Next Generation Sequencing Sample Submission Guidelines
View our Sample Submission Best Practices Checklist, opens in a new tab .
Direct Shipping, FedEx
Please use an overnight delivery service such as FedEx for all gwz samples. A printout of the confirmed quote must be included with the material.
All direct shipments should be sent to:
GENEWIZ from Azenta Life SciencesAttn: NGS
111 Corporate Boulevard
Suite H Loading Dock
South Plainfield, NJ 07080
USA
Tubes / Plates
Organize your samples in tubes or plates following the order indicated on the sample submission form.
Tubes: Prepare samples in clearly labeled and well-organized 1.5 mL flip-cap microcentrifuge tubes. Please avoid using Parafilm to seal the tubes.
Plates: For projects with 16 or more samples, prepare samples in clearly labeled, securely sealed 96-well full-skirted PCR v-bottom plates. Arrange the samples vertically by column, such as A1, B1, C1, etc.
Quality control, QC, is conducted using Qubit, not Nanodrop, and TapeStation. QC results will determine the most suitable approach based on sample quantity and quality.
DNA Sample Submission Guidelines
For precise concentration measurements, a dsDNA quantification assay such as Qubit or PicoGreen is required.
DNA can be concentrated free of charge via SpeedVacTM. Additional charges and turnaround time will apply for concentration via column or other methods.
Pre-Made Library Sequencing, Sequencing Only
| Sample Type | Quantity | Quality | Shipping |
|---|---|---|---|
|
Illumina-compatible Library Samples* |
Quantity: ≥10 nM |
<1% adapter dimers |
Dry ice |
*Include indices for de-multiplexing, library type, and custom requirements in the Sample Submission Form.
Whole Genome Sequencing (Human/Non-human) / Low-Pass WGS
| Sample Type | Quantity | Quality | Shipping |
|---|---|---|---|
Genomic DNA Double-Stranded |
Preferred Amount |
A260/A280 = 1.8-2.0 |
Dry ice |
*Include indices for de-multiplexing, library type, and custom requirements in the Sample Submission Form.
Whole Genome Sequencing, PCR-free
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
Genomic DNA Double-Stranded |
Preferred Amount: |
A260/A280 = 1.8–2.0 |
Dry ice |
*Large data amounts may require additional starting material.
PacBio Whole Genome Sequencing
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
High Molecular Weight DNA* |
Preferred Amount: |
A260/A280 = 1.8–2.2 |
Dry ice |
*See Best Practices: Preparation of High Molecular Weight DNA .
Whole Exome Sequencing (WES)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Genomic DNA Double-Stranded |
Preferred Amount: |
A260/A280 = 1.8-2.0 |
Dry ice |
Amplicon Sequencing
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
Genomic DNA Double-Stranded* |
Preferred Amount: |
A260/A280 = 1.8–2.0 |
Dry ice |
*Purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids accepted.
PacBio Amplicon Sequencing
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
Amplicon DNA* |
Preferred Amount:
Quantity: ≥2 µg |
A260/A280 = 1.8–2.2 |
Dry ice |
*Purified and unpurified amplicons, restriction digested material, and plasmids accepted. Additionally, we can prepare an amplicon from a wide range of starting materials, including genomic DNA, cell pellets, and tissue material. See the Extraction Requirements section for further details.
Methyl-Seq
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Genomic DNA Double-Stranded |
Preferred Amount: |
A260/A280 = 1.8-2.0 |
Dry ice |
ChIP-Seq
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
ChIP DNA |
Preferred Amount: |
A260/A280 = 1.8-2.0 |
Dry ice |
ATAC-Seq
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Cryopreserved Cells |
Preferred Amount: |
≥90% cellular viability after thawing |
Dry ice, overnight |
10X Genomics® Next GEM Epi ATAC
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Cryopreserved Cells* |
Preferred Amount: |
≥90% cellular viability after thawing |
Dry ice, overnight |
| Isolated Nuclei | Preferred Amount: |
N/A | Dry ice, overnight |
| Fresh, Frozen Tissue | 10-50 mg** | N/A | Dry ice, overnight |
*Follow GENEWIZ-supplied protocol, submitted in duplicate (one as backup). For single sample submission, we will proceed with our best effort and services will be charged regardless of outcome.
*We offer optional nuclei isolation and tissue dissociation services for an additional fee.
