Oligo Synthesis FAQs

A:
Oligo-Rapid : Receive standard, desalted oligos (15-60 nt at 25 or 50 nmol) in 1-2 business days. Expedited orders requested by 10 AM ET are shipped out the same night.

Customers from the Boston region can take advantage of our standard next-day service. Orders placed by 6 PM ET will be delivered by courier the following afternoon. Next-day service requires installation of a GENEWIZ incoming sample pick-up box. Please contact dnaseq@genewiz.com for any questions or to set up a box.

Oligo-Flex : Synthesis of custom length oligos (5-150 nt) at 25 - 1000 nmol scales. 5', internal, or 3' modifications are available with HPLC, PAGE, and desalted purification options. Turnaround time starts at 4 business days, depending on scale and modification requirements.

Oligos Retained for Sanger : Order an oligo for a Sanger sequencing order. GENEWIZ will synthesize, dilute, and store the oligo on-site for up to 1 year.
A:
Oligos are chemically synthesized using the industry-standard, solid-phase phosphoramidite method. Oligos are synthesized through a series of coupling reactions in the 3' and 5' direction.
A:
The GENEWIZ Oligo-Rapid service accepts standard, desalted primers between 15-60 nt.

The GENEWIZ Oligo-Flex service can synthesize oligos between 5-150 nt.
A:
Yes, GENEWIZ offers synthesis of oligos containing degenerate bases using the standard IUPAC base nomenclature.

IUPAC Code Base
R A or G
Y C or T
M A or C
K G or T
S C or G
W A or T
H A, C, or T
B C, G, or T
V A, C, or G
D A, G, or T
N A, C, G, or T
A:
Oligo-Rapid service ships orders within 1-2 business days.

Oligo-Flex service ships orders in as few as 4 business days depending on the modifications or purifications requested. Please refer to your order for specific turnaround time information.

Oligos Retained for Sanger will be available for sequencing within 1-2 business days depending on the time the order is placed.
A:
Oligos can be delivered dry, wet in water, or in certain buffers (upon request).
A:
If primers arrive dry, we recommend briefly centrifuging the tube to ensure that the DNA is at the bottom of the tube. Resuspend the sample in a volume or concentration appropriate for your needs and vortex briefly to mix well. Let the sample sit for 1-2 minutes. Before use, vortex briefly again.

*Please note the reconstitution solution is dependent on downstream applications and that TE may interfere with various enzymatic reactions.
A:
Dry oligos are stable and can be stored at -20°C for 1 year. Resuspended primers at higher concentrations (≥100 µM, in TE or water) are stable for 6 months at -20ºC.

*Please note that repeated freezing and thawing will reduce stability and shelf life.
A:
Purification Lengths (nt) Purity Recommended Uses
Desalted 5-90 >70% Standard sequencing and PCR primers
PAGE 11-150 >90% Long oligos
HPLC 5-90 >95% Modified oligos or oligos used in sensitive applications
A:
GENEWIZ synthesizes primers on a per-scale basis, which refers to the initial amount of material used in synthesis. Due to the inefficiency of the coupling reactions, yields are never equal to the initial amount of starting material. Yield is typically impacted by the nature and content of the sequence. Purification and modifications will also reduce the final yield delivered.

Length (nt) Scale Purification Minimum Guaranteed Yield
5-90 25 nmol Desalted 5 nmol
HPLC 2 nmol
50 nmol Desalted 10 nmol
HPLC 4 nmol
PAGE 2 nmol
100 nmol Desalted 20 nmol
HPLC 8 nmol
PAGE 4 nmol
200 nmol Desalted 50 nmol
HPLC 20 nmol
PAGE 10 nmol
1 µmol Desalted 300 nmol
HPLC 120 nmol
PAGE 60 nmol
91-150 100 nmol PAGE 2 nmol
200 nmol PAGE 5 nmol
1 µmol PAGE 25 nmol
A:
Dye Max Ex (nm) Max Em (nm) Color Compatible Quenchers
6-FAM 495 520 Green BHQ-1, BHQ-2, ECLIPSE, TAMRA, Dabcyl
JOE 520 548 Orange BHQ-1, ECLIPSE, Dabcyl, TAMRA
TET™ 521 536 Orange BHQ-1, BHQ-2, ECLIPSE, TAMRA, Dabcyl
HEX™ 520 548 Pink BHQ-1, BHQ-2, ECLIPSE, TAMRA, Dabcyl
TAMRA 544 576 Rose BHQ-2, ECLIPSE, Dabcyl
ROX™ 576 601 Red BHQ-2, ECLIPSE
Cy®5 650 670 Violet BHQ-2 
Cy®3 550 570 Red BHQ-2, ECLIPSE
TexasRed® 583 603 Red BHQ-2, ECLIPSE
A:
GENEWIZ offers several options for synthesis and cost depends on the synthesis type, length, scales, modifications, and purifications required. Please contact dnaseq@genewiz.com or +1-877-GENEWIZ (436-3949) ext. 2 with pricing inquiries or reach out directly to your Sales Representative.

Have a specific question?

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)