PCR-EZ Product Sequencing
PCR-EZ product sequencing is a long-read NGS-based PCR sequencing service that utilizes Oxford Nanopore Technologies® (ONT) to provide rapid sequencing capabilities of purified or unpurified PCR products up to 25 kilobases (kb) in length. Unlike traditional sequencing technologies (such as Sanger sequencing) which are often limited to sequences of only up to 1,000 base pairs, our PCR-EZ service ensures a high-throughput and cost-effective solution to screen your full entire PCR product and obtain comprehensive sequencing data within 1 business day.
Easily submit samples through our Dropbox network and advance your research with high-quality, long-read sequencing tailored to meet your research needs.
What is PCR Product Sequencing
Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).
PCR product sequencing is the process of determining the exact DNA sequence of a fragment amplified through polymerase chain reaction (PCR). The “PCR product” refers to the PCR-amplified DNA segment, which can then be sequenced and analyzed to confirm amplification accuracy, detect mutations, identify genetic variations, or validate cloning and gene-editing experiments. By sequencing PCR fragments, researchers gain detailed insights into specific genes, study genetic changes, and support applications in molecular diagnostics and research.
How is PCR Product Sequencing done?
PCR product sequencing starts with amplification of the target DNA region using PCR. The PCR-amplified fragment is then purified to remove excess primers, nucleotides, and enzymes before sequencing, commonly via Sanger sequencing or Oxford Nanopore Technology (ONT) Sequencing. Sequencing data is analyzed with bioinformatics tools, allowing researchers to read the nucleotide sequence accurately, verify the amplified DNA, and detect any mutations or variations within the PCR fragment. This method ensures reliable confirmation of PCR results and precise characterization of the DNA region of interest.
PCR Product Workflow with PCR-EZ
Features & Benefits
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Rapid Turnaround Times
Receive your sequencing results by 8 AM the next morning*
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Easily Submit Samples
At any one of our convenient dropbox locations
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Interactive Data Report
Provides an easy way to understand sequencing results
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High-throughput PCR product-to-data solution for rapid and interactive construct validation
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Low-cost, unbiased coverage for use in selection of complete valid PCR products
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Flexible sample types accepted – Purified or Unpurified PCR products
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Convenient online ordering – Place a PCR-EZ order directly through your GENEWIZ account
Technology Comparison: Long Ready PCR Sequencing
| GENEWIZ Service | Starting Material | Technology | Read Quality | Read Length | Reads / Read Depth | Automatic Assembly | Best For |
|---|---|---|---|---|---|---|---|
Sanger Sequencing |
Purified or unpurified PCR product | Sanger | Higher Quality | <1000 bp | 1–4 | — |
|
Amplicon-EZ |
Purified PCR product | Illumina® Short-read NGS | Best Quality | 150–500 bp | 50,000 | Included |
|
PCR-EZ |
Purified or unpurified PCR product | Oxford Nanopore® Long-read NGS | Good Quality | <25 kb | 1,000 | Included |
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Long-Read Amplicon Sequencing |
Purified PCR product | PacBio® Long-read NGS | Best Quality | <25 kb | 100,000 | Included |
|
Technical Resources
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Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide
This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.
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Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.
Deliverables
- FASTQ files
- Read-length and quality report
- Consensus plasmid assembly
- Plasmid annotation files
NGS Platforms
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
