GENEWIZ provides reliable, award-winning Sanger DNA Sequencing services to researchers at academic, pharmaceutical, GLP, biotechnology, and government institutions. Sanger sequencing from purified templates is our most popular template type. Service levels available for purified templates include Pre-Mixed, Pre-Defined, and Custom sequencing.
GENEWIZ offers three service levels for Sanger sequencing of purified plasmid and PCR templates: Pre-mixed, Pre-defined, and Custom.
To determine the service that is best for your samples, please see the descriptions for each service below.
If you need help choosing your optimal Sanger service level, please contact us:
By choosing our Pre-Defined Service, you submit your template and primer in separate tubes, and a GENEWIZ scientist will mix them when your sample is received. An additional charge applies in most cases.
For sequencing plasmids or purified PCR products, submit your template and primer in separate tubes according to our Sample Submission Guidelines.
This is a full-service option for sequencing of purified plasmids and purified PCR product samples with unknown concentration. For purified plasmids column-based PCR purifications, a GENEWIZ scientist will measure and adjust the concentration of your template to optimize the sequencing reaction.
Note that if you have enzymatically-purified PCR products, we recommend that you submit these samples under the Pre-Mixed or Pre-Defined service levels. An accurate OD cannot be obtained from an enzymatically-purified template.