Plasmid Preparation

Sample Submission Guidelines

Sample Type Sample Guideline
Clonal Plasmid DNA 5 µl at 200 ng/µl (minimum 100 ng)
Colonies on agar <3 days old; single and well isolated
Clonal Agar stab samples agar stabs
Clonal Glycerol stocks 200 µl of frozen stock
Frozen bacterial cell pellets varies by scale


Viral Plasmid Containing Inverted Terminal Repeat (ITR)

Be aware that sequencing ITR is challenging, so we request concentrated stocks of DNA.

  • Optimal sequencing concentration for Viral Plasmid is 300 ng/µl (minimum 200 ng/µl). Please submit as much template as you are able, up to 10 µl but no less than 5 µl.
  • Please include exact concentration in Notes field for each template.
  • Ensure 260/230 ration of 1.8-2.2, as this protocol is particularly sensitive to organic contamination.
  • Primer – Please provide 30 µl per reaction at 5 pmol/µl (5 µM) in a labeled 1.5 or 0.5 ml tube.
  • Generate primers that bind between 150 bps and 350 bp from ITR.

 

Contact

Azenta Life Sciences offers GENEWIZ DNA prep services at several of our labs, including San Francisco, New Jersey, Boston & San Diego.

GENEWIZ South San Francisco
201 Haskins Way, Suite 100
South San Francisco, CA 94080
Tel: 510-705-1455
12pm-8pm

GENEWIZ Global Headquarters
111 Corporate Boulevard
South Plainfield, NJ 07080
Tel: 908-222-0711
8am-8pm

GENEWIZ Waltham
1432 Main Street, Suite #110
Waltham, MA 02451
Tel: 617-300-0184
10am-7pm

GENEWIZ La Jolla
11099 North Torrey Pines Road, Suite 270
La Jolla, CA 92037
10am-8pm (M-F)

Direct Shipping (FedEx, UPS): 

If a dropbox is not available or if your starting material requires dry ice, please use an overnight delivery service such as FedEx or UPS. A printout of the online Order Summary page must be included with the material. 

All direct shipments should be sent to:

GENEWIZ MB
115 Corporate Boulevard 
South Plainfield, NJ 07080 
USA
908-222-0711

GENEWIZ Dropbox (if available):

Starting material that is stable at room temperature may be submitted via a GENEWIZ Dropbox where available. A printout of the online Order Summary page must be included with the material. Contact dnaseq@genewiz.com to request a dropbox pickup for your location or if you have any dropbox questions.