Next Generation Sequencing

Sample submission guidelines

DNA Sample Submission Guidelines

DNA can be concentrated free of charge via SpeedVacTM. Additional charges will apply for concentration via column or other methods.

Large Whole Genome Sequencing (>1Gb)

  • Sample type: genomic DNA
  • Sample purity: OD260/280: 1.8-2.0
  • Ideal quantity: >5µg, >80ng/µl
  • Minimum quantity with QC: 100ng
  • Bare minimum with no QC: 10ng
  • Re-suspended in water or TE (<0.1mM EDTA)

Small Whole Genome Sequencing

  • Sample type: genomic DNA
  • Sample purity: OD260/280: 1.8-2.0
  • Ideal quantity: >1 µg, >80ng/µl
  • Minimum quantity with QC: 100ng
  • Bare minimum with no QC: 1ng
  • Re-suspended in water or TE (<0.1mM EDTA)

ChIP-Seq

  • Sample type: ChIP DNA
  • Sample purity: OD 260/280: 1.8-2.2
  • Ideal quantity: > 50 ng
  • Minimum quantity with QC: 20ng
  • Minimum quantity without QC: 5ng
  • DNA fragment length: 500 bp-100 bp, main peak 200-300 bp
  • DNA should be Re-suspended in water or TE (< 0.1 mM EDTA)

Agilent SureSelect Exome Enrichment Kit, OncoGxOne™ Discovery Cancer Panels and TargetGxOne™ Gene Enrichment

  • Sample type: genomic DNA
  • Sample purity: OD260/280: 1.8-2.0
  • Ideal quantity: >5 µg, >80ng/µl
  • Minimum quantity with QC: 250ng
  • Bare minimum with no QC: 100ng
  • Re-suspended in water or TE (<0.1mM EDTA)

Ion AmpliSeq™ Cancer Hotspot Panel V2

    (Recommended for gDNA extracted from FFPE samples)
  • Sample type: genomic DNA
  • Sample purity: OD260/280: 1.8-2.0
  • Ideal quantity: >1µg
  • Minimum quantity with QC: 100ng
  • Bare minimum with no QC: 10ng
  • Re-suspended in water or TE (<0.1mM EDTA)

Illumina TruSeq Amplicon Cancer Panel

  • Sample type: genomic DNA
  • Sample purity: OD260/280: 1.8-2.0
  • Ideal quantity: >1µg
  • Minimum quantity with QC: 500ng
  • Bare minimum with no QC: 250ng
  • Re-suspended in water or TE (<0.1mM EDTA)

Custom TargetGxOne™ Amplicon Sequencing

  • Sample type: genomic DNA
  • Sample purity: OD260/280: 1.8-2.0
  • Ideal quantity: >1µg
  • Minimum quantity with QC: 100ng
  • Bare minimum with no QC: 10ng
  • Re-suspended in water or TE (<0.1mM EDTA)

16S MetaVx™ Panels

  • Sample type: genomic DNA
  • Sample purity: OD260/280: 1.8-2.0
  • Ideal quantity: >1µg, >80ng/µl
  • Minimum quantity with QC: 100ng
  • Bare minimum with no QC: 25ng
  • Re-suspended in water or TE (<0.1mM EDTA)

FuzeSeq™ Antibody Sequencing

  • Sample type: Mixed phagemid vector DNA
  • Sample purity: OD260/280: 1.8-2.0
  • Ideal quantity: >1µg
  • Minimum quantity with QC: 100µg
  • Bare minimum with no QC: 10ng
  • Re-suspended in water or TE (<0.1mM EDTA)
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RNA Sample Submission Guidelines

Standard RNA-Seq

  • Sample type: Total RNA (preferred) or cDNA (>1µg)
  • Sample purity: OD260/OD280: 1.8-2.2
  • RIN≥7.0 (measured on the Agilent2100 BioAalyzer, or equivalent)
  • Ideal quantity: >3µg for total RNA, >80ng/µl
  • Minimum quantity with QC: 50ng with polyA selection or 150ng for rRNA depletion
  • Bare minimum with no QC: 10ng with polyA selection or 100ng for rRNA depletion
  • Re-suspended in nuclease-free water
  • Shipped on dry/blue ice ONLY

