Oligos Retained for Sanger
GENEWIZ Oligos Retained for Sanger service eliminates the need to coordinate between vendors before sending your samples for Sanger sequencing. We synthesize, dilute, organize, and store your primers for up to a year for use with future Sanger sequencing orders. This service is designed for the bench scientist with simplicity and convenience in mind.
How are oligos used in Sanger sequencing?
Oligos are used in Sanger sequencing as primers to initiate DNA synthesis and as terminal nucleotides to terminate further DNA synthesis.
Features & Benefits
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Save time on ordering and aliquoting primers
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Standard, desalted oligos up to 60 nt at 25 or 50 nmol scale
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Includes storage at GENEWIZ for up to one year
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Expedited turnaround available for Boston-area researchers
Turnaround Time
| Order Placed | US (Non-Boston Area) | |
|---|---|---|
| Before 3 PM ET | Ready for sequencing the next business day* | |
| Order Placed | Greater Boston Area | |
| Before Noon | Ready same-day for overnight sequencing* | |
| Noon – 8 PM | Ready for sequencing the next business day* | |
*Larger orders or sequencing oligos longer than 30 bases may require additional turnaround time. Oligos Retained for Sanger will be transferred internally for GENEWIZ sequencing projects. Please contact dnaseq@azenta.com for more information.
Technical Resources
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Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.