Immunogenomics

The adaptive immune system employs an immensely diverse repertoire of lymphocyte receptors to recognize and neutralize foreign agents. Analyzing the composition of B- and T-cell receptors in vivo can provide insight into diseases and aid the development of biologics and vaccines. Surface display technology can also be used as a complementary or alternative approach to discover antibodies, including both antigen-binding (Fab) and single-chain variable fragments (scFv), targeted against an antigen.

Why GENEWIZ   
Building

Service Options

Our suite of immunogenomics services provide next generation sequencing solutions for natural and recombinant antibody populations to achieve high-throughput analysis of diverse populations. Options include physical pairing of the VH/VL or α/β chains of each complex and non-physical pairing inferred through frequency analysis. Find the optimal solution for your project below.

Antibody Discovery
Platform 
Method of Pairing Chain Sequences Sequence Target Applications
Frequency
Analysis
Physical
Pairing
In vivo Immuno-Profiling Single-Cell
Immuno-Profiling

B-cell receptors (BCR)

T-cell receptors (TCR)

BCR/TCR repertoire
profiling

In vivo antibody
discovery

In vitro Short-Read Amplicon
Sequencing
Ultra-Long Amplicon
Sequencing

Antigen-binding
fragments (Fab)

Single-chain variable
fragments (scFv)

In vitro antibody
discovery via surface
display

Service Details and Workflows

Immuno-Profiling

Perform bulk analysis of V(D)J sequences at the RNA level.



Short-Read Amplicon Sequencing

Assay the diversity of clones after bio-panning in surface display experiments. Heavy and light chains, amplified separately, are fully sequenced on the Illumina MiSeq platform. We offer the following NGS services for this approach:

Single-Cell Immuno-Profiling

Pair BCR and TCR chains at single-cell resolution using 10x Genomics™ technology.


Ultra-Long Amplicon Sequencing

Analyze clonal diversity after bio-panning via long-read sequencing technology. Heavy and light chains amplified together in a single PCR product are contiguously sequenced using the PacBio Sequel® platform. This approach physically pairs the sequences of the two chains.