Gene Fragments are double-stranded, linear DNA fragments of 100-3,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that Azenta Life Sciences, formerly GENEWIZ has developed for standard gene synthesis products. We provide Gene Fragments in a lyophilized form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.


How are gene fragments made?

Construction begins with the base-by-base synthesis of oligonucleotides (oligos), followed by assembly into double-stranded DNA (dsDNA) fragments. These custom DNA fragments can be used directly, cloned into vectors, or assembled into larger constructs to serve a variety of research uses.

Gene Fragment Synthesis Features and Benefits

Cloning accuracy: More than 85% of the recombinant colonies from cloning Gene Fragments will contain your desired insert

Easy gene assembly: Easily assemble multiple gene fragments to create a larger construct for downstream applications

Fast delivery time: Fast turnaround time starting in just a few days to meet your research deadlines

Affordable: Very cost-effective to meet your strict budget constraints

Quality control: Stringent quality control process to ensure high sequence fidelity

Ease of use: Sanger sequencing from Azenta Life Sciences, with an extensive worldwide network of dropboxes, makes identifying the correct clones faster and more convenient

Gene Fragment Turnaround Time

Sequence Length* Quantity Completion Time** Predicted Cloning Accuracy
100-500 bp 500 ng 2-4 business days >85% 
501-750 bp 3-5 business days
751-1,000 bp 1,000 ng
1,001-1,500 bp 4-6 business days
1,501-2,250 bp 6-8 business days
2,251-3,000 bp


*Only sequences with overall GC content between 20% and 80% qualify for Gene Fragments service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time.

Gene Block Quality Control

  • Size verification by gel electrophoresis
  • Sequence confirmation via Sanger sequencing

Gene Fragment Synthesis Applications

  • Assemble multiple gene fragments to make full-length gene constructs for protein expression and purification
  • gRNA expression cassettes for CRISPR/Cas9-based gene editing
  • Donor constructs for gene editing experiments
  • Template for in vitro transcription


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Gene Fragment Assembly Deliverables

Standard deliverables include 500-1,000 ng of lyophilized double-stranded DNA, depending on sequence length.

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*Samples must arrive at the Azenta New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.

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