CRISPR VALIDATION

Recent advancements in genome editing techniques, namely CRISPR/Cas9, have greatly facilitated cell line engineering, gene targeting, and the development of transgenic models. However, identification of correctly targeted cells remains a significant bottleneck for large-scale projects.

To remove this hurdle, Azenta Life Sciences, formerly GENEWIZ developed genoTYPER-NEXT™, a proprietary NGS-based assay ideal for ultra-sensitive, high-throughput, sample-to-answer genotyping projects. This tool is more effective and efficient than T7E1, TIDE, and IDAA assays for screening targeted cell lines.

We also offer targeted, whole genome, and amplicon sequencing NGS solutions to aid on- and off-target analysis of genome editing.

genoTYPER-NEXT: HIGH-THROUGHPUT ASSAY FOR CRISPR VALIDATION

1. SUBMIT SAMPLES

Submit CRISPR-edited cell lines in 96-well plates

2. AMPLIFY ON/OFF-TARGET SITE(S)

Cells lysis and PCR with barcoded primers

3. SEQUENCE

Samples pooled and sequenced on Illumina® platform

4. ANALYZE DATA INTERACTIVELY

Visualize results (login required) in genoTYPER-NEXT browser

ADDITIONAL NGS SERVICES FOR CRISPR VALIDATION

FEATURES & BENEFITS

Automated, high-throughput workflow – Up to 10,000 samples per run

Hassle-free - Avoid gDNA extraction, pre-screening prior to sequencing, and TA cloning

Highly sensitive – Detection of <1% allele frequency, full INDEL resolution, and frameshift analysis

More reasons to choose Azenta

Technical Resources

Case Study | Rapid, Scalable, and Ultra-Sensitive Validation of Allele-Specific Gene Editing Events with genoTYPER-NEXT™

Discover the powerful capabilities of genoTYPER-NEXT in this illuminating case study and learn how this high-throughput assay allows rapid and accurate genotyping of CRISPR-edited cell lines.

Webinar | Rapid and Sensitive Validation of Gene Editing with Novel NGS Assays

Learn about current techniques to validate gene edits and how recent advancements in next generation sequencing are improving the sensitivity and scalability of screening genomic manipulations.

Blog | NGS, qPCR, or Sanger Sequencing: An Assay Selection Guide

This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.