PURIFIED TEMPLATES

GENEWIZ provides reliable, award-winning Sanger DNA Sequencing services to researchers at academic, pharmaceutical, GLP, biotechnology, and government institutions. Sanger sequencing from purified templates is our most popular template type. Service levels available for purified templates include Pre-Mixed, Pre-Defined, and Custom sequencing.


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SANGER SEQUENCING OF PURIFIED TEMPLATES

GENEWIZ offers three service levels for Sanger sequencing of purified plasmid and PCR templates: Pre-mixed, Pre-defined, and Custom.
To determine the service that is best for your samples, please see the descriptions for each service below.

If you need help choosing your optimal Sanger service level, please contact us:

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 2) | Live Chat.


Pre-Mixed

For sequencing plasmids or purified PCR products, this service is the most economical choice.

You provide your template and primer mixed in the same tube according to our Sample Submission Guidelines.

Note: A GENEWIZ Universal Primer can be used in this service at no charge.



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Pre-Defined

By choosing our Pre-Defined Service, you submit your template and primer in separate tubes, and a GENEWIZ scientist will mix them when your sample is received. An additional charge applies in most cases.

For sequencing plasmids or purified PCR products, submit your template and primer in separate tubes according to our Sample Submission Guidelines.

Note: A GENEWIZ Universal Primer can be used in this service at no charge.



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Custom

This is a full-service option for sequencing of purified plasmids and purified PCR product samples with unknown concentration. For purified plasmids column-based PCR purifications, a GENEWIZ scientist will measure and adjust the concentration of your template to optimize the sequencing reaction.

Note that if you have enzymatically-purified PCR products, we recommend that you submit these samples under the Pre-Mixed or Pre-Defined service levels. An accurate OD cannot be obtained from an enzymatically-purified template.

Submit template and primer separately according to our Sample Submission Guidelines.

Note: A GENEWIZ Universal Primer can be used in this service at no charge.



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Features & BENEFITS

High Quality

DNA sequencing read lengths ~1000 bases and at least 500 bases Phred20. A typical read will provide 800 bases Phred 20.  Quality Score and Contiguous Read Length (CRL) provided in each read

Convenient

Contact us to locate a drop box or pick-up service near you for free shipping

Best-in-Class Difficult Template Protocols

For hairpins and GC-rich DNA

Free Vector Primers

Wide selection of GENEWIZ Universal Primers are available for most standard vectors

Technical Support

Available at no charge to discuss your project, troubleshoot, and answer questions 8 AM – 8 PM Eastern Time, Monday – Friday; results are posted on Saturdays for weekend workers

Online System

24/7 web-based online ordering and data retrieval, with bar-coded order tracking

Technology

Analysis using ABI 3730xl DNA Analyzers for capillary electrophoresis and fluorescent dye terminator detection

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.



Email | Phone (1-877-GENEWIZ (436-3949), Ext. 2) | Live Chat