The table below provides guidance on selecting the appropriate cell line based on factors such as expression system, target protein, and downstream applications.
|Regular cloning and storage, uptake of large plasmids
|Regular cloning and storage
|lentiviral and retroviral plasmids, plasmids prone to rearrangement
|Proprietary cell strain used for our AAV-ITR plasmid production services, designed to maximize ITR stability
Fresher is better; < 3days old with well isolated colonies. Older plates can be freshly streaked for isolation upon request. Please note that this will add to the order timeline.
Please use caution in shipping agar plates during the cold winter months. The agar media may freeze in transit, rendering the plate unusable. Glycerol stocks are recommended as a substitute format during this season.
Larger plasmids (>10 kb) and plasmids containing toxic or unstable sequences (such as long terminal repeats (LTR) found in viral expression vectors) are likely to produce better results when paired with cell lines engineered to maintain these difficult sequences.
We advise against using standard cloning strains for these plasmids, due to increased risk of lower yield and plasmid instability. We also suggest the selection of additional QC testing to verify unstable or toxic plasmids.
Our internal quality monitoring will trigger the automatic repeat of a plasmid DNA preparation when the yield is below a critical threshold, depending on the anticipated copy number for the plasmid. Yield variance above this threshold could be due to a variety of factors and may be intrinsic to the sample provided.
Plasmid DNA preparation orders are processed by run. For low copy or other low yield plasmids, we recommend ordering a larger preparation scale or multiple preps, depending on the amount of final material required.
To discuss troubleshooting or custom process modifications, please contact Azenta Technical Support.