AAV Plasmid Preparation

 

Preserving the integrity of inverted terminal repeat (ITR) sequences in adeno-associated virus (AAV) plasmids is important for the production of recombinant AAV (rAAV) vectors. GENEWIZ’s proprietary AAV plasmid preparation service delivers superior quality for midi to giga-scale AAV vectors, while maintaining the integrity of ITR regions. Our new process includes pre- and post-preparation quality controlled (QC) steps that leverage GENEWIZ's AAV-ITR Sanger sequencing capabilities for full sequence verification and ensures delivery of intact ITR clones for your research.


Preserve ITR INTEGRITY in aav vectors

Truncated ITRs can reduce the yield of full viral capsid production and increase generation of undesirable non-rAAV encapsidated DNA. Unfortunately, AAV plasmid ITRs are frequently mutated during plasmid production in bacterial cells. GENEWIZ’s AAV plasmid preparation service incorporates a two-step QC process, including pre-prep and post-prep QC. The pre-prep QC step assesses your sample configuration for the presence of full-length intact or truncated ITRs, or a mixture of both. This allows selected propagation of intact ITR sub-populations only, while the post-prep QC step ensures integrity of ITR regions in the final plasmids delivered to you. For whole plasmid sequencing, utilize our primer walking service with AAV plasmid preparation.

 

*Plasmid DNA is currently the only starting material accepted

Note: Screening and selection of intact ITR clones incurs additional cost



Features & Benefits



Robust 2-step QC process with sequence verification using GENEWIZ’s proprietary AAV-ITR sequencing.

Turnaround time starting at 6 business days** with Ph.D.-level support throughout project.


High-quality midi- to giga-scale preps available.


High success rate compared to standard plasmid preparation kits and protocols.


Multiple analysis options available, including sterility testing, restriction digest, full plasmid sequencing, etc.


Screening process to isolate intact ITRs in mixed populations.

**6-day turnaround time for samples that have previously been ITR sequence verified at GENEWIZ.

 

 

 

AAV Plasmid Preparation Technical Information

 

GENEWIZ has recently developed a proprietary AAV plasmid preparation process, which significantly improves the chance of maintaining ITR integrity, even when other commercially available competent cells growing in low temperature have failed. This process can also isolate clones with full-length ITR from a sample mixture containing intact and truncated ITRs.

AAV-ITR Sequencing Tech Note

 

GENEWIZ has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids.

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that
direct-sequencing templates are not available for our Same Day service.


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3)