Cell populations are seldom homogeneous and similar in their characteristics. While traditional bulk sequencing methods can be powerful, cost-effective tools for analysis, they cannot detect differences that arise between individual cells in complex environments. With recent developments in microfluidics and molecular barcoding, however, NGS-based technologies for genomic, transcriptomic, and epigenomic research have increasingly focused on the analysis of single cells, enabling scientists to examine the diversity of heterogenous cell populations and uncover new biological discoveries.
Azenta Life Sciences offers a suite of end-to-end single-cell sequencing solutions utilizing the latest technology on the market: the 10x Genomics®Chromium™ and Illumina® NovaSeq™. As an early adopter of these platforms, our optimized workflows — including pre-submission cryopreservation and post-submission dead cell removal — maximize project flexibility, speed, and data accuracy.
For single-cell and single-nuclei RNA-Seq projects, utilize our interactive data portal powered by ROSALIND to receive robust analysis and publication-ready visuals of your sequencing data.
LIVE WORKSHOP & ROUNDTABLE DISCUSSION
Exploring Bioinformatics for
Single-Cell Sequencing Data
Wednesday, October 5, 2022 at 11AM (ET)
Single-Cell RNA-Seq
Analyze transcriptome heterogeneity at the single-cell level. GENEWIZ offers optimized workflows for 5’ and 3’ gene expression libraries to uncover cellular differences that are masked by bulk RNA sequencing and visualize sequencing data via our interactive data portal, powered by ROSALIND.
Single-Cell ATAC-Seq
Identify chromatin accessibility in the epigenome by profiling thousands of single cells in parallel, and discover how chromatin structure and DNA-binding proteins regulate gene expression in varying states and cellular processes.
Single-Cell Immuno-Profiling
Examine the immune repertoires of individual B and T cells by physically pairing VH/VL or α/β chains in B- or T-cell receptors, respectively, while simultaneously capturing data for gene expression, V(D)J clone types, and cell surface protein expression.
Cells and barcoded beads are isolated in droplets using the 10x Genomics Chromium
2. Library Preparation
Unique barcodes for each individual cell are added to RNA or DNA prior to library preparation
3. Sequencing
Barcoded libraries are pooled and sequenced on the Illumina platform for high-throughput characterization
4. Data Analysis
Receive custom analysis and visualize results through interactive software, including our data portal powered by ROSALIND for sc / snRNA-Seq projects
Features & Benefits
Superior Data Quality
Exceeding manufacturer’s benchmarks
Real-Time Project Updates
Through our online system
Ph.D.-Level Support
At every step
Early adopter of 10x Genomics Chromium with optimized workflows that maximize project flexibility, speed, and data accuracy
Highest throughput sequencing platforms, including the Illumina NovaSeq, provide cost-effective single-cell sequencing solutions
Samples accepted at different stages of the 10x workflow including cells, Gel Beads in Emulsion (GEMs), or 10x barcoded cDNA libraries for maximum workflow flexibility
Proprietary cell freezing protocol maintains cell viability during transit and provides a convenient method to ship samples
Dead cell removal to improve data quality, especially for projects with suboptimal samples
Visualize resultsthrough interactive software, including our data portal powered by ROSALIND for sc / snRNA-Seq projects
Technical Resources
Webinar Series | Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond
With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings.
Webinar: Improving Single-Cell RNA-Seq Workflow Efficiency to Scale Clinical Research
In this webinar co-hosted by Azenta Life Sciences and 10x Genomics, learn how Clinical Research Organizations (CROs) provide expertise across the single-cell RNA sequencing (scRNA-Seq) workflow, including sample prep and experimental execution. This enables pharmaceutical and biotech clients to perform single-cell analysis at greater scale, efficiency, and reproducibility for clinical research.
Poster: Highly Multiplexed Single-Cell Transcriptome and Epigenome Profiling of Cryopreserved Tissue Enables Multi-Omic Tissue Characterization in Clinical Settings
Single-cell analysis typically relies upon immediate processing of fresh tissue, presenting limitations for translational and clinical applications. Learn how Azenta’s robust cryopreservation workflow for downstream processing enabled high-quality, single-cell transcriptomic and epigenomic analysis of translational and clinical samples by coupling scATAC-seq and scRNA-seq.
Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal
Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how Azenta scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.
Webinar: Advancements to the 10x Genomics Chromium™, Single-Cell RNA-Seq System
In this webinar, we review how recent advancements of the 10x Genomics Chromium™ system, coupled with Azenta developmental efforts, can benefit the researcher. In addition, we spotlight the new Single-Cell V(D)J Solution, which can be used in conjunction with single-cell transcriptome profiling to identify pairing of full-length BCR and TCR sequences
Top 3 Factors to Consider Before Starting a Single-Cell Sequencing Project
With several commercial platforms available, high-throughput single-cell sequencing is now more accessible than ever. In this article, we’ll touch upon the 3 most important factors to consider before embarking on your single-cell project to ensure you select the right NGS approach for your research.
Webinar: Towards High Dimensional Sequencing: From Single-Cell to Spatial Omics
In comparison to traditional profiling methods which assess bulk populations, single-cell technologies empower researchers to examine diversity of heterogeneous cell populations and uncover new, and potentially unexpected, biological discoveries. This webinar highlights the unparalleled capabilities of single-cell sequencing.
Case Study: Single-Cell RNA-Seq Analysis Identifies Rare Drug-Resistant Cancer Stem Cells
Cell populations are rarely homogeneous and synchronized in their characteristics. Standard RNA-Seq approaches are limited to reporting general expression levels thus omitting minor subpopulation profiles. This study highlights new single-cell RNA sequencing capabilities for identifying rare cells, characterizing their transcriptomes, and discovering potential biomarkers.
Poster | Utilizing an alternate cell fixation method to expand opportunities for single-cell RNA sequencing
The complexity of sample preparation for single-cell sequencing remains a bottleneck for researchers interested in performing analysis at the cellular level. In this poster, we highlight an alternative sample preparation and processing workflow to standard methods that help to broaden the usage of single-cell sequencing approaches.
NGS Platforms
Azenta is a certified service provider for Illumina and Life Technology NGS platforms. For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.
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