Epigenomics (Epigenetic Sequencing)

 

Epigenomics, also known as Epigenetic sequencing, utilizes next generation sequencing (NGS) assays to analyze the epigenome of many organisms rapidly and thoroughly.

The epigenome encompasses chemical modifications to DNA and histone proteins, chromatin accessibility, and higher-order DNA organization. Its profound effect on gene regulation, and consequently development and pathogenesis, is only beginning to be understood. Although epigenetic modifications do not alter the nucleic acid sequence itself, sequencing-based techniques can detect them indirectly.

As a leading provider of NGS, GENEWIZ Multiomics & Synthesis Solutions from Azenta Life Sciences offer a suite of powerful epigenomic sequencing solutions, including methylation sequencing and whole genome bisulfite sequencing (WGBS).

 

What is epigenomics?

Epigenomics is the study of reversible modifications in a cell’s DNA that regulate gene expression and mark the genome (turning the gene “on” or “off”) without altering the underlying DNA sequence itself. The field of epigenomics provides insight into complex cellular diseases and how cellular phenotypes are regulated by gene expression dynamics.

 

What is epigenetic sequencing?

Epigenetic sequencing is the use of high-throughput sequencing technology to quantify and analyze DNA modifications that are involved in gene expression and regulation of cell differentiation and development.

 

What are the methods of epigenetic sequencing?

To perform epigenetic sequencing, DNA methylation must be preserved and transformed into quantitative signals for genome-wide mapping. There are several methods of epigenetic sequencing; bisulfite sequencing (BS-Seq) and immunoprecipitation (methylated DNA immunoprecipitation or MeDIP) are commonly used in conjunction with NGS to identify epigenetically modified regions of the genome.

Epigenetic Sequencing Service Options

Methylation Sequencing


methylation sequencing provides robust, global detection of 5-methylcytosine (5mC), a common form of DNA methylation in eukaryotes.

PacBio® Methylation Sequencing


Whole genome sequencing on the PacBio® Sequel® can reveal a diverse array of DNA modifications, including 6-methyladenine (6mA) and 4-methylcytosine (4mC), as well as perform haplotype phasing of epigenetic markers.

ATAC-Seq


Assay for transposase-accessible chromatin sequencing (ATAC-Seq) employs Tn5 transposase to identify open chromatin with a higher signal-to-noise ratio and lower input requirements than other approaches.

ChIP-Seq


Chromatin immunoprecipitation sequencing (ChIP-Seq) identifies protein-DNA interactions and histone modifications across the genome, producing data with better resolution, less noise, and higher coverage than array-based technologies.

Single-Cell ATAC-Seq


Single-cell ATAC-Seq (scATAC-Seq) identifies genome-wide chromatin accessibility by profiling single cells in parallel, allowing researchers to examine how chromatin structure and DNA-binding proteins regulate gene expression in varying states and cellular processes.

How To Order


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