Gene Fragments

 

Gene Fragments are double-stranded, linear DNA fragments of 100-3,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that GENEWIZ from Azenta has developed for standard gene synthesis products. We provide Gene Fragments in a dried form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.

More variants. Faster iteration. Rapid engineering.​


How are gene fragments made?

Construction begins with the base-by-base synthesis of oligonucleotides (oligos), followed by assembly into double-stranded DNA (dsDNA) fragments. These custom DNA fragments can be used directly, cloned into vectors, or assembled into larger constructs to serve a variety of research uses.


Gene Fragment Synthesis Features and Benefits

Cloning accuracy: More than 85% of the recombinant colonies from cloning Gene Fragments will contain your desired insert

Easy gene assembly: Easily assemble multiple gene fragments to create a larger construct for downstream applications

Industry-leading turnaround time: GENEWIZ Fast Fragments ship to your lab as fast as 1 business day​

Affordable: Very cost-effective to meet your strict budget constraints

Quality control: Stringent quality control process to ensure high sequence fidelity

Ease of use: GENEWIZ Sanger sequencing, with an extensive worldwide network of dropboxes, makes identifying the correct clones faster and more convenient

Gene Fragment Services

Expedited Gene Fragments (Fast Fragments)​​

Ships in 1 business day

Standard Gene Fragments (FragmentGENE)​

Ships in 2-3 business days

Built for speed, scale, and scientific rigor

GENEWIZ Double-stranded DNA Fragments (100–3,000 bp) are delivered dried and clone-ready. Choose Fast Fragments (ships in as little as 1 business day) or our standard FragmentGENE service. Synthesis and QC are 100% US-based.

Typical workflow:

  1. Design your fragment(s) or variant set
  2. Select speed — Fast (1-BD) or Standard, choose delivery format
  3. Resuspend & assemble — move directly to cloning, screening, or edits

Gene Fragment Turnaround Time

▼Fast Fragments

Sequence Length* Quantity Completion Time** Predicted Cloning Accuracy
100-250 bp ≤ 47 samples 1-2 business days >85%
251-500 bp ≤ 47 samples 2-3 business days >85%
100-250 bp ≥ 48 samples 1-2 business days >85%
251-500 bp ≥ 48 samples 2-3 business days >85%

*Only sequences with overall GC content between 20% and 80% qualify for Gene Fragments service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time.
**Orders typically require an additional 2-3 BD shipping from our production facilities.


▼FragmentGENE

Sequence Length* Quantity Completion Time** Predicted Cloning Accuracy
100-500 bp 500 ng 2-4 business days >85%
501-750 bp 500 ng 3-5 business days >85%
751-1,000 bp 1,000 ng 3-5 business days >85%
1,001-1,500 bp 1,000 ng 4-6 business days >85%
1,501-2,250 bp 1,000 ng 6-8 business days >85%
2,251-3,000 bp 1,000 ng 6-8 business days >85%

*Only sequences with overall GC content between 20% and 80% qualify for Gene Fragments service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time.
**Orders typically require an additional 2-3 BD shipping from our production facilities.


Gene Block Quality Control

  • Size verification by gel electrophoresis
  • Sequence confirmation via Sanger sequencing

Gene Fragment Synthesis Applications

  • Assemble multiple gene fragments to make full-length gene constructs for protein expression and purification
  • gRNA expression cassettes for CRISPR/Cas9-based gene editing
  • Donor constructs for gene editing experiments
  • Template for in vitro transcription
  • Antibody discovery at scale: CDR mutagenesis, affinity maturation, humanization panels
  • High-throughput screening: barcoded fragments, reporter cassettes, assay controls
  • Protein engineering: linkers/tags/fusions, alanine/tiling scans, domain swaps
  • Alanine/tiling mutagenesis to map function and liability​
  • CDR shuffling & combinatorial libraries for scFv/Fab/IgG​
  • Display library inserts: yeast, phage, mammalian
  • Reporter & MPRA payloads for promoter/UTR tuning​
  • Barcoded controls & spike-ins for pooled NGS readouts
  • HDR donors & landing pads for targeted integration​
    • ​

Gene Fragment Assembly Deliverables

Standard deliverables include 500-1,000 ng of dried double-stranded DNA, depending on sequence length.


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