Cell populations are seldom homogeneous and similar in their characteristics. While traditional bulk sequencing methods can be powerful, cost-effective tools for analysis, they cannot detect differences that arise between individual cells in complex environments. With recent developments in microfluidics and molecular barcoding, however, NGS-based technologies for genomic, transcriptomic, and epigenomic research have increasingly focused on the analysis of single cells, enabling scientists to examine the diversity of heterogenous cell populations and uncover new biological discoveries.
GENEWIZ offers a suite of end-to-end single-cell sequencing solutions utilizing the latest technology on the market: the 10x Genomics®Chromium™ and Illumina® NovaSeq™. As an early adopter of these platforms, our optimized workflows — including pre-submission cryopreservation and post-submission dead cell removal — maximize project flexibility, speed, and data accuracy.
Single-Cell RNA-Seq
Analyze transcriptome heterogeneity at the single-cell level. GENEWIZ offers optimized workflows for 5’ and 3’ gene expression libraries to uncover cellular differences that are masked by bulk RNA sequencing.
Single-Cell ATAC-Seq
Identify chromatin accessibility in the epigenome by profiling thousands of single cells in parallel, and discover how chromatin structure and DNA-binding proteins regulate gene expression in varying states and cellular processes.
Single-Cell Immuno-Profiling
Examine the immune repertoires of individual B and T cells by physically pairing VH/VL or α/β chains in B- or T-cell receptors, respectively, while simultaneously capturing data for gene expression, V(D)J clone types, and cell surface protein expression.
Cells and barcoded beads are isolated in droplets using the 10x Genomics Chromium
2. Library Preparation
Unique barcodes for each individual cell are added to RNA or DNA prior to library preparation
3. Sequencing
Barcoded libraries are pooled and sequenced on the Illumina platform for high-throughput characterization
4. Data Analysis
Visualize results through interactive software and receive custom analysis
Features & Benefits
Superior Data Quality
Exceeding manufacturer’s benchmarks
Real-Time Project Updates
Through our online system
Ph.D.-Level Support
At every step
Early adopter of 10x Genomics Chromium with optimized workflows that maximize project flexibility, speed, and data accuracy
Highest throughput sequencing platforms, including the Illumina NovaSeq, provide cost-effective single-cell sequencing solutions
Samples accepted at different stages of the 10x workflow including cells, Gel Beads in Emulsion (GEMs), or 10x barcoded cDNA libraries for maximum workflow flexibility
Proprietary cell freezing protocol maintains cell viability during transit and provides a convenient method to ship samples
Dead cell removal to improve data quality, especially for projects with suboptimal samples
Interactive analysis report provides an intuitive way to explore data and find biological insights (see example report here - login required)
Poster: Highly Multiplexed Single-Cell Transcriptome and Epigenome Profiling of Cryopreserved Tissue Enables Multi-Omic Tissue Characterization in Clinical Settings
Single-cell analysis typically relies upon immediate processing of fresh tissue, presenting limitations for translational and clinical applications. Learn how GENEWIZ’s robust cryopreservation workflow for downstream processing enabled high-quality, single-cell transcriptomic and epigenomic analysis of translational and clinical samples by coupling scATAC-seq and scRNA-seq.
Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal
Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.
Webinar: Advancements to the 10x Genomics Chromium™, Single-Cell RNA-Seq System
In this webinar, we review how recent advancements of the 10x Genomics Chromium™ system, coupled with GENEWIZ developmental efforts, can benefit the researcher. In addition, we spotlight the new Single-Cell V(D)J Solution, which can be used in conjunction with single-cell transcriptome profiling to identify pairing of full-length BCR and TCR sequences
NGS Platforms
GENEWIZ is a certified service provider for Illumina and Life Technology NGS platforms. For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.