDTA) 		Minimum Amount:
Quantity: ≥500 ng
Volume: ≥25 µL
Concentration: 20 ng/µL Normalized 		A260/A280 = 1.8-2.0 Ambient or blue ice