DNA Sample Submission Guidelines

Whole Genome Sequencing / Low-Pass WGS / Shotgun Sequencing

• Sample Type: Genomic DNA (RNA-free)
• Sample Purity (OD260/280): 1.8-2.0
• Recommended Quantity: >1.5 µg, >20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry Ice, overnight

Single-Cell ATAC-Seq

• Sample Type: Frozen cell suspension according to Azenta-supplied protocol, submitted in duplicate
• Sample Viability: >90% post-thaw
• Recommended Quantity: >1M cells in 1 mL
• Minimum Quantity: 50,000 cells in 500 µL
• Resuspension Buffer: Appropriate cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)
• Shipment Method: Dry ice, overnight

ChIP-Seq

• Sample Type: ChIP DNA (RNA-free)
• Sample Purity (OD 260/280): 1.8-2.0
• Recommended Quantity: >50 ng
• Minimum Amount: 10 ng
• DNA Fragment Length: 100-800 bp; 200-600 bp (Main peak)
• Resuspension Buffer: Water, BE, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice, overnight

Exome Enrichment Kit, OncoGxOne™ Discovery Cancer Panels and TargetGxOne™ Gene Enrichment

• Sample Type: Genomic DNA (RNA-free)
• Sample Purity (OD260/280): 1.8-2.0
• Recommended Quantity: >1.5 µg, >20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice, overnight

Custom TargetGxOne™ Amplicon Sequencing

• Sample Type: Genomic DNA
• Sample Purity (OD260/280): 1.8-2.0
• Recommended Quantity: >1 µg, >20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice, overnight

16S-EZ & ITS-EZ

• Sample Type: Genomic DNA
• Sample Purity (OD260/280): 1.8-2.0
• Concentration: 20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice, overnight

Pre-Made Sequencing Libraries

• Recommended Quantity: >15 nM
• Volume: ≥20 ul
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice, overnight

genoTYPER-NEXT

• Sample Type: Fresh/frozen cell pellet, washed in PBS
• Recommended Quantity: ≥10⁶ cells
• Shipment Method: Dry ice, overnight

• Sample Type: DNA
• Recommended Quantity: 3 ug
• Resuspension Buffer: Tris Buffer
• Shipment Method: Dry ice, overnight

Amplicon-EZ

• Sample Type: Purified PCR products
• Sample Purity (OD260/280): 1.8-2.0
• Concentration: 20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Room temperature/blue ice/dry ice, overnight

Plasmid-EZ

• Sample Type: Purified plasmid DNA
• Size: Up to 25 kb; if larger than 25 kb, please reach out to our NGS experts
• Minimum Amount: 500 ng (we recommend using a dsDNA quantification assay such as Qubit™ or PicoGreen™); at least 2 μg of plasmid DNA is required for downstream plasmid DNA preparation
• Concentration: Normalized to 50 ng/µL
• Purity (A260/280): 1.8-2.0
• Buffer: Water, EB, or low TE (<0.1 mM EDTA)

RNA Sample Submission Guidelines

We now offer RNA Stabilization Tubes. Ship RNA safely at ambient temperature. Download the information sheet. Contact us to receive a free trial.

Standard/Strand-Specific RNA-Seq & Immuno-Profiling

• Sample Type: Total RNA (DNA-free)
• Sample Purity (OD260/OD280): 1.8-2.2
• Recommended RIN: ≥6.0
• Recommended Quantity: >2 µg, >50 ng/µl
• Minimum Quantity: 250 ng
• Resuspension Buffer: Nuclease-free water
• Shipment Method: Dry ice, overnight

Small RNA-Seq

• Sample Type: Total RNA (containing small RNA)
• Sample Purity (OD260/OD280): 1.8-2.2, DNA-free
• Recommended RIN: ≥6.0
• Recommended Quantity: ≥2 µg, >50 ng/µl
• Resuspension Buffer: Nuclease-free water
• Shipment Method: Dry ice, overnight

• Sample Type: Purified small RNA
• Sample Purity (OD260/OD280): 1.8-2.2, DNA-free
• Recommended RIN: ≥6.0
• Recommended Quantity: >50 ng, >50 ng/µl
• Resuspension Buffer: Nuclease-free water
• Shipment Method: Dry ice, overnight

Ultra-Low Input RNA-Seq

Cell number and buffer clearly indicated in Azenta Sample Submission Form

• Sample Type: Cells in solution or small amounts of total RNA
• Recommended Quantity: >1,000 cells/sample in Trizol or 20 ng of total RNA
• Shipment Method: Dry ice, overnight

Single-Cell RNA-Seq & Single-Cell Immuno-Profiling

• Sample Type: Frozen cell suspension according to Azenta-supplied protocol, submitted in duplicate; methanol-fixed cells per 10x Genomics^® guidelines are also accepted
• Sample Viability: >90% post-thaw
• Recommended Quantity: >1M cells in 500 µL
• Minimum Quantity: 50,000 cells in 500 µL
• Resuspension Buffer: Appropriate cryopreservation media (e.g., cell culture media supplemented with 20% FBS and 10% DMSO)
• Shipment Method: Dry ice, overnight

