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High-Quality Plasmids to Meet Tight Deadlines



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  Sample Submission Guidelines
  

PLASMID DNA PREPARATION

Speed, quality, and flexibility of your Plasmid DNA Preparation service are critical factors for any plasmid purification procedure. At GENEWIZ, delivering high quality DNA from a variety of starting materials, including custom strains, is our priority. Our Plasmid DNA Preparation teams provide high quality plasmid with reliable, fast turnaround to accommodate tight deadlines.  Service is offered per-run, from mini- to giga-scale, covering the microgram to milligram range. We also provide custom large-scale Plasmid DNA Preparation to a specific yield, for the milligram to gram range.
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Plasmid DNA Prep - From mini to giga-scale, we're here to help

Get up to 25% off your first order

Choice of Starting Material

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DNA

Transformation provided as a service*

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Glycerol Stock

Provide 200 μL frozen stock

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Bacterial Colony

Please provide fresh colonies (<3 days old)

*For DNA samples, GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.

SERVICE OPTIONS



Sequence confirmation of your sample and/or prep.

By request DNA sample storage for convenient re-ordering.


Isolation by sample streaking available.


Aliquotting and custom tube handling available.


DNA suspension in a selection of buffers available.


Endotoxin-free, for the most demanding applications.


DNA normalization available.


Modified culture conditions are possible for low-copy or toxic constructs.


Good Laboratory Practice (GLP) Service available. For drugs advancing to clinical trials - click here for more information.

ANALYSIS OPTIONS

Restriction digest available
Agarose gel analysis available
Multiple levels of sequence confirmation available
Sterility testing available

FULL SPECTRUM OF DNA PREPARATION SERVICES OFFERED

Prep Scale Typical Yield for High Copy Vectors*
Mini (tube)
7-10 μg
Mini (96 well)
5-10 μg
Midi 70-100 μg
Maxi 350-500 μg
Mega 1.5-2.5 mg
Giga 7-10 mg
> Giga > 10 mg

* Actual yield will vary with the nature of the plasmid, bacterial hosts, and other factors.
* Above yield available for U.S. orders only.

Turnaround time for Plasmid DNA Preparation is 2-3 business days when starting from bacterial colonies or glycerol stocks. Turnaround time for Plasmid DNA Preparation is 3-4 days when starting from plasmid DNA. Additional time may be required for service and analysis options, large orders, and shipping.




HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that
direct-sequencing templates are not available for our Same Day service.


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3) | Live Chat