Cell populations are seldom homogeneous and similar in their characteristics. While traditional bulk sequencing methods can be powerful, cost-effective tools for analysis, they cannot detect differences that arise between individual cells in complex environments. With recent developments in microfluidics and molecular barcoding, however, NGS-based technologies for genomic, transcriptomic, and epigenomic research have increasingly focused on the analysis of single cells, enabling scientists to examine the diversity of heterogenous cell populations and uncover new biological discoveries.
GENEWIZ offers a suite of end-to-end single-cell sequencing solutions utilizing the latest technology on the market: the 10x Genomics® Chromium™ and Illumina® NovaSeq™. As an early adopter of these platforms, our optimized workflows — including pre-submission cryopreservation and post-submission dead cell removal — maximize project flexibility, speed, and data accuracy.
Analyze transcriptome heterogeneity at the single-cell level. GENEWIZ offers optimized workflows for 5’ and 3’ gene expression libraries to uncover cellular differences that are masked by bulk RNA sequencing.
Cells and barcoded beads are isolated in droplets using the 10x Genomics Chromium
Unique barcodes for each individual cell are added to RNA or DNA prior to library preparation
Barcoded libraries are pooled and sequenced on the Illumina platform for high-throughput characterization
Visualize results through interactive software and receive custom analysis
Exceeding manufacturer’s benchmarks
Through our online system
At every step
Single-cell analysis typically relies upon immediate processing of fresh tissue, presenting limitations for translational and clinical applications. Learn how GENEWIZ’s robust cryopreservation workflow for downstream processing enabled high-quality, single-cell transcriptomic and epigenomic analysis of translational and clinical samples by coupling scATAC-seq and scRNA-seq.
Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.
In this webinar, we review how recent advancements of the 10x Genomics Chromium™ system, coupled with GENEWIZ developmental efforts, can benefit the researcher. In addition, we spotlight the new Single-Cell V(D)J Solution, which can be used in conjunction with single-cell transcriptome profiling to identify pairing of full-length BCR and TCR sequences
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With several commercial platforms available, high-throughput single-cell sequencing is now more accessible than ever. In this article, we’ll touch upon the 3 most important factors to consider before embarking on your single-cell project to ensure you select the right NGS approach for your research.
In comparison to traditional profiling methods which assess bulk populations, single-cell technologies empower researchers to examine diversity of heterogeneous cell populations and uncover new, and potentially unexpected, biological discoveries. This webinar highlights the unparalleled capabilities of single-cell sequencing.
GENEWIZ is a certified service provider for Illumina and Life Technology NGS platforms. For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
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