Stranded RNA-Seq

  • Sample type: Total RNA (preferred) or cDNA (>1µg)
  • Sample purity: OD260/OD280: 1.8-2.2
  • RIN≥7.0 (measured on the Agilent2100 BioAnalyzer, or equivalent)
  • Ideal quantity: >5µg for total RNA, >80ng/µl
  • Minimum quantity with QC: 150ng for both polyA selection and rRNA depletion
  • Bare minimum with no QC: 100ng for both polyA selection and rRNA depletion
  • Re-suspended in nuclease-free water
  • Shipped on dry/blue ice ONLY

Small RNA-Seq

    GENEWIZ accepts total RNA or purified small RNA for small RNA-Seq projects.
  • Total RNA: 1 µg of total RNA in 5 µl nuclease free water
  • Purified small RNAs (purified from 1-10 µg of total RNA,) dissolved in molecular grade water or 10 mM Tris-HCL, pH 8.5 (< 0.1 mM EDTA)

Targeted RNA-Seq

  • Sample type: Total RNA (preferred) or cDNA (>1µg)
  • Sample purity: OD260/OD280: 1.8-2.2
  • RIN≥7.0 (measured on the Agilent2100 BioAnalyzer, or equivalent)
  • Ideal quantity: >5µg for total RNA, >80ng/µl
  • Minimum quantity with QC: 1µg
  • Bare minimum with no QC: 500ng
  • Re-suspended in nuclease-free water

Single Cell RNA-Seq

  • Sample Type: Cells in solution or small amounts of total RNA
  • Cell number clearly indicated in Sample Submission Form
  • For cells in PBS, we can prepare libraries directly from cell solution; cells in Trizol need RNA extraction (>500, <10,000 cells need special RNA extraction)
  • Both total RNA and cells in solution shipped on dry ice
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Extraction Requirements

All extractions are performed to best of GENEWIZ’s ability and the Client is charged for work performed regardless of outcome. Additional charges for procurement of kits may apply for select extractions.

FFPE samples (DNA and RNA)

  • ≥4 FFPE slides recommended, ≥2 slides with repeats, 1 slide minimum with no repeats
  • 5um-20um thickness each
  • Submitted on room temperature/blue ice

Cell pellets

  • Up to 106 cells, 104 cells minimum
  • GENEWIZ NGS does not provide cell culturing
  • Submitted on dry ice

Fresh Frozen Tissue

  • 1mg minimum, 10mg ideal, 20mg maximum
  • Submitted on dry ice

Viral Particles

  • >1mL supernatant
  • Viral titer >5x109 cells/mL
  • Submitted on dry ice

Whole Blood (DNA)

  • 2 tubes/sample (one used as backup) recommended
  • 200µl/tube
  • Submitted on blue ice

Plasma/Serum

  • 2 tubes/sample (one used as backup) recommended
  • 3-10mL/tube
  • Submitted on dry ice

Stool

  • 50-100mg, 250 mg maximum
  • Submitted on dry/blue ice

Soil

  • 50-100mg, 250 mg maximum
  • Submitted at room temperature/blue ice

Water

  • 20mL-100 mL
  • Submitted at room temperature

Swab

  • 2 tubes/sample (one used as backup) recommended
  • 1 swab/tube
  • Submitted at room temperature

Fixed Needle Core Biopsy

  • Minimum 1 needle core biopsy, optimum 3-4 biopsies
  • Submitted at room temperature/blue ice

Snap Frozen Needle Core Biopsy

  • 1-3mg tissue minimum, 20mg maximum
  • Submitted on dry ice
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 GENEWIZ Sample Quality Control Assessment

GENEWIZ performs QC free of charge with results reported within 24 hours.

  DNA RNA Pre-Made Libraries
Agilent 2100 BioAnalyzer  

Qubit Assay


Gel Electrophoresis
   

For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.

Phone: +1 (908) 222-0711 ext. 1

Email: NGS@genewiz.com

 

Direct Shipping (FedEx, UPS): 

Please use an overnight delivery service such as FedEx or UPS for all NGS samples. A print out of the confirmed quote must be included with the material.


All direct shipments should be sent to:

  
GENEWIZ 
Attn: Next Generation Sequencing 
115 Corporate Boulevard 
South Plainfield, NJ 07080 
USA 
908-222-0711