Avoid repeats and save time and money by utilizing GENEWIZ's alternative protocol for difficult templates. Difficult templates can be challenging to sequence due to secondary structures, large size, high GC content, and other inherent factors. While the standard DNA sequencing may produce satisfactory results for borderline samples, GENEWIZ's proprietary alternative protocols perform best when sequencing difficult templates.
Template examples that may benefit from the alternative protocols include:
The small hairpin RNA templates used in RNAi studies frequently present challenges in sequencing such as early termination or ambiguity, caused by the double-stranded or hairpin structures. Our proprietary RNAi Protocol produces superior results for our customers, saving time and cost. For a comparison, see the chromatogram below:
Repeat/GC-rich templates have special secondary structures and melting temperatures. DNA containing high GC content and hairpin structures are relatively more difficult to denature and very challenging to sequence.
Our proprietary Hairpin and GC-rich protocols produce superior results, with strong signals and uniform peak heights at the GC region.
Large constructs such as Bacterial Artificial Chromosomes (BACs), cosmids, fosmids, and BACmids, pose a challenge to traditional Sanger sequencing protocols due to the size and interference of the non-target DNA in the template. GENEWIZ's proprietary BAC protocol to overcome the difficulty of sequencing directly from these large constructs can help deliver greater success and longer read lengths for large constructs compared to the standard protocol.
Please contact our Technical Support team for more information on BAC protocol's:
*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.