Yeast Colony Sequencing

GENEWIZ can quickly sequence DNA from yeast colonies supplied on an agar plate.  Simply send us your yeast colonies and your sequencing data will be delivered to your online account.  No DNA extraction or PCR is required on your part.


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GENEWIZ Yeast Colony Sequencing Services

GENEWIZ can help streamline your yeast genetics sequencing projects. Avoid time-consuming DNA preps from yeast by utilizing GENEWIZ yeast colony sequencing services. Let GENEWIZ expedite your yeast colony screening projects or fast-track your yeast genetic modification experiments.


Features and Benefits

Target transformed plasmids or integrated regions in the yeast genome
PCR amplification directly from yeast colonies
Forward and reverse sequencing for your gene or region of interest. You supply your own primers and PCR conditions, or let GENEWIZ custom design primers to your target
Fast & reliable turnaround times
Robust PCR protocols and high quality sequencing data performed at our award-winning facility
Experienced Ph.D. scientists are available to answer questions and discuss your project in detail

Yeast Colony Sequencing Process


  • Starting Material: Yeast Colony


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  • PCR



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  • Sanger
    Sequencing



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Colony is picked from plate for PCR amplification.
Quality Control: PCR product is observed by agarose gel electrophoresis.
Gene of interest is amplified by PCR using custom designed or supplied primers.
Resuling PCR product is sequenced in both directions,
Deliverables: Raw data in ab1 and seq files.

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)