Difficult Template sequencing is a proprietary Sanger protocol adept at tackling GC-rich regions, hairpins, and intricate secondary structures, ensuring a success rate more than double that of standard workflows. With GENEWIZ Difficult Template sequencing from Azenta Life Sciences, save at least 1 day in the sequencing process, and streamline your research.
Compatible with various DNA types including PCR products, plasmids, bacterial colonies, and glycerol stocks, our service eliminates the need for additional templates, providing unparalleled convenience and efficiency for your breakthrough projects.
Template examples that may benefit from the alternative protocols include:
The small hairpin RNA templates used in RNAi studies frequently present challenges in sequencing such as early termination or ambiguity, caused by the double-stranded or hairpin structures. Our proprietary RNAi Protocol produces superior results for our customers, saving time and cost. For a comparison, see the chromatogram below:
Repeat/GC-rich templates have special secondary structures and melting temperatures. DNA containing high GC content and hairpin structures are relatively more difficult to denature and very challenging to sequence.
Our proprietary Hairpin and GC-rich protocols produce superior results, with strong signals and uniform peak heights at the GC region.