The adaptive immune system employs an immensely diverse repertoire of lymphocyte receptors to recognize and neutralize foreign agents. Analyzing the composition of B- and T-cell receptors in vivo can provide insight into diseases and aid the development of biologics and vaccines. Surface display technology can also be used as a complementary or alternative approach to discover antibodies, including both antigen-binding (Fab) and single-chain variable fragments (scFv), targeted against an antigen.



Immunosequencing Options

Our suite of immunogenomics services provide next generation sequencing solutions for natural and recombinant antibody populations to achieve high-throughput analysis of diverse populations. Options include physical pairing of the VH/VL or α/β chains of each complex and non-physical pairing inferred through frequency analysis. Find the optimal solution for your project below.

Antibody Discovery
Method of Pairing Chain Sequences Sequence Target Applications
In vivo Immuno-Profiling Single-Cell

B-cell receptors (BCR)

T-cell receptors (TCR)

BCR/TCR repertoire

In vivo antibody

Hybridoma screening

In vitro Short-Read Amplicon
Long-Read Amplicon

fragments (Fab)

Single-chain variable
fragments (scFv)

In vitro antibody
discovery via surface

Immunosequencing Workflows


Perform bulk analysis of V(D)J sequences at the RNA level.

Short-Read Amplicon Sequencing

Assay the diversity of clones after bio-panning in surface display experiments. Heavy and light chains, amplified separately, are fully sequenced on the Illumina MiSeq platform. We offer the following NGS services for this approach:

Single-Cell Immuno-Profiling

Pair BCR and TCR chains at single-cell resolution using 10x Genomics™ technology.

Long-Read Amplicon Sequencing

Analyze clonal diversity after bio-panning via long-read sequencing technology. Heavy and light chains amplified together in a single PCR product are contiguously sequenced using the PacBio Sequel® platform. This approach physically pairs the sequences of the two chains.