Immune Profiling for Immunogenomics
Immunogenomics is the study of gene regulatory mechanisms and gene mutations involved in disease-associated immune responses. Immune profiling using next generation sequencing (NGS) allows for ultra-deep immune repertoire analysis. This approach uncovers the composition of B-cell receptors (BCRs) and T-cell receptors (TCRs) generated through the recombination of the V(D)J loci, which is crucial to the adaptive immune system.
For in vivo natural antibody populations, we offer single-cell sequencing for physical pairing of the VH/VL or α/β chains of each complex or bulk sequencing for non-physical pairing inferred through frequency analysis. For in vitro recombinant antibody populations, short- and long-read options are available for unpaired and paired analyses. Add custom bioinformatics to any configuration to comprehensively characterize the entire BCR or TCR repertoire.
Immunosequencing Options
Our suite of immunogenomics services provide next generation sequencing solutions for natural and recombinant antibody populations to achieve high-throughput analysis of diverse populations. Options include physical pairing of the VH/VL or α/β chains of each complex and non-physical pairing inferred through frequency analysis. Find the optimal solution for your project below.
| Antibody Discovery Platform | Frequency Analysis | Physical Pairing | Sequence Target | Applications |
|---|---|---|---|---|
In vivo |
Immuno-Profiling | Single-Cell Immuno-Profiling | B-cell receptors (BCR) T-cell receptors (TCR) |
BCR/TCR repertoire profiling In vivo antibody discovery Hybridoma screening |
In vitro |
Immuno-Profiling | Long-Read Amplicon | Antigen-binding fragments (Fab) Single-chain variable fragments (scFv) |
In vitro antibody discovery via surface display |
Immunosequencing Workflows
Immuno-Profiling and short-read workflows
Immuno-Profiling
Perform bulk analysis of V(D)J sequences at the RNA level.
Short-Read Amplicon Sequencing
Assay the diversity of clones after bio-panning in surface display experiments. Heavy and light chains, amplified separately, are fully sequenced on the Illumina MiSeq platform. We offer the following NGS services for this approach:
Single-cell and long-read workflows
Single-Cell Immuno-Profiling
Pair BCR and TCR chains at single-cell resolution using 10x Genomics™ technology.
Long-Read Amplicon Sequencing
Analyze clonal diversity after bio-panning via long-read sequencing technology. Heavy and light chains amplified together in a single PCR product are contiguously sequenced using the PacBio Sequel® platform. This approach physically pairs the sequences of the two chains.
NGS Platforms
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
