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Next Generation Sequencing
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Plasmid DNA Preparation
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AAV Plasmid PreparationStandard Plasmid DNA PrepTargeted Yield Plasmid DNA Prep
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Sanger Sequencing
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Gene Synthesis
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PreClinical and Clinical Services
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Plasmid Preparation
Sample Submission Guidelines
| Sample Type | Sample Guideline |
| Clonal Plasmid DNA | 5 µl at 200 ng/µl (minimum 100 ng) |
| Colonies on agar | <3 days old; single and well isolated |
| Clonal Agar stab samples | agar stabs |
| Clonal Glycerol stocks | 200 µl of frozen stock |
| Frozen bacterial cell pellets | varies by scale |
Viral Plasmid containing Inverted Terminal Repeat (ITR)
Be aware that sequencing ITR is challenging, so we request concentrated stocks of DNA.
- Optimal sequencing concentration for Viral Plasmid is 300ng/µl (minimum 200ng/µl). Please submit as much template as you are able up to 10 µl; no less than 5µl.
- Please include exact concentration in Notes field for each template.
- Ensure 260/230 ration of 1.8-2.2, as this protocol is particularly sensitive to organic contamination.
- Primer – Please provide 30 µl per reaction at 5 pmol/µl (5 µM) in a labeled 1.5 or 0.5 ml tube.
- Generate primers that bind between 150bps and 350bp from ITR.
Contact
GENEWIZ offers DNA prep services at serveral of our labs, including San Francisco, New Jersey, Boston & San Diego .
GENEWIZ San Francisco
4000 Shoreline Ct, Suite 400
South San Francisco, CA 94080
Tel: 510-705-1455
12pm-8pm
GENEWIZ Global Headquarters
115 Corporate Boulevard
South Plainfield, NJ 07080
Tel: 908-222-0711
8am-8pm
GENEWIZ Boston
733 Concord Ave.
Cambridge, MA 02138
Tel: 617-300-0184
10am-7pm
11099 North Torrey Pines Road, Suite 270
La Jolla, CA 92037
10am-8pm (M-F)
Direct Shipping (FedEx, UPS):
If a Drop Box is not available, or if your starting material requires dry ice, please use an overnight delivery service such as FedEx or UPS. A print out of the online “order summary” page must be included with the material.
All direct shipments should be sent to:
GENEWIZ MB
115 Corporate Boulevard
South Plainfield, NJ 07080
USA
908-222-0711
GENEWIZ Drop Box (if available):
Starting material that is stable at room temperature may be submitted via a GENEWIZ Drop Box (when available). A print out of the online “order summary” page must be included with the material. Contact dnaseq@genewiz.com to request a Drop Box pick-up for your location or if you have any Drop Box questions.