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Azenta Life Sciences, formerly GENEWIZ can quickly sequence DNA from yeast colonies supplied on an agar plate. Simply send us your yeast colonies and your sequencing data will be delivered to your online account. No DNA extraction or PCR is required on your part.
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Azenta Yeast Colony Sequencing Services
Azenta can help streamline your yeast genetics sequencing projects. Avoid time-consuming DNA preps from yeast by utilizing Azenta yeast colony sequencing services. Let Azenta expedite your yeast colony screening projects or fast-track your yeast genetic modification experiments.
Features and Benefits
Target transformed plasmids or integrated regions in the yeast genome
PCR amplification directly from yeast colonies
Forward and reverse sequencing for your gene or region of interest. You supply your own primers and PCR conditions, or let Azenta custom design primers to your target
Fast & reliable turnaround times
Robust PCR protocols and high quality sequencing data performed at our award-winning facility
Experienced Ph.D. scientists are available to answer questions and discuss your project in detail
Yeast Colony Sequencing Process
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Starting Material: Yeast Colony
1 -
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PCR
2 -
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Sanger
Sequencing
3
Colony is picked from plate for PCR amplification.
Quality Control: PCR product is observed by agarose gel electrophoresis.
Gene of interest is amplified by PCR using custom designed or supplied primers.
Gene of interest is amplified by PCR using custom designed or supplied primers.
Resuling PCR product is sequenced in both directions,
Deliverables: Raw data in ab1 and seq files.
Deliverables: Raw data in ab1 and seq files.
HOW TO ORDER
*Samples must arrive at the Azenta New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.
