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Primer Walking Sample Submission Guidelines


For the best results, GENEWIZ strongly recommends you follow our Sample Submission Guidelines as closely as possible. If you have any questions, our Primer walking team is here to help!

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Primer Walking Sample Submission Guidelines

Plasmid - Submit 1 µg per kb of target sequence for single stranded coverage and 2 µg per kb sequence for double stranded coverage. Submit at least 10 µg plasmid if region of interest is of unknown length.

Primers - GENEWIZ can synthesize the initial primer for you if the primer sequence is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.

Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and label them with sample or primer name and concentration.

Shipping (US) - Send samples to GENEWIZ via Standard Overnight service or contact us at primerwalking@genewiz.com to find out if there is a GENEWIZ Drop Box or courier pickup service available for your location.

Shipping (EU) - Contact customer service on DNASeqUK@genewiz.com +44 (0) 1279 873 852 for more information

 

Sequence Confirmation Sample Submission Guidelines

Plasmid - Submit 1 µg per kb of target sequence for single stranded coverage and 2 µg per kb sequence for double stranded coverage.

Primers - GENEWIZ can synthesize the initial primer for you if the primer sequence is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.

Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and label them with sample or primer name and concentration.

Shipping (US) - Send samples to GENEWIZ via Standard Overnight service or contact us at primerwalking@genewiz.com to find out if there is a GENEWIZ Drop Box or courier pickup service available for your location.

Shipping (EU) - Contact customer service on DNASeqUK@genewiz.com +44 (0) 1279 873 852 for more information

 

Ship to:

Attn: Molecular Genetics - PW
115 Corporate Blvd
South Plainfield, NJ 07080