AAV Gene Therapy Sequencing
Adeno-associated virus (AAV) gene therapy sequencing enables you to verify the quality and integrity of packaged AAV vectors, including inverted terminal repeat (ITR) regions, to assess purity, clonality and fidelity of packaged products.
Our solutions combine next generation sequencing (NGS) using the latest technologies, including Illumina® short-read, PacBio® long-read, and Oxford Nanopore Technologies® long-read sequencing, with our proprietary QC and library preparation processes to provide you with reliable, accurate results and streamline your AAV gene therapy research.
Why is AAV Sequencing Important?
Recombinant adeno-associated viruses (rAAV) are preferred in gene therapy due to their non-replicating nature, high titers, low immunogenicity, and low genotoxicity. Comprehensive quality control (QC) is crucial to maintain virus integrity and purity.
Next generation sequencing, combined with our proprietary QC process, helps researchers ensure the integrity of packaged AAV vectors.
AAV-ANCED STRATEGIES
For Adeno-Associated Virus in Gene Therapy
✓ Reliable quality control and safety testing for AAV development
✓ Strategies to read through inverted terminal repeats in AAV
✓ High-fidelity methods for AAV plasmid prep and packaging
✓ Advanced sequencing for AAV quality and safety monitoring
AAV Sequencing Applications
Illumina® short-read, PacBio® long-read and Oxford Nanopore Technologies® long-read sequencing can be used to determine:
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FIDELITY
Exact full-length product of particle population -
PURITY
Detection of contaminating products -
CLONALITY
Count values for number of sequences in sample
AAV GENOME SEQUENCING WORKFLOW
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1. Sample Preparation
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2. Library Preparation
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3. Sequencing
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4. Bioinformatics Analysis
The GENEWIZ Difference
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Superior Data Quality
Exceeding manufacturer’s benchmarks -
Real-Time Project Updates
Through our online system -
Ph.D.-Level Support
At every step
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Trusted partner supporting over 150 top global pharmaceutical and biotech customers in their cell and gene therapy research
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Optimized, automated, and scalable workflows with stringent quality control producing high-quality, consistent results
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RUO and CLIA-grade, high-capacity and high-throughput sequencing for projects of virtually any size and sample type
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Customized and extensive bioinformatics solutions with interactive, hands-on analysis
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eBook | A Guide to AAV in Gene Therapy
AAV vectors play a critical role in gene therapy, offering an ideal approach for targeted gene delivery. However, understanding the complexities of AAV packaging, optimization, and quality control testing can be challenging. In this eBook, learn step-by-step strategies for mastering AAV vector development and enhancing your gene therapy applications.
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Webinar | Accelerating AAV Gene Therapy Development
In this webinar, co-hosted by GENEWIZ and Form Bio, learn how to accelerate your AAV gene therapy development with an end-to-end solution that combines long-read sequencing with genome integrity analysis. Discover how this integrated workflow delivers actionable insights for your research, enabling reduced complexity and accelerated timelines from vector design to lead candidate selection.
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Article | The Long and Short of AAV Sequencing: Enhancing Gene Therapy Pipelines with Orthogonal Analysis
Each sequencing platform offers unique strengths that address specific challenges in the gene therapy workflow. This article explores how long- and short-read technologies can complement each other and their critical role in the gene therapy landscape. By understanding their unique strengths and economic trade-offs, you’ll discover how to strategically deploy these tools to achieve robust results.
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Webinar | A Comprehensive Guide to Using AAV Vectors in Gene Therapy
Working with AAV vectors can be challenging. In this on-demand workshop & roundtable discussion, you’ll gain a better understanding of the AAV development pipeline for gene therapy research and learn how to optimize upstream and downstream processes including AAV synthesis, sequencing, bioinformatics, and storage.
NGS PLATFORMS
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ from Azenta does not guarantee data output or quality for sequencing-only projects.
