AAV-ITR SANGER SEQUENCING

 

GENEWIZ’s new proprietary Sanger sequencing method sequences through difficult inverted terminal repeat (ITR) regions of adeno-associated virus (AAV), to expedite screening and validation of leads for your cell and gene therapy research. 

This newly-developed protocol prevents abrupt reduction in the sequencing signal at the start of the ITR hairpin and reads through the full length of the ITR region. AAV-ITR sequencing can be combined with our Primer Walking service to sequence whole AAV vectors.

 

 

 

GENEWIZ AAV-ITR Sequencing Provides a Breakthrough Solution

Adeno-associated virus (AAV) is one of the most actively investigated gene therapy vehicles. GENEWIZ’s robust AAV-ITR Sequencing Protocol sequences through its difficult inverted terminal repeat (ITR) regions to better support your viral vector testing and drug discovery pipeline.


Standard Protocol

GENEWIZ AAV-ITR Protocol


AAV-ITR Sequencing – GENEWIZ Proprietary Protocol

GENEWIZ AAV-ITR Protocol

FEATURES & BENEFITS

Qualitative assessment of the integrity of wild-type and partially-truncated ITR regions in AAV plasmids 
Ph.D.-level customer support throughout the project
Turnaround time starting at just 5 business days
Increased read lengths and improved data quality for early detection of point mutations
Advanced network of dropboxes and couriers for convenient sample submission
Online ordering system allows real-time order management
AAV ITR Sequencing Case Study

Tech Note: Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-associated Virus (AAV)

 

GENEWIZ has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids.

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.



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