PCR-EZ Max
Achieve high-confidence sequencing for both clonal and mixed PCR products with PCR‑EZ MAX, our long-read NGS solution. Using Oxford Nanopore Technologies® (ONT) with a native barcoding workflow, PCR-EZ Max provides deeper sequencing coverage to help characterize mixed amplicons, detect low-frequency variants, and generate comprehensive sequence data for PCR products up to 25 kb.
Easily submit samples through our Dropbox network and advance your research with high-quality, long-read sequencing tailored for complex PCR product analysis.
What is PCR-EZ Max Sequencing
PCR-EZ Max sequencing service determines the DNA sequence of PCR-amplified fragments, including samples that may contain mixed populations or non-clonal amplicons. With deeper coverage, researchers can better evaluate sequence diversity, identify mutations or variants, confirm amplification accuracy, and characterize complex PCR products with greater confidence.
How is PCR-EZ Max Sequencing Done?
PCR-EZ Max utilizes a native barcoding workflow followed by Oxford Nanopore Technologies® (ONT) long-read sequencing. Following sequencing, reads are demultiplexed, quality-filtered, and analyzed throughout our bioinformatics pipeline to generate consensus sequences, coverage metrics, and variant information. The deeper sequencing coverage provided by PCR-EZ Max enables accurate characterization of mixed PCR products by detecting low-frequency variants and sequence heterogeneity.
Mixed PCR Product Workflow with PCR-EZ Max
GENEWIZ Sequencing Service Comparison
| GENEWIZ Service | Starting Material | Technology | Primer | Cost | Read Length | Reads / Read Depth | Best For |
|---|---|---|---|---|---|---|---|
PCR-EZ Max |
Purified or unpurified PCR product | Oxford Nanopore® Long-read NGS | None | $$ | <25 kb | Up to <5,000* |
|
PCR-EZ |
Purified or unpurified PCR product | Oxford Nanopore® Long-read NGS | None | $$ | <25 kb | <1,000 |
|
Amplicon-EZ |
Purified PCR product | Illumina® Short-read NGS | None | $$ | 150–500 bp | <50,000 |
|
*Read count depends on amplicon number and plasmid size. Expect up to 5,000 reads for amplicons under 6 kb.
The GENEWIZ Difference
-
Rapid Turnaround Times
Receive your sequencing results by 1-2 business days
-
Easily Submit Samples
At any one of our convenient dropbox locations
-
Interactive Data Report
Provides an easy way to understand sequencing results
-
High-throughput PCR product-to-data solution for rapid and interactive construct validation
-
Low-cost, unbiased coverage for use in selection of complete valid PCR products
-
Flexible sample types accepted – Purified or Unpurified PCR products
-
Convenient online ordering – Place a PCR-EZ order directly through your GENEWIZ account
Technical Resources
-
Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide
This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.
-
Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.
Deliverables
- FASTQ files
- Read-length and quality report
- Consensus plasmid assembly
- Plasmid annotation files
NGS Platforms
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
