Gene Synthesis FAQs


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Backed by over 99.9% project completion rate, GENEWIZ can synthesize naturally occurring sequences, codon-optimized genes, gene libraries, complex sequences, large sequences, or any other sequence you need for your research - regardless of complexity.
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Single-stranded oligonucleotides are designed, synthesized, and assembled using different protocols depending on sequence characteristics. Subsequently, the assembled full-length gene is cloned into the pUC57 vector; the insert is verified by Sanger DNA sequencing and restriction digestion.
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GENEWIZ will synthesize cloned gene sequences ranging from 1 bp to 50+ kb, using short DNA oligos as building blocks.

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GENEWIZ has a proven track record of synthesizing difficult templates, such as GC-rich sequences and sequences with repeats, using proprietary technologies. With our bioinformatics platform, GENEWIZ optimizes your gene for synthesis. In some very rare instances, extreme sequence content may be refractory to available sequence verification techniques. GENEWIZ carefully reviews every sequence to estimate difficulty and anticipate project challenges, and customizes every project quotation to include an estimated turnaround time and pricing information based on this analysis.

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For genes up to 1.5Kb, the completion time is 8-10 business days. TurboGENE™ service is available with completion in as few as 5 business days.

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GENEWIZ provides a custom quote for every gene synthesis project. For standard priority, typical gene sequences (non-repetitive, average GC-content) that do not require custom subcloning, no additional fees apply. For genes with expedited handling, difficult sequences, large inserts, or for projects with custom subcloning requirements, any relevant fees will be indicated in your custom gene synthesis quote.

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GENEWIZ can deliver codon-optimized genes, designed to meet your exact research specifications. These sequences are always available for your review prior to confirming your order, typically included in the project quote.

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GENEWIZ codon optimization tool has the ability to optimize multiple critical parameters to stabilize DNA fragments and improve gene expression efficiency. Parameters include but are not limited to:

  • Codon Usage Bias;
  • GC content;
  • mRNA Secondary Structure;
  • Custom Desired Patterns;
  • Custom Undesired Patterns;
  • Repeat Sequences (direct repeat, inverted repeat, and dyad repeat);
  • Restriction Enzyme Recognition Sites (deletion or insertion).
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The assembled full-length gene is cloned into the pUC57 vector, and verified with Sanger DNA sequencing on at least one strand and restriction digestion.


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Standard cloning into pUC57 typically occurs via EcoRV, however the exact final sequence may vary. If you require specific restriction sites to flank your insert, please include them into your gene synthesis sequence to be synthesized.


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GENEWIZ offers both ampicillin and kanamycin marked version of pUC57, at no charge.


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Customers must supply vectors other than pUC57. If you need to clone your synthetic gene into a custom vector, please send GENEWIZ the vector DNA and pertinent information (i.e. vector size, sequence, cloning site, copy number, etc.).


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GENEWIZ conducts sequence verification during the gene synthesis process to ensure your synthesis product is as intended. Final plasmid constructs are clonally isolated and confirmed by Sanger DNA sequence analysis to at least single-strand depth across the synthesized insert. Purified PCR fragments are sequence verified on both strands, although verification at the fragment ends is limited to single strand depth. Subassemblies and intermediate products may be cloned and verified to contain the correct sequence, as needed. DNA sequence re-verification after scale-up DNA preparation is available as an added option to your gene synthesis order.

On completion of your synthetic gene, GENEWIZ delivers an electronic data package in parallel with your construct. The data package includes the original sequencing data files from construct verification to facilitate your independent review.

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Standard deliverables for GENEWIZ gene synthesis projects include:

  • 2-5 µg of lyophilized plasmid containing your desired synthetic construct;
  • Certificate of Analysis (COA), including restriction digest;
  • Sequence trace data with alignment;
  • Sequence of synthetic gene alone and in the designated vector.
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GENEWIZ sample tubes contain 2-5 µg lyophilized plasmid DNA, or 1-2 µg purified PCR fragment DNA for standard synthesis projects. For resuspension, we recommend:

  • Spin the tube briefly to ensure that the contents are on the bottom of the tube;
  • Resuspend the sample in a volume appropriate for your needs:
    • For example, 20-50 µL for ~0.25 to ~0.1 µg/µL of sterile high purity water or TE;
    • The resuspension solution is dependent on downstream applications - TE may interfere with various enzyme manipulations;
  • Vortex briefly, let the DNA tube sit for 2-10 minutes on ice, then vortex again.
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GENEWIZ is fully aware of all risks and benefits associated with gene synthesis research. With the knowledge that gene synthesis technologies have the ability to enable de novo reconstruction of dangerous pathogens, GENEWIZ employs rigorous quality control policies and procedures to safeguard against abuse of the genes we synthesize. GENEWIZ actively monitors the Screening Framework Guidance for Synthetic Double-Stranded DNA Providers, drafted by U.S. Department of Health and Human Services (HHS), as well as the Select Agents and Toxins list put forth by the U.S. Government.
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At GENEWIZ, protection of our customers' intellectual property is a top priority. Proprietary information is always under client ownership, and GENEWIZ places the utmost importance on confidentiality and privacy. Comprehensive protective measures enable GENEWIZ to earn and maintain the trust and confidence of all customers, collaborators, and partners. For more information about the GENEWIZ Confidentiality Policy, please click here.

How to Order Gene Synthesis


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  • To request a gene synthesis quote, please click here to login to your GENEWIZ account.
  • Under the heading, "Place an Order," click "Create Gene Synthesis Quote Request."
  • Select the desired Gene Synthesis service priority, and then click "Save & Next."
  • To submit your Gene Synthesis quote request, please complete the online quote request form.

For standard priority services, a Gene Synthesis Uploadable Quote Request Form is also available.


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  • To request a bulk gene synthesis quote, please click here to login to your GENEWIZ account.
  • Under the heading, "Place an Order," click "Create Gene Synthesis Quote Request."
  • Select standard service priority, and "Create Quote By" Excel Form.

The GENEWIZ Gene Synthesis Quote Request Excel form upload function enables submission of up to 500 sequences per form. Click here to learn more about the Gene Synthesis Uploadable Quote Request Form. 



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  • To request a TurboGENE™  quote, please click here to login to your GENEWIZ account.
  • Under the heading, "Place an Order," click "Create Gene Synthesis Quote Request."
  • Select the TurboGENE™  5-Day or TurboGENE™  7-Day for service priority, and then click "Save & Next."
  • To submit your TurboGENE™  quote request, please complete the online quote request form.

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GENEWIZ Project Management is composed of specialized experts dedicated to your project's success. Project Management is available to assist with any questions throughout the duration of your gene synthesis project. In addition, GENEWIZ Project Management will provide milestone updates at each stage of your gene synthesis process, keeping you well-informed throughout all stages of research.

To contact GENEWIZ Project Management, please click here.

Have a specific question?

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