The epigenome encompasses chemical modifications to DNA and histone proteins, chromatin accessibility, and higher-order DNA organization. Its profound effect on gene regulation, and consequently development and pathogenesis, is only beginning to be understood. Although epigenetic modifications do not alter the nucleic acid sequence itself, sequencing-based techniques can detect them indirectly.
As a leading provider of next generation sequencing (NGS), GENEWIZ offers a suite of powerful NGS solutions, including whole genome bisulfite sequencing (WGBS), to rapidly and thoroughly analyze the epigenome of many organisms.
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Whole genome bisulfite sequencing (WGBS) provides robust, global detection of 5-methylcytosine (5mC), a common form of DNA methylation in eukaryotes.
Whole genome sequencing on the PacBio® Sequel® can reveal a diverse array of DNA modifications, including 6-methyladenine (6mA) and 4-methylcytosine (4mC), as well as perform haplotype phasing of epigenetic markers.
Assay for transposase-accessible chromatin sequencing (ATAC-Seq) employs Tn5 transposase to identify open chromatin with a higher signal-to-noise ratio and lower input requirements than other approaches.