TARGETED SEQUENCING

Targeted Sequencing is an effective method for analyzing your selected gene(s) of interest by next generation sequencing. Whether you are interested in a few genes, or a few hundred genes, targeted resequencing panels offer high sensitivity and specificity, providing in-depth coverage, resulting in high-quality data.

With Custom Targeted Sequencing gene panels, you can discover point mutations, insertions/deletions (INDELs), copy number variations (CNVs), and gene rearrangements.

Targeted sequencing Gene Panels

Azenta provides a variety of targeted resequencing panels, either pre-made or custom, that can cover any genes of interest or cancer-implicated genes.

Visit these pages to learn more about these services:

Azenta also offers Sanger-based confirmatory sequencing to verify your final genes of interest.

Applications

Discover genetic biomarkers associated with disease state

Characterize genetic associations to a particular phenotype, such as drug response

Genotype and classify eukaryotic and/or prokaryotic cell lines

Design genetic tests, such as for pharmacogenomics or oncology

Features & Benefits

Extensive multiplexing flexibility enables a more cost-effective assay

Dedicated, on-going support from experienced Azenta scientists

In-house pipeline for optional Sanger-based confirmatory sequencing of final targets utilizing your NGS samples

Comprehensive bioinformatics options for robust downstream analysis

Availability of the most cutting-edge sequencing platforms

Workflow for Targeted Resequencing Panels

Extensive Quality Control is Performed at the Conclusion of Each Step of the Process

Experimental Design

1
Targeting and Library Preparation

2
Sequencing

3
Bioinformatics

4

Sample Submission Guidelines

Azenta accepts the following sample types for targeted resequencing projects:

Purified genomic DNA
Frozen cell pellets (mammalian or bacterial)
PCR amplicons
Bacterial colonies

Formalin-fixed, paraffin-embedded (FFPE) thin sections (slides)
Select tissues
Blood
Swab

Technical Resources

Blog | NGS, qPCR, or Sanger Sequencing: An Assay Selection Guide

This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.

Webinar| Non-Classical Sources of Tumour-Specific Antigen in Checkpoint Inhibitor Response

Developing immunotherapies for cancer can be difficult due to the variation of immune response from patient to patient. In this webinar, Dr. Litchfield from UCL Cancer Institute presents his team’s exploratory research using a multiomics approach to better understand the diversity of immune response to cancer and highlights their findings of an alternative source of a tumour-specific antigen in checkpoint inhibitor (CPI) response.