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Targeted PCR Sequencing

 

GENEWIZ Targeted PCR Sequencing customizes your Sanger sequencing experience with PCR + Sanger solutions from the leader in DNA sequencing. This fully customizable service allows you to streamline your workflow and extend your bench with your choice of PCR plus Sanger services with data delivered in as few as 3 days with our pre-developed assays. These services support a wide-range of applications including targeted microbial/fungal sequencing, NGS data confirmation, antibody heavy/light chain sequencing, mutation detection analysis, cell bank characterization, SNP genotyping and more. Explore the range of services we offer below. In our ordering system, you can find these services under Molecular Genetics (PCR + Sanger).

Contact GENEWIZ Customer Support to discuss your project and to learn more about our many offerings.



Molecular Genetics (PCR + Sanger) Services



Targeted PCR Sequencing Services

▼Mutation Analysis Service

Characterizing mutations is an important technique in the molecular biologist’s toolbox. GENEWIZ Mutation Analysis service helps scientists ramp up mutation detection in coding exons, enabling scientists to quickly analyze and identify mutations that may affect the function of their gene of interest.

Our Mutation Analysis service includes PCR optimization, amplicon production and purification, and Sanger sequencing for your CRISPR regions of interest. Whether you are confirming that a mutation has occurred or precisely determining the genotype and specific allelic event, leveraging our expertise ensures that your genome editing functions efficiently.

Starting Material Assay Coverage Confirm/Identify Deliverables/Reports
gDNA (extractions available) Full Coding Exons, Coding Exon of Interest, Introns SNPs
Mutations
Indels		 Raw data files: .ab1, .seq
Nucleotide level mutations
Amino acid changes*

*Available upon request

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▼SNP Genotyping Service

Genotyping is a method used to determine the genetic makeup of an organism. This method identifies genetic variations by comparing an individual’s sequence against a wild-type reference sequence to analyze single nucleotide polymorphisms (SNPs). SNPs are present in both the coding and non-coding regions of a gene and are one of the most common types of variants; they are represented as a single nucleotide mutation within a DNA sequence.

GENEWIZ’s SNP Genotyping service is a PCR and Sanger sequencing-based solution that is used to ramp up SNP screening assays and validate SNPs of interest with speed and accuracy.

Starting Material Assay Coverage Confirm/Identify Deliverables/Reports
gDNA (extractions available) Annotated SNPs of interest SNPs Raw data files: .ab1, .seq
Nucleotide level mutations

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▼Yeast Colony Sequencing

GENEWIZ Yeast Colony Sequencing can quickly sequence DNA from yeast colonies supplied on an agar plate. Simply send us your yeast colonies and your sequencing data will be delivered to your online account. No DNA extraction or PCR is required on your part.

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Additional PCR Sequencing Services

▼Confirmatory Sequencing & Cell Bank Characterization

GENEWIZ Sanger-based confirmatory sequencing services can be customized to detect your endogenous or recombinant transcripts/genes, support cell line development, monoclonal antibody heavy chain/light chain sequence confirmation, and transfection verification.

Our experienced scientists will design, develop, and optimize your assays to verify the identity of your sequences and provide you with the final data. We can even utilize samples submitted from existing GENEWIZ Next Generation Sequencing projects.

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▼Fragment Analysis Service

GENEWIZ provides fast and reliable fragment analysis services to researchers across all molecular disciplines. Capillary-based fragment analysis allows for accurate, high-throughput analysis of a population of DNA fragments. This service is performed on ABI3730xl’s for accurate size calling, consistent band intensities, and low run-to-run migration variations. Eliminate the pain of polyacrylamide gels and let GENEWIZ provide fast, reliable, and accurate results for your FA projects.

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▼Nucleic Acid Extraction

GENEWIZ provides nucleic acid extraction as part of complete projects and as a standalone service. Simply provide GENEWIZ with your sample and we do the rest— delivering you a high-quality extracted product or characterization of the extracted material in a downstream application on-site. Regulatory grade extraction services are also available – please email for additional information.

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Featured Predeveloped Assays

Elevate your study of intricate biological pathways with pre-developed assays designed to help you advance your research. With a range of pre-developed assays targeting key genes such as BRCA1/2, TP53, HTT, and FOXP2, you can confidently focus on accelerating your breakthroughs across applications including oncology, neurology, and autoimmune disorders.

▼Predeveloped Assays List

Gene Details
BRCA2 Hereditary Breast Cancer, solid tumors, oncology
BRAF MAPK signaling, oncology
HRAS HRAS signaling, MAPK signaling, oncology
IDH1 Cellular metabolism, oncology
IDH2 Cellular metabolism, oncology
KRAS RAS signaling, MAPK signaling, oncology
MAP2K1 RAS signaling, MAPK signaling, oncology
MAP2K2 RAS signaling, MAPK signaling, oncology
MAPK1 RAS signaling, MAPK signaling, oncology
NRAS RAS signaling, MAPK signaling, oncology
CFTR Hereditary, Respiratory or pulmonary disorder
PIK3CA PI3K signaling, mTor signaling, solid tumors, oncology
PTEN Tumor suppressor, solid tumors, oncology
TP53 Oncology
APOE Alzheimer’s disease, dementia, neuropathology
EGFR Oncology, lung cancers, solid tumors



Features & Benefits

Reliable Assay Development: Our highly-trained scientists develop PCR assays targeted to your region of interest.
Flexible Starting Materials: GENEWIZ can start with purified genomic DNA templates or extract it from supplied sources.
High-Throughput Processing: Target regions are amplified using optimized assays; amplicons are purified and sequenced by capillary electrophoresis in our best-in-class facility.
Fast Turnaround: Data delivery starting at 3 business days after assay development.
Accurate Data Analysis: Mutations are identified with both automated and manual analysis to ensure accuracy.
Superior Support: Dedicated GENEWIZ scientist, expert technical support, and consistent, transparent communication with weekly project updates


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