Confirming genome edits with Sanger sequencing is an important step in determining the efficiency of CRISPR-mediated genome editing. Our CRISPR Sanger sequencing service is designed to verify the successful edit of your region of interest, starting with cells or genomic DNA.
Request a Quote
CRISPR MUTATION ANALYSIS
Our Mutation Analysis service includes PCR optimization, amplicon production and purification, and Sanger sequencing for your CRISPR regions of interest. Whether you are confirming that a mutation has occurred or precisely determining the genotype and specific allelic event, leveraging our expertise ensures that your genome editing functions efficiently.
For CRISPR experiments, our experienced scientists can interrogate your specified on-target regions of interest. GENEWIZ can also confirm off-target effects of the RNA-guided Cas9 nuclease1. Simply identify your target regions and let our Sanger experts handle the rest.
1 Nucleic Acids Research (2014) doi: 10.1093/nar/gku402. CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences. Lin, Y, et. Al.
GENEWIZ PCR PLUS SEQUENCING CRISPR ANALYSIS SERVICES
Extraction of genomic DNA from single-isolate cell samples.
Amplicon selection and primer design for on- and off-target regions.
Assay development and optimization.
PCR amplification of genomic DNA.
High-throughput PCR purification and bi-directional DNA sequencing.
Automated and manual sequencing results analysis.
Report identifying nucleotide-level mutations.
Translated amino acid calls available upon request.
FEATURES & BENEFITS
Reliable Assay Development: Our highly-trained scientists develop PCR assays targeted to your region of interest.
Flexible Starting Materials: GENEWIZ can start with purified genomic DNA templates or extract it from supplied sources.
High-Throughput Processing: Target regions are amplified using optimized assays; amplicons are purified and sequenced by capillary electrophoresis in our best-in-class facility.
Accurate Data Analysis: Mutations are identified with both automated and manual analysis to ensure accuracy.
GENEWIZ vs. In-house Turnaround Time Comparison of PCR Sequencing CRISPR Analysis
GENEWIZ TURNAROUND: As fast as 5 DAYS
IN-HOUSE TURNAROUND: 2-4 WEEKS
- Amplicon selection and primer design to target regions of interest
- Assay development and optimization
- PCR amplification of genomic DNA using optimized conditions
- High-throughput PCR purification and bi-directional DNA sequencing per amplicon
- Automated and manual sequencing results analysis