MolecUlar Genetics (R&D, GLP)

Sample Submission Guidelines

16s rRNA Sequencing

Sample Type R&D GLP
Bacterial colony on agar Agar plate with single and well isolated colonies Not available
gDNA At least 60 ng per sample, conc 20 ng/µl, homogeneous samples only for this service Not available
Glycerol stocks 200 µl of frozen stock on dry ice, homogeneous samples only for this service Not available
Agar stabs Agar stab with clonal sample Not available

Fragment Analysis

Sample Type R&D GLP
Labeled PCR products 10-20 µl equivalent of PCR product  Not available

Nucleic Acid Extraction

Sample Type R&D GLP
Frozen mammalian cell pellet samples At least 1 X 10to 1 x 107 cells per pellet At least 5 x 106  to 1 x 107 cells per pellet (2 pellets preferred), can exceed 1 x 107  cells per pellet
Frozen mammalian tissue At least 25 mg At least 25 mg
FFPE slides 4 slices per sample, at least 20 µm per slice Not available
Blood samples 200 µl of blood Not available

cDNA Verification

Sample Type R&D GLP
RNA At least 5 µg At least 5 µg
cDNA At least 200 ng per sample, conc 20 ng/µl At least 200 ng per sample, conc 20 ng/µl 
Frozen mammalian cell pellet samples At least 1 x 106 to 1 x 107 cells per pellet, can exceed 1 x 107  cells per pellet At least 5 x 106  to 1 x 107 cells per pellet (2 pellets preferred), can exceed 1 x 107  cells per pellet
Frozen mammalian tissue At least 25 mg At least 25 mg
FFPE slides 4 slices per sample, at least 20 µm per slice Work can be performed on already extracted material from this sample type
FFPE tissue block 4 slices per sample, 10-20 µm per slice Work can be performed on already extracted material from this sample type
Blood samples 200 µl blood Work can be performed on already extracted material from this sample type
Virus If RNA: At least 5µg (~200µl) of RNA per sample (2 vials per sample)

If cell free viral particles:  1 x 107 to 1 x 1012 particles per ml, submit 1 ml, 2 vials preferred

If frozen allantoic fluid: at least 1 ml
 If RNA: At least 5µg (~200µl) of RNA per sample (2 vials per sample)

If cell free viral particles:  1 x 107 to 1 x 1012 particles per ml, submit 1 ml, 2 vials preferred

If frozen allantoic fluid: at least 1 ml

gDNA Verification

Sample Type R&D GLP
Genomic DNA samples At least 60 ng per amplicon per  sample, conc 20 ng/µl  At least 120 ng per sample, conc 20 ng/µl
Frozen mammalian cell pellet samples At least 1 x 106 to 1 x 107 cells per pellet, can exceed 1 x 107  cells per pellet

At least 5 x 106  to 1 x 107 cells per pellet (2 pellets preferred), can exceed 1 x 107  cells per pellet

Frozen mammalian tissue At least 25 mg At least 25 mg
FFPE slides 4 slices per sample, at least 20 µm per slice Work can be performed on already extracted material from this sample type
FFPE tissue block 4 slices per sample, 10-20 µm per slice Work can be performed on already extracted material from this sample type
Blood samples 200 µl blood Work can be performed on already extracted material from this sample type
Virus If RNA: At least 5 µg (~200µl) of RNA per sample (2 vials per sample)

If cell free viral particles:  1 x 107 to 1 x 1012 particles per ml, submit 1 ml, 2 vials preferred

If frozen allantoic fluid: at least 1 ml

If RNA: At least 5 µg (~200 µl) of RNA per sample (2 vials per sample)

If cell free viral particles:  1 x 107 to 1 x 1012 particles per ml, submit 1 ml, 2 vials preferred

If frozen allantoic fluid: at least 1 ml

SNP/Mutation Analysis

Sample Type R&D GLP
Genomic DNA samples At least 60 ng per sample, per amplicon, concentration ≥ 20ng/μL At least 200 ng per sample, conc 20 ng/µl
Frozen mammalian cell pellet samples At least 1 X 106 to 1 x 107 cells per pellet At least 5 x 106  to 1 x 107 cells per pellet (2 pellets preferred), can exceed 1 x 107  cells per pellet
Frozen mammalian tissue At least 25 mg  At least 25 mg
 FFPE slides 4 slices per sample, 10-20 µm per slice Work can be performed on already extracted material from this sample type
 FFPE tissue block 4 slices per sample, 10-20 µm per slice Work can be performed on already extracted material from this sample type
Blood samples 200 µl of blood Work can be performed on already extracted material from this sample type
Yeast Colony Agar plate with yeast colonies Agar plate with single and well isolated colonies

Plasmid Sequencing

Sample Type R&D GLP
Plasmid DNA Primer Walking
At least 5 µg per kb
Glycerol stock Primer Walking >200 µl of frozen stock on dry ice, 2 stocks preferred
Bacterial colony on agar Primer Walking Agar plate with single and well isolated colonies

Plasmid Preparation

Sample Type R&D GLP
Plasmid DNA Preps At least 5 µg
Glycerol stock Preps 200 µl of frozen stock on dry ice, 2 stocks preferred
Bacterial colony on agar Preps Agar plate with single and well isolated colonies

Yeast Colony Sequencing

Sample Type R&D GLP
Yeast Colonies Agar plate with single and well isolated colonies Agar plate with single and well isolated colonies

Shipment Instructions for Fragment AnAlysis Service

GENEWIZ recommends DS-33: 6FAM, PET, NED, and VIC for assay development; we reserve the LIZ channel for size standard.

Prepare samples in either 8-strip tubes or a 96-well plate. For orders with less than 48 samples, please use 0.2 ml, 8-strip PCR tubes. For orders with more than 48 samples, discount pricing is available for 96-well PCR plates. Please use 8-strip caps for your tubes or wells and wrap with parafilm; this is the best way to prevent drying and cross-contaminating during shipment.

Sample shipment: Please use a local Drop Box or Courier at your institution; please contact us for a complete list of local Drop Boxes and Courier pick-up locations. If a Drop Box or Courier pick-up location is not available at your institution, please ship your samples at room temperature via FedEx Overnight Service to:

GENEWIZ-MD
Attn: Fragment Analysis
7210 Corporate Court
Suite C
Frederick, MD 21703
USA
908-222-0711


SHIPMENT INSTRUCTIONS FOR ALL R&D-LEVEL SERVICES EXCEPT FRAGMENT ANALYSIS

Direct Shipping (FedEx, UPS):
If a Drop Box is not available, or if your starting material requires dry ice, please use an overnight delivery service such as FedEx or UPS. A print out of the Excel order page must be included with the material.
 
All direct shipments should be sent to:  
 
GENEWIZ
Attn: Project Management
115 Corporate Boulevard
South Plainfield, NJ 07080
USA
908-222-0711

GENEWIZ Drop Box (if available):
Starting material that is stable at room temperature may be submitted via a GENEWIZ Drop Box (when available).  A print out of the Excel order page must be included with the material. Contact dnaseq@genewiz.com to request a Drop Box pick-up for your location or if you have any Drop Box questions.
 

Shipment Instructions for ALL GLP-Compliant Services except for Small Genome NGS

All direct shipments via FedEx, UPS or World Courier should be sent to:  

GENEWIZ
MG-GLP
126 Corporate Boulevard
South Plainfield, NJ 07080
USA
908-222-0711

Please include a copy of the Sample Registration Form.