**Quantity required dependent on tissue type.
OncoGxOne™ Discovery Cancer Panels / TargetGxOne™ Gene Enrichment
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Genomic DNA Double-stranded |
Preferred Amount: |
A260/A280 = 1.8-2.0 |
Dry ice |
Custom TargetGxOne™ Amplicon Sequencing
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Genomic DNA Double-stranded |
Preferred Amount: |
A260/A280 = 1.8-2.0 |
Dry ice |
genoTYPER-NEXT CRISPR Validation
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Control DNA |
Preferred Amount: |
A260/A280 = 1.8-2.0 | Dry ice, overnight |
Fresh/Frozen Cell Pellet |
Preferred Amount: |
Submit CRISPR-edited cell lines in 96-well plates |
Dry ice, overnight |
16S-EZ / ITS-EZ
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Genomic DNA Double-stranded |
Minimum Amount: |
A260/A280 = 1.8-2.0 |
Dry ice, overnight |
Amplicon-EZ (AMP-EZ)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Purified PCR Products |
Minimum Amount: |
A260/A280 = 1.8-2.0 | Ambient or blue ice |
Plasmid-EZ (Whole Plasmid Sequencing)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Plasmid DNA* |
Minimum Amount: |
A260/A280 = 1.8-2.0 | Ambient or blue ice |
Bacteria
|
|
|
|
*GENEWIZ will perform linearization upon receipt of plasmid DNA samples followed by library preparation and sequencing.
PCR-EZ (Long-Read PCR Product Sequencing)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Unpurified and Purified PCR Products |
Minimum Amount: |
A260/A280 = 1.8-2.0 | Ambient or blue ice |
PCR-EZ Max (Full-Length, Long-Read PCR Product Sequencing)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Unpurified and Purified PCR Products |
Minimum Amount: |
A260/A280 = 1.8-2.0 |
Ambient or blue ice |
Precision Antibody Discovery (For phage display libraries)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Amplicon DNA* |
Preferred Amount: |
A260/A280 = 1.8-2.0 |
Dry ice, overnight |
*For in vivo samples, we can accept total RNA or perform RNA extraction from frozen cell pellets. For further details, please see the Precision Antibody Discovery (For in vivo samples) section or the Extraction Requirements section.
RNA Sample Submission Guidelines
We offer RNA Stabilization Tubes to ship RNA safely at ambient temperature. Download the information sheet or contact us to receive a free trial.
Standard & Strand-Specific RNA-Seq
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Total RNA |
Preferred Amount: |
A260/A280 = 1.8-2.2 |
Dry ice |
Ultra-Low RNA-Seq
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Total RNA |
Preferred Amount: |
A260/A280 = 1.8-2.2 |
Dry ice |
Cells |
Minimum Amount: |
Clearly indicate cell number and buffer | Dry ice |
*No QC is performed to preserve limited quantity. Customer-provided QC results will be used for maximum 10ng input for library prep.
Small RNA-Seq
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Total RNA (containing small RNA) |
Preferred Amount: |
A260/A280 = 1.8-2.2 |
Dry ice |
PacBio Kinnex Full-Length RNA
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Total RNA |
Preferred Amount: |
A260/A280 = 1.8-2.2 |
Dry ice |
10X Genomics® GEM-X Universal (3’ / 5’) Gene Expression & Immuno-Profiling
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Cryopreserved Cell Suspension* |
Preferred Amount: |
≥90% cellular viability after thawing |
Dry ice, overnight |
Isolated Nuclei |
Preferred Amount: |
N/A |
Dry ice, overnight |
Fresh, Frozen Tissue |
10-50 mg** |
N/A |
Dry ice, overnight |
*Follow GENEWIZ-supplied protocol, submitted in duplicate (one as backup). For single sample submission, we will proceed with our best effort and services will be charged regardless of outcome.
*We offer optional nuclei isolation and tissue dissociation services for an additional fee.
**Quantity required dependent on tissue type.
10X Genomics® GEM-X Flex Gene Expression
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Cells, Nuclei, Tissue* |
Preferred Amount: |
N/A |
Dry ice, overnight |
FFPE Tissue Sections |
Preferred Amount: |
25 µm sections (2 x 25 µm for human, 2 x 50 µm for murine) |
Ambient |
*Follow GENEWIZ-supplied protocol, submitted in duplicate (one as backup). For single sample submission, we will proceed with our best effort and services will be charged regardless of outcome.
Spatial Transcriptomics
High-Throughput Gene Expression (HT-Gex) Screening
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Frozen Cell Lysates* |
Preferred Amount: |
N/A |
Dry ice |
*For purified RNA, contact us for details.
Immuno-Profiling
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Total RNA |
Preferred Amount: |
A260/A280 = 1.8-2.2 |
Dry ice |
Precision Antibody Discovery (For in vivo samples)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
Total RNA* |
Preferred Amount: |
A260/A280 = 1.8-2.0 |
Dry ice, overnight |
*We also accept frozen cell pellets for RNA extraction. Please see the Extraction Requirements section for further details or contact us to inquire about other types of starting material. For phage display libraries, we can accept amplicon DNA. Please see the DNA section for further details.
Protein Sample Submission Guidelines
Olink Proteomics®
| Sample Type | Volume | Quality | Shipping Temperature |
|---|---|---|---|
Cell-free Samples |
Preferred Amount: |
Cell lysates require 0.5 µg/µL – 1.0 µg/µL of total protein concentration |
Dry ice, overnight |
*We also accept frozen cell pellets for RNA extraction. Please see the Extraction Requirements section for further details or contact us to inquire about other types of starting material. For phage display libraries, we can accept amplicon DNA. Please see the DNA section for further details.
