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  Sample Submission Guidelines

Sanger Sequencing


Sanger Sequencing is a cost-effective method for determining the nucleotide sequence of DNA. GENEWIZ Sanger sequencing services are award-winning, providing high-quality results, industry-leading customer service and fast turnaround times at competitive prices. GENEWIZ from Azenta Life Sciences is the partner of choice for academic, pharmaceutical, GLP, biotechnology, and government institutions around the world.

We are well-equipped to handle DNA sequencing of all complexities including single tube, high-throughput, direct bacterial colony, glycerol stock, CLIA, or GLP DNA sequencing projects. Our mission is to eliminate bottlenecks, improve productivity, and advance your research progress.

GENEWIZ Sanger Sequencing Service

Azenta accepts a variety of different templates to use as starting material for Sanger sequencing reactions.
Click on the service below to view the service options available for each starting material.


Standalone Sanger sequencing services are also offered at the GLP-compliant level. For more information, please email

Sanger Sequencing Features & Benefits

High Quality - DNA sequencing read lengths up to ~1000 bases.A typical read will provide 800 bases Phred20.  Quality Score and Contiguous Read Length (CRL) provided in each read.
Azenta Technical Support - Our highly-trained team of Sanger sequencing specialists proactively communicate updates, while providing technical expertise on projects big and small.
Convenient - Contact us to locate a drop box or pick-up service near you for free shipping.
Easy, Online Ordering - 24/7 web-based online ordering and data retrieval, with bar-coded order tracking make it easy to place your order and view data.
Best-in-Class Difficult Template Protocols - Our proprietary technologies allow for consisent, reliable data even on the most difficult templates (including hairpins and GC-rich DNA).
Free Vector Primers – a wide selection of Azenta Universal Primers are available for most standard vectors. Also visit our Universal Primer Selection Tool to determine which Universal Primer binds to your vector of choice.

Sanger Sequencing Protocol

* Samples must arrive at the Azenta New Jersey laboratory before 10:00 am EST to qualify for Same Day service.
Note that direct-sequencing templates are not available for our Same Day service.

Same Day Turnaround Time

Same Day

FOR RUSH PROJECTS: Data is reported by 7PM Eastern the same day we receive samples. A small additional charge applies. Please contact Technical Support for additional information.

Overnight Local Service Turnaround Time

Overnight Local Service

Overnight service for customers near a Azenta laboratory. Laboratories are located in New York City, Boston, Washington D.C. Metro Area, Research Triangle Park, San Diego, Los Angeles Metro Area, San Francisco Bay Area, and Seattle. Typically samples are picked up in the afternoon by a Azenta courier, and data are reported the next morning. Please contact us for details and eligibility.

Standard 24-Hour Turnaround Time

Standard 24-Hour

Data reported within one business day after samples are received by Azenta.

Troubleshooting DNA Templates
with Sanger Sequencing



*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.

Email | Phone (1-877-436-3949, Ext. 2)