Targeted sequencing uses next generation sequencing (NGS) to analyze your selected gene(s) of interest. Whether you are interested in a few genes or a few hundred genes, targeted sequencing panels offer high sensitivity and specificity, providing in-depth coverage, resulting in high-quality data.
With custom targeted sequencing gene panels, you can discover point mutations, insertions/deletions (INDELs), copy number variations (CNVs), and gene rearrangements.
Targeted sequencing is a technique that uses NGS to target specific genes, coding regions of the genome, or chromosomal segments for rapid identification and analysis of genetic mutations.
Targeted sequencing is useful for studying gene variants in selected genes or specific regions of the genome, since a large diversity of organisms is targeted rapidly and cost-effectively. Targeted sequencing is used to examine gene interactions in specific pathways and is faster and more cost effective than whole genome sequencing (WGS). This is because a smaller set of nucleotides are used, rather than broadly sequencing the entire genome.
Azenta accepts the following sample types for targeted resequencing projects:
This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.
Developing immunotherapies for cancer can be difficult due to the variation of immune response from patient to patient. In this webinar, Dr. Litchfield from UCL Cancer Institute presents his team’s exploratory research using a multiomics approach to better understand the diversity of immune response to cancer and highlights their findings of an alternative source of a tumour-specific antigen in checkpoint inhibitor (CPI) response.
In this co-hosted webinar with Twist Bioscience, learn how researchers design custom panels to sequence deeply and efficiently into regions, genes, and organisms of interest, as well as gain a greater understanding on how targeted sequencing can be combined with the latest transcriptomics and proteomics tools to reveal even more insights.
Azenta is a certified service provider for Illumina NGS platforms and the PacBio Sequel. Please visit our NGS Platforms page for more information.