High-Throughput Gene Expression (HT-GEx) Screening

• Recommended Quantity: Frozen cell lysates, 5,000 - 20,000 cells in 15µl of lysis buffer
• Recommended Lysis Buffer: QIASeq UPX Cell Lysis Buffer (#333076) or equivalent
• Shipment Method: Dry ice, overnight
• Sample Container: PCR-clean 96-well PCR-plate -80°C dry-ice resistant and foil sealed
• Sample Type:
• Cell lysate
• Purified RNA (please contact us for details)

PacBio Iso-Seq

• Sample Type: Total RNA
• Sample Purity (OD260/280): 1.8 - 2.2
• Recommended RIN: ≥9.0
• Recommended Quantity: ≥2µg
• Recommended Volume: 10 - 20µL
• Shipment Method: Dry ice, overnight

Protein Sample Submission Guidelines

Olink Proteomics^®

Sample Preparation:

• Recommended Quantity: 30µl - 100µl at 0.5 µg/µL – 1.0 µg/µL of total protein concentration
• Minimum Amount: 20µl
• Shipping Method:Dry ice
• Sample Container:PCR-clean tubes or 96-well PCR plate  -80°C dry-ice resistant and easily resealable
• Sample Type:EDTA-plasma, interstitial fluids, cell lysate, microdialysis fluids, microvesicles, citrate-plasma, dried blood spots, synovial fluids, final needle biopsies, urine, saliva, tissue lysates, serum, cebrospinal fluids, cell culture media, heparin-plasma, plaque extracts

Extraction Requirements

FFPE Samples

• Recommended Quantity: ≥4 FFPE curls recommended
• Minimum Quantity: 2 FFPE curls
• Thickness: 5µm-20 µm
• Area: >150 mm²
• Shipping Method: Room temperature/blue ice

Cell pellets

• Eukaryotic:
• Recommended Quantity: 10⁶ cells
• Minimum Quantity: 10⁴ cells
• Microorganism:
• Recommended Quantity: 10⁸ cells
• Minimum Quantity: 10⁶ cells
• Shipping Method: Dry ice, overnight

Fresh Frozen Tissue

• Recommended Quantity: 30 mg
• Minimum Quantity: 2 mg
• Shipping Method: Dry ice, overnight

PacBio Blood

• Recommended Quantity: 10 mL
• Minimum Quantity: 3 mL
• Sample Quality: Collect blood in tubes containing EDTA and store at -70°C
• Shipping Method: Dry ice, overnight

PacBio Mammalian Cells

• Recommended Quantity: 2x10⁷ cells
• Minimum Quantity: 1x10⁷ cells
• Shipping Method: Dry ice, overnight

PacBio Yeast Cells

• Recommended Quantity: 7x10⁹ cells
• Minimum Quantity: 3.5x10⁹ cells
• Shipping Method: Dry ice, overnight

PacBio Bacterial Cells

• Recommended Quantity: 2.2x10¹⁰ cells
• Minimum Quantity: 1x10¹⁰ cells
• Shipping Method: Dry ice, overnight

PacBio Fresh Frozen Tissue

• Recommended Quantity: 100 mg
• Minimum Quantity: 50 mg
• Shipping Method: Dry ice, overnight

PacBio Viral Particles

• Recommended Quantity: 1x10¹³ vg in <1mL
• Minimum Quantity: 1x10¹² vg in <1mL
• Shipping Method: Dry ice, overnight

Viral Particles

• Recommended Quantity: 1x10¹² vg in <1mL
• Minimum Quantity: 1x10¹¹ vg in <1mL
• Shipping Method: Dry ice, overnight

Whole Blood

• Recommended Quantity: 2 tubes/sample
• Recommended Volume (DNA Extraction): 500 µl/tube
• Recommended Volume (RNA Extraction): 2.5 mL (whole blood, PAXgene Blood RNA Tube)
• Shipping Method: Fresh: Blue ice, frozen: Dry ice, overnight

Plasma/Serum

• Recommended Quantity: 2 tubes/sample
• Recommended Volume: 10 mL/tube
• Minimum Volume: 5 mL/tube
• Shipping Method: Dry ice, overnight

Stool

• Fecal pellet samples (solid, no buffer)
• Recommended Quantity: 100 mg
• Minimum Quantity: 50 mg
• Shipping Method: Dry ice/blue ice, overnight

Saliva

• Recommended Quantity: 1 mL
• Minimum Quantity: 500 uL
• Shipping Method: Dry ice/blue ice, overnight

Soil

• Recommended Quantity: 100 mg
• Minimum Quantity: 50 mg
• Shipping Method: Room temperature/blue Ice, overnight

Water

• Recommended Volume: 100 mL
• Minimum Volume: 20 mL
• Shipping Method: Room temperature

Swab

• Recommended Quantity: 2 tubes/sample; 1 swab/tube
• Shipping Method: Room temperature

Fixed Needle Core Biopsy

• Recommended Quantity: 4 biopsies
• Minimum Quantity: 1 biopsy
• Recommended Diameter: 1-2 mm diameter; >5 mm in length
• Shipping Method: Room temperature/blue ice

Snap Frozen Needle Core Biopsy

• Recommended Quantity: 10 mg
• Minimum Quantity: 3 mg
• Shipping Method: Dry ice, overnight

Azenta Sample Quality Control Assessment

Azenta performs QC free of charge with results reported within 24 – 48 hours.

*For High Molecular Weight DNA, gel electrophoresis will also be performed.

For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.

Phone: (877) 436-3949 ext. 1

Email: NGS@azenta.com