PCR + Sanger (R&D, GLP)

Sample Submission Guidelines

Nucleic Acid Extraction

Sample Type R&D GLP
Frozen mammalian cell pellet samples At least 1 X 106 to 1 x 107 cells per pellet* At least 5 x 106 cells per pellet (2 pellets preferred), recommended 1 x 107 cells per pellet*
Frozen mammalian tissue At least 25 mg* At least 25 mg*
FFPE Tissue (slides or curls) 1-2 slices per sample, 5-10 µm per slice/curl Not available
Blood samples 200 µl blood 200 µl blood
Buccal swabs At least 1 per sample Not available
Saliva Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount) Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)

SNP/Mutation Analysis

Sample Type R&D GLP
Genomic DNA samples ≥60 ng per amplicon per sample, conc 20 ng/µl, in water, elution buffer, or low TE buffer* At least 200 ng per sample, conc 20 ng/µl*
Frozen mammalian cell pellet samples At least 1 x 106 to 1 x 107 cells per pellet* At least 5 x 106 cells per pellet (2 pellets preferred), recommended 1 x 107 cells per pellet*
Frozen mammalian tissue At least 25 mg* At least 25 mg*
FFPE Tissue (slides or curls) 1-2 slices per sample, 5-10 µm per slice/curl Work can be performed on already extracted material from this sample type
Blood samples 200 µl blood 200 µl blood
Yeast Colony Agar plate with single and well isolated colonies Agar plate with single and well isolated colonies
Buccal swabs At least 1 per sample Not available
Saliva Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount) Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)

cDNA Verification

Sample Type R&D GLP
RNA At least 5 µg* At least 5 µg*
cDNA At least 200 ng per sample, conc 20 ng/µl* At least 200 ng per sample, conc 20 ng/µl*
Frozen mammalian cell pellet samples At least 1 x 106 to 1 x 107 cells per pellet* At least 5 x 106 cells per pellet (2 pellets preferred), recommended 1 x 107 cells per pellet*
Frozen mammalian tissue At least 25 mg* At least 25 mg*
FFPE Tissue (slides or curls) 1-2 slices per sample, 5-10 µm per slice/curl Work can be performed on already extracted material from this sample type
Blood samples 200 µl blood 200 µl blood
Viral particles

If cell free viral particles: 1 x 107 to 1 x 1012/particles per ml, submit 1 ml, 2 vials preferred*

If frozen allantoic fluid: at least 1 ml*

If cell free viral particles: 1 x 107 to 1 x 1012/particles per ml, submit 1 ml, 2 vials preferred*

If frozen allantoic fluid: at least 1 ml*


gDNA Verification

Sample Type R&D GLP
Genomic DNA samples ≥60 ng per amplicon per sample, conc 20 ng/µl, in water, elution buffer, or low TE buffer* At least 120 ng per sample, conc 20 ng/µl*
Frozen mammalian cell pellet samples At least 1 x 106 to 1 x 107 cells per pellet* At least 5 x 106 cells per pellet (2 pellets preferred), recommended 1 x 107 cells per pellet*
Frozen mammalian tissue At least 25 mg* At least 25 mg*
FFPE Tissue (slides or curls) 1-2 slices per sample, 5-10 µm per slice/curl Work can be performed on already extracted material from this sample type
Blood samples 200 µl blood 200 µl blood
Virus

If cell free viral particles: 1 x 107 to 1 x 1012/particles per ml, submit 1 ml, 2 vials preferred*

If frozen allantoic fluid: at least 1 ml*

If cell free viral particles: 1 x 107 to 1 x 1012/particles per ml, submit 1 ml, 2 vials preferred*

If frozen allantoic fluid: at least 1 ml*

Buccal swabs At least 1 per sample Not available
Saliva Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount) Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)

Fragment Analysis

Sample Type R&D GLP
Labeled PCR products 10-20 µl equivalent of PCR product  Not available

16S rRNA and Fungal ITS Sequencing

Sample Type R&D GLP
Bacterial colony on agar Agar plate with single and well isolated colonies Not available
gDNA At least 60 ng per sample, conc 20 ng/µl, homogeneous samples only for this service Not available
Glycerol stocks 200 µl of frozen stock on dry ice, homogeneous samples only for this service Not available
Agar stabs Agar stab with clonal sample

Not available

Cell Pellets 10cells, dry or resuspended in buffer

Not available

Fungal colony on agar Agar plate with single and well isolated colonies

Not available


Plasmid Sequencing

Sample Type R&D GLP
Plasmid DNA Primer Walking At least 5 µg*
If transforming- At least 500 ng
Glycerol stock Primer Walking >200 µl of frozen stock on dry ice, 2 stocks preferred*
Bacterial colony on agar Primer Walking Agar plate with single and well isolated colonies

Yeast Colony Sequencing

Sample Type R&D GLP
Yeast Colonies Agar plate with single and well isolated colonies Agar plate with single and well isolated colonies

Large-Scale DNA Preparation

Sample Type R&D GLP
Plasmid DNA Preps At least 5 µg
If transforming - At least 500 ng
Glycerol stock Preps 200 µl of frozen stock on dry ice, 2 stocks preferred*
Bacterial colony on agar Preps Agar plate with single and well isolated colonies

*Requires Dry-Ice Shipment


SHIPMENT INSTRUCTIONS FOR ALL R&D-LEVEL SERVICES

Recommended Packaging and Shipping Guidelines:

Please secure all samples for shipment as appropriate:

  1. ≤ 36 samples: Please submit samples in 1.5 ml snap-lock microcentrifuge tubes or 8-strip tubes with securely fastened lids. Parafilm can be used to minimize risk to samples during shipment.
  2. >36 samples: Please submit samples arrayed on a 96-well plate, sealed with an appropriate adhesive plate seal and centrifuged to collect samples in the wells.
  3. Agar plates: Please ship agar plates in a sturdy container with the agar-side up to prevent condensation build-up. Secure agar plate lids with tape, rubber bands, or parafilm to minimize risk to samples during shipment.
  4. Glycerol stocks, RNA, and samples for extraction should be shipped using dry ice. Please consider the estimated delivery date of your shipment when determining the amount of dry ice to use.

Note: GENEWIZ accepts purified genomic DNA or can extract genomic DNA from various sample types, including biosafety level 1 (BSL1) and 2 (BSL2) supplied sources. We do not accept samples that require a higher level of processing than BSL 2.


Direct Shipping (FedEx, UPS or World Carrier):

If a Drop Box is not available, or if your starting material requires dry ice, please use an overnight delivery service such as FedEx, UPS, or DHL. A print-out of the Excel order page must be included with the material.


All direct shipments should be sent to:
GENEWIZ
Attn: Molecular Genetics
115 Corporate Blvd
South Plainfield, NJ 07080


GENEWIZ Drop Box (if available):
Starting material that is stable at room temperature may be submitted via a GENEWIZ Drop Box (when available). A print out of the Excel order page must be included with the material. Contact dnaseq@genewiz.com to request a Drop Box pick-up for your location or if you have any Drop Box questions.



SHIPMENT INSTRUCTIONS FOR ALL GLP-COMPLIANT SERVICES

All shipments via FedEx, UPS or World Courier should be sent to:

Dry ice, ship to:
Attn: GLP Services
111 Corporate Boulevard
South Plainfield, NJ 07080
USA

Please include a copy of the Sample Registration Form.