Extraction Requirements
All extractions are performed to the best of GENEWIZ’s ability, and the Client is charged for work performed regardless of outcome. Additional charges for procurement of kits may apply for select extractions. Please note: we do not accept samples above BSL 2 and we require prior notification for pathogenic samples.
The minimum submission ensures quality control and a single library preparation. Additional material may be necessary for any repeat analyses. Quality control (QC) is conducted using Qubit (not Nanodrop) and TapeStation. QC results will determine the most suitable approach based on sample quantity and quality.
FFPE Samples
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
FFPE Curls |
Preferred Amount: |
Thickness: 5µm-20 µm |
Ambient or blue ice |
Tissue
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
Tissue |
Preferred Amount: |
N/A |
Dry ice, overnight |
Cell Pellets
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
Eukaryotic |
Preferred Amount: |
N/A |
Dry ice, overnigh |
Microorganism |
Preferred Amount: |
N/A |
Dry ice, overnight |
Whole Blood
| Sample Type | Volume | Details | Shipping Temperature |
|---|---|---|---|
For DNA Extraction* |
Preferred Amount: |
N/A |
Fresh: Blue ice |
For RNA Extraction* |
Preferred Amount: |
PAXgene Blood RNA Tube |
Fresh: Blue ice |
*Submitted in duplicate (one as backup). For single sample submission, GENEWIZ will proceed with our best effort and services will be charged regardless of outcome.
Plasma / Serum
| Sample Type | Volume | Details | Shipping Temperature |
|---|---|---|---|
Plasma / Serum* |
Preferred Amount: |
N/A |
Dry ice, overnight |
*Submitted in duplicate (one as backup). For single sample submission, GENEWIZ will proceed with our best effort and services will be charged regardless of outcome.
Stool
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
Fecal Pellet |
Preferred Amount: |
Solid, no buffer |
Blue ice or dry ice |
Saliva
| Sample Type | Volume | Details | Shipping Temperature |
|---|---|---|---|
Saliva |
Preferred Amount: |
N/A |
Blue ice or dry ice |
Soil
| Sample Type | Volume | Details | Shipping Temperature |
|---|---|---|---|
Soil |
Preferred Amount: |
N/A |
Ambient or blue ice |
Water
| Sample Type | Volume | Details | Shipping Temperature |
|---|---|---|---|
Water |
Preferred Amount: |
N/A |
Ambient |
Swab
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
Swab* |
Minimum Amount: |
N/A |
Ambient |
*Submitted in duplicate (one as backup). For single sample submission, GENEWIZ will proceed with our best effort and services will be charged regardless of outcome.
Fixed Needle Core Biopsy
| Sample Type | Volume | Details | Shipping Temperature |
|---|---|---|---|
Fixed Needle Core Biopsy |
Preferred Amount: |
1-2 mm diameter; 5 mm length |
Ambient or blue ice |
Snap Frozen Needle Core Biopsy
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
Snap Frozen Needle Core Biopsy |
Preferred Amount: |
N/A |
Dry ice, overnight |
Viral Particles
| Sample Type | Concentration | Details | Shipping Temperature |
|---|---|---|---|
Viral Particles |
Preferred Amount: |
N/A |
Dry ice, overnight |
PacBio Fresh Frozen Tissue
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
PacBio Fresh Frozen Tissue |
Preferred Amount: |
N/A |
Dry ice, overnight |
PacBio Whole Blood
| Sample Type | Volume | Details | Shipping Temperature |
|---|---|---|---|
PacBio Whole Blood |
Preferred Amount: |
EDTA tubes and store at -70°C |
Dry ice, overnight |
PacBio Mammalian Cells
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
PacBio Mammalian Cells |
Preferred Amount: |
N/A |
Dry ice, overnight |
PacBio Yeast Cells
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
PacBio Yeast Cells |
Preferred Amount: |
N/A |
Dry ice, overnight |
PacBio Bacterial Cells
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
PacBio Bacterial Cells |
Preferred Amount: |
N/A |
Dry ice, overnight |
PacBio Viral Particles
| Sample Type | Quantity | Details | Shipping Temperature |
|---|---|---|---|
PacBio Viral Particles |
Preferred Amount: |
N/A |
Dry ice, overnight |
GENEWIZ Sample Quality Control Assessment
GENEWIZ performs QC free of charge with results reported within 24 – 48 hours.
| Quality Control | DNA* | RNA | Pre-Made Libraries |
|---|---|---|---|
| TapeStation | |||
| Qubit Assay |
*For High Molecular Weight DNA, gel electrophoresis will also be performed.
For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.
Phone: (877) 436-3949 ext. 1
Email: NGS@azenta.com