CRISPR Construct Synthesis

CRISPR/Cas9 and other CRISPR-associated systems have revolutionized the field of genome engineering.  CRISPR-mediated genome editing makes highly flexible, highly specific, and affordable genome engineering possible. This powerful system allows for almost any genomic target to be modified in any organism that can express exogenous DNA.   Have your pre-designed constructs that are ready for synthesis?  GENEWIZ can synthesize the CRISPR sequence, clone it into your vector of choice, and perform sequence verification.
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Limited Time Offer!
Synthesize your CRISPR gRNA constructs for only $69

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Your Design. Our Synthesis.

GENEWIZ offers very competitive pricing to synthesize, clone and sequence verify your gRNA expression cassettes into your preferred vector regardless of vector size or complexity.  Our strong expertise and experience in highly reliable synthetic construct generation services coupled with our CRISPR offering makes us a valuable partner to researchers around the globe. Simply provide your pre-design sequence to GENEWIZ for any of the CRISPR-related products below and receive a quote within one business day:
 
gRNA Synthesis and Cloning  
GENEWIZ will synthesize and clone your gRNA sequences as cDNA into your preferred vector regardless of vector size and complexity, using the same industry-leading proprietary technology that GENEWIZ has developed for standard gene synthesis products.
 
Homology Directed Repair (HDR) Donor Construct and Custom Vector Synthesis
Need a custom donor or vector construct synthesized? GENEWIZ has years of experience working with complex and unstable vectors.  We can provide custom constructs for your HR-mediated repair template as well as custom CRISPR vector generation and modifications. All custom vectors can be stored in-house for your future orders for up to 2 years at no additional cost.
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Standard Deliverables:
- 2-5 µg of lyophilized plasmid containing your sequence verified synthetic construct
- Certificate of Analysis (COA) including restriction digest
- Sequence trace data with alignment
- Sequence files of synthetic gene alone and in vector
 
Synthetic Libraries
Looking to create a gRNA library to screen knock-outs/knock-ins of a variety of genes in one experiment? GENEWIZ custom synthetic library service can create a custom gRNA library for your high-throughput CRISPR screening needs.

 

 

Typical GEne Synthesis Workflow

  • Oligonucleotide Synthesis


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  • Gene Assembly


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  • Cloning


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  • Quality Control


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  • Synthetic Gene Shipment


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HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.


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