AMPLICON SEQUENCING SERVICES

 

NGS-based amplicon sequencing allows detection of low frequency variants, quantitative analysis of mixed populations, and scalable analysis of a large number of samples. Whether you are looking to validate/discover variations in specific genomic regions or are interested in screening clones, GENEWIZ offers multiple options for ultra-deep sequencing of amplicons (PCR products). GENEWIZ employs the latest technologies to provide custom, in-depth solutions for amplicons ranging from 100 base pairs (bp) to >10,000 bp.

Applications for this service include antibody library screening, natural antibody repertoire profiling, disease genotyping, CRISPR mutation analysis, 16/18S gene sequencing, screening of molecular clones, and analysis of plasmid stocks.

 

NGS Amplicon Services

Multiple Solutions to Answer Every Biological Question

Amplicon-EZ

Illumina based

50K reads/sample guaranteed. Available for amplicons 150-500 bp in size.

Standard Amplicon Sequencing

Illumina based

Flexible data quantity options. Available for amplicons >100 bp.

Ultra-Long Amplicon Sequencing

PacBio Sequel

Contiguous sequencing of amplicons up to 10 kb.

    GENEWIZ's Recommendation
 Amplicon Size Applications Library Preparation Type Platform Configuration 
100-250 bp
  • SNP Genotyping
  • Genome editing clone verification
  • Somatic/complex variant discovery

Adapter ligation to
full-length products

Illumina 2x150 bp
 250-550 bp
  • Antibody heavy or light chain
  • Hypervariable 16S
Adapter ligation to
full-length products
Illumina 2x250 bp
or
2x300 bp
  • Somatic variant analysis 
Fragmentation Illumina 2x150 bp
>550 bp
  • Paired antibody heavy and light chains
  • Full length 16S
  • Variant phasing
Adapter ligation to
full-length products
PacBio Sequel
  •  Somatic variant analysis
Fragmentation Illumina 2x150 bp

Features & Benefits

Optimized processes- exceed manufacturer’s specifications for data amount and quality
Results in as few as three business days
Multiplexing flexibility enables a more cost-effective assay
Latest long-read technologies
Automation reduces sample handling errors and increases reproducibility
Interactive analysis reports and free post-delivery support

Sample Submission Guidelines

GENEWIZ accepts purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids. For detailed sample submission requirements please visit our Sample Submission Guidelines.

 

Deliverables

All customers receive their raw data as FASTQ files. Customizable data analysis packages are available by request.

How To Order


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 1) | Live Chat