TARGETED SEQUENCING

Targeted Sequencing is an effective method for analyzing your selected gene(s) of interest by next generation sequencing. Whether you are interested in a few genes, or a few hundred genes, targeted resequencing panels offer high sensitivity and specificity, providing in-depth coverage, resulting in high-quality data.

With Custom Targeted Sequencing gene panels, you can discover point mutations, insertions/deletions (INDELs), copy number variations (CNVs), and gene rearrangements.

Targeted sequencing Gene Panels

GENEWIZ provides a variety of targeted resequencing panels, either pre-made or custom, that can cover any genes of interest or cancer-implicated genes.

Visit these pages to learn more about these services:

GENEWIZ also offers Sanger-based confirmatory sequencing to verify your final genes of interest.

Applications

Discover genetic biomarkers associated with disease state

Characterize genetic associations to a particular phenotype, such as drug response

Genotype and classify eukaryotic and/or prokaryotic cell lines

Design genetic tests, such as for pharmacogenomics or oncology

Features & Benefits

Extensive multiplexing flexibility enables a more cost-effective assay

Dedicated, on-going support from experienced GENEWIZ scientists

In-house pipeline for optional Sanger-based confirmatory sequencing of final targets utilizing your NGS samples

Comprehensive bioinformatics options for robust downstream analysis

Availability of the most cutting-edge sequencing platforms

Workflow for Targeted Resequencing Panels

Extensive Quality Control is Performed at the Conclusion of Each Step of the Process

Experimental Design

1
Targeting and Library Preparation

2
Sequencing

3
Bioinformatics

4

Sample Submission Guidelines

GENEWIZ accepts the following sample types for targeted resequencing projects:

Purified genomic DNA
Frozen cell pellets (mammalian or bacterial)
PCR amplicons
Bacterial colonies

Formalin-fixed, paraffin-embedded (FFPE) thin sections (slides)
Select tissues
Blood
Swab

Technical Resources

Blog | NGS, qPCR, or Sanger Sequencing: An Assay Selection Guide

This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.