Digital Spatial Profiling

 

Digital spatial profiling allows you to view your tissue in a new light by combining high-plex gene expression profiling with the spatial resolution of immunohistochemistry. This technology is useful for examining cellular interactions, tissue heterogeneity, pathogenicity, and response to therapy.

GENEWIZ is proud to be one of the first service providers to offer digital spatial profiling utilizing the NanoString® GeoMx™ platform. This novel technology performs simultaneous in situ spatial analysis of over 40 proteins or 100 mRNAs from a single FFPE or frozen tissue section. Together with our epigenetic and single-cell solutions, GENEWIZ equips researchers with the most cutting-edge technologies to study gene expression from every angle.


  

MORPHOLOGICALLY-GUIDED GENE EXPRESSION PROFILING

GENEWIZ’s digital spatial profiling service on the GeoMx platform enables morphologically-guided gene expression profiling of FFPE or fresh frozen tissues using one of NanoString’s expertly-curated, predesigned panels. Researchers can provide tissue blocks or slides for processing, or GENEWIZ can leverage its network of biobank partners to source high-quality, consented samples on your behalf.

Digital Spatial Profiling Stains

1. Stain Sections

Tissue is stained using a protein or mRNA panel and immunofluorescent markers

Digital Spatial Profiling Target Areas

2. Define Target Areas

Regions of interest (ROIs) are selected using the GeoMx software (see FAQs)

Photocleave Barcodes

3. Photocleave Barcodes

Barcodes are cleaved using UV light and captured

Separate ROIs

4. Separate ROIs

Barcodes from each ROI are deposited in separate tubes

Analyze Spatial Expression

5. Analyze Spatial Expression

ROI-specific barcodes are quantified to create expression profiles

APPLICATIONS OF DIGITAL SPATIAL PROFILING

Oncology

Oncology

 

  • Profile tumor heterogeneity, microenvironment, and cancer stem cells
  • Identify tumor-infiltrating lymphocytes (TILs)

 

Immunology

Immunology

 

  • In situ immune repertoire characterization
  • Immune response profiling of normal and diseased states

 

Neuroscience

Neuroscience

 

  • Contextually profile neural and stem cells
  • Characterization of neurodegenerative disease pathology

 

Pathology

Pathology

 

  • High-plex analysis of expression in normal and diseased tissues
  • Biomarker discovery and characterization
  • Monitor therapy response

 

Features & Benefits

Multiomic Solutions – Choose from expert-curated protein and mRNA panels targeting various pathways, or create your own

High-Plex Analysis – Combines the benefits of IHC/FISH and high-throughput expression analysis into one streamlined, tissue multiplexing assay

Target Multiple ROIs – Explore several ROIs from a single tissue slide using flexible manual and automatic ROI selection options

Preserve Samples – Multiple ROIs can be selected from a single slide, allowing tissue to remain intact for re-staining or use in other applications

More reasons to choose GENEWIZ

Technical Resources

High-Plex Spatial Analysis of Tissue Microenvironments

Webinar | Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments

In this webinar presented by Douglas Hinerfeld, Ph.D., Director of Product Applications at NanoString, learn how their novel GeoMx platform simultaneously analyzes dozens of proteins and transcripts with high spatial resolution, providing greater insight into biological systems and diseases across a variety of research areas.

Related Services

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