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Streamlined Colony Sequencing + Plasmid DNA Prep

Optimize your research and budget with our workflow starting directly from your bacterial clones!

US Premium Services Sample Submission Guidelines


Premium Services


For the best results, GENEWIZ strongly recommends you follow our Sample Submission Guidelines as closely as possible. If you have any questions, our Primer walking team is here to help!

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)


Primer walking: service options

Primer Walking: Sequence Discovery

• Plasmid - Submit 1 µg per kb of target sequence for single-stranded coverage, 2 µg per kb sequence for double-stranded coverage, and 4 µg per kb for 4x coverage. Submit at least 10 µg plasmid if region of interest is of unknown length. 


• PCR Products 
- Submit 10-40 ng per kb of amplicon.

• ITRs - For ITR containing plasmids and PCR products, please submit an additional 1 µg of material.

• Primers - GENEWIZ can synthesize the initial primer if the sequence for a known region (e.g. antibiotic resistance) is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.

• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and label them with sample or primer name and concentration.

 

Primer Walking: Sequence Confirmation

• Plasmid - Submit 1 µg per kb of target sequence for single-stranded coverage, 2 µg per kb sequence for double-stranded coverage, and 4 µg per kb for 4x coverage.

• PCR Products - Submit 10-40 ng per kb of amplicon.

• ITRs - For ITR containing plasmids and PCR products, please submit an additional 1 µg of material.

• Primers - GENEWIZ can synthesize all of the primers if the known reference sequence is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.

• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and label them with sample or primer name and concentration.

 

Shipping

US - Send samples to GENEWIZ NJ via standard overnight service

Ship to:
Attn: Molecular Genetics - PW
115 Corporate Blvd
South Plainfield, NJ 07080
USA

 

OR contact us at primerwalking@genewiz.com to find out if there is a GENEWIZ Drop Box or courier pickup service available for your location.