NGS-based amplicon sequencing allows detection of low frequency variants, quantitative analysis of mixed populations, and scalable analysis of a large number of samples. Whether you are looking to validate/discover variations in specific genomic regions or are interested in screening clones, Azenta Life Sciences, formerly GENEWIZ offers multiple options for ultra-deep sequencing of amplicons (PCR products). Azenta employs the latest technologies to provide custom, in-depth solutions for amplicons ranging from 100 base pairs (bp) to >10,000 bp.

Applications for this service include antibody library screening, natural antibody repertoire profiling, disease genotyping, CRISPR mutation analysis, 16/18S gene sequencing, screening of molecular clones, and analysis of plasmid stocks.




Whole plasmid sequencing in as fast as 1 business day upon receipt in processing lab


NGS Amplicon Services

Multiple Solutions to Answer Every Biological Question



50K reads/sample. Available for amplicons 150-500 bp in size.

Custom Short-Read Amplicon Sequencing


Flexible data quantity options. Available for amplicons >100 bp.

Long-Read Amplicon Sequencing

PacBio® Sequel®

Contiguous sequencing of amplicons up to 10 kb.

    Azenta's Recommendation
 Amplicon Size Applications Library Preparation Type Platform Configuration 
100-250 bp
  • SNP Genotyping
  • Genome editing clone verification
  • Somatic/complex variant discovery

Adapter ligation to
full-length products

Illumina 2x150 bp
 250-550 bp
  • Antibody heavy or light chain
  • Hypervariable 16S
Adapter ligation to
full-length products
Illumina 2x250 bp
2x300 bp
  • Somatic variant analysis 
Fragmentation Illumina 2x150 bp
>550 bp
  • Paired antibody heavy and light chains
  • Full length 16S
  • Variant phasing
Adapter ligation to
full-length products
PacBio Sequel
  •  Somatic variant analysis
Fragmentation Illumina 2x150 bp

Features & Benefits

Optimized processes- exceed manufacturer’s specifications for data amount and quality
Results in as few as 8 business days depending upon project type
Multiplexing flexibility enables a more cost-effective assay
Latest long-read technologies
Automation reduces sample handling errors and increases reproducibility
Interactive analysis reports and free post-delivery support

Technical Resources

Case Study | Developing an Inclusion and Safety Monitoring Assay for a Cell Therapy Clinical Trial

A biopharma company required a partner for clinical trial support, with an immediate need for development and validation of a biomarker assay using next generation sequencing with a quick turnaround time. Read the case study to learn how this company was able to streamline clinical testing and accelerate the timeline of its phase 1/2 study.

Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide

This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.

Sample Submission Guidelines

Azenta accepts purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids. For detailed sample submission requirements please visit our Sample Submission Guidelines.



All customers receive their raw data as FASTQ files. Customizable data analysis packages are available by request.

How To Order

Email | Phone 1-877-436-3949, Ext. 1