SINGLE-CELL sequencing workflow
1. Sample Submission
Sample types include live, cryopreserved and fixed: isolated cells, cultured cells, primary tissue, organoids and more.
2. Sample QC and Cell Partitioning
Assessment of cell counting, viability testing and dead cell removal, when indicated, maximizes sample quality prior to loading. Cells and barcoded beads are then isolated in droplets using the 10x Genomics Chromium X.
3. Library Preparation
Unique barcodes for each individual cell are added to RNA or DNA prior to library preparation. Short-read and long-read library preparation options are available.
Barcoded libraries are pooled and sequenced on the Illumina NovaSeq X Plus or PacBio Revio system, as indicated by library preparation, for high-throughput characterization.
5. Data Analysis
Receive custom analysis and visualize results through interactive software. Complimentary Ph.D.-level support is available post-delivery to help drive biological insights.