Custom mRNA Synthesis

 
With the development of in vitro transcription (IVT) methods, messenger ribonucleotides (mRNA) synthesis has become an emerging class of gene therapy. Serving as the molecular bridge between DNA and proteins, mRNAs and their in vitro transcription technology enable the concept of nucleic acid-encoded proteins for research and therapeutic purposes. The transient nature of mRNA activity also offers an alternative approach to gene editing and gene therapy for delivering genetic information into cells.

To support mRNA research and therapeutic applications, Azenta offers gene synthesis and custom mRNA synthesis with industry-leading project completion time, a wide range of deliverables, and best-in-class quality.

Applications of Synthetic mRNA

Therapeutic Development: Treat diseases of protein expression and cellular function without gene editing
Cancer Research: Express tumor antigens or chimeric antigen receptors (CARs) to investigate cancer treatment and response.
CRISPR/Gene Editing: Create targeting mRNA sequences for delivery of CRISPR/Cas9 and other gene editing platforms.
Antibody Development: Translate antibodies in vivo from encoded mRNA instead of from monoclonal production.

HOW DO YOU SYNTHESIZE MRNA?

Synthetic mRNA comes from a template nucleotide sequence of DNA. The process starts with codon optimization of this sequence for optimal transcription. The gene is synthesized, cloned into a vector, and verified by Sanger sequencing. The plasmid DNA is then amplified, target mRNA sequence linearized, and used as a template for in vitro transcription.

MRNA TranScription Service

 
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1. Construct Design


Codon Optimization:

Maximize protein expression and experimental performance with Azenta's propriety codon optimization tool

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2. DNA Synthesis

 

Oligo and Gene Synthesis: 99.9% reliability in project delivery rate from a team with over 20 years of gene synthesis experience

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3. Cloning and Plasmid Prep


Template Plasmid: 
Performed by our experienced molecular biologists to quickly generate plasmid DNA for IVT based on desired mRNA yield

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4. In Vitro Transcription


Scalable Transcription:
   
Mini- to giga-scale quantities of mRNA available to grow with your research program

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5. mRNA Purification and QC


High-Quality Ribonucleotides: 
RNA product generated from 100% Sanger sequence-verified template with size visualization and certificate of analysis (CoA)

Featured Application: mRNA Therapeutic Development

With the critical role of mRNA vaccines in global pandemic response to COVID-19, mRNA synthesis technology has strengthened its status as an important technique in therapeutic development. Sequence accuracy of the template plasmid and the quality of IVT mRNAs are two crucial factors in determining the success of downstream lead generation and characterization. A foundational expertise in gene synthesis accommodates a range of mRNA complexities (5’ cap and poly A tail) to achieve optimal protein expression and generate 100% sequence-verified plasmid constructs. Download the tech note to learn how Azenta achieves high-quality analytical and empirically performing mRNA products.

Azenta Features and Benefits

Rapid Project Completion – Gene synthesis to mRNA IVT in as fast as 2 weeks to meet your project deadlines
High Quality mRNA - Stringent quality control of IVT template with guaranteed 100% Sanger sequencing accuracy
Ph.D.–Level Customer Support - Our dedicated project management team provides reliable consultation and support for your project from start to finish
Effortless Project Management – Work with a single research partner for everything from construct design to delivery of purified mRNA
Flexible mRNA Delivery – Suit the needs of your project with a wide range of mRNA types, sequence lengths, and yield of final product

mRNA Synthesis Deliverables

mRNA products are provided as a dry powder, fully lyophilized and shipped at room temperature. Below is a summary of what to expect from your custom mRNA synthesis order. 


mRNA Synthetic Motifs Purified Synthetic Yield Synthetic Length
Estimated Completion Time Quality Control
ncRNA: Non-coding RNA

Cap0 mRNA: m7Gppp-capped mRNA with polyA tail

Cap1 mRNA: m7GpppNm-capped mRNA with polyA tail

Pseudouridine modification (optional)1






40 ug – 2000 ug
< 2000 bp In as few as
2 weeks
- 100% sequence accuracy from Sanger sequencing of template

- Certificate of analysis (CoA)

- Gel electrophoresis size visualization

- Agilent 2100 Bioanalyzer verification (optional)2
2001-4000 bp               In as few as
4 weeks
4001-6000 bp In as few as
5 weeks
 

> 6001 bp

 Custom
  1. Pseudouridine is a common RNA modification to replace uridine in IVT mRNA. It has been demonstrated to enhance RNA stability and decrease anti-RNA immune response.
  2. Agilent 2100 Bioanalyzer utilizes LabChip technology to automate the measurement of both the concentration and the integrity/purity of mRNA samples.

HOW TO ORDER


Email | Phone: 1-877-436-3949, Ext. 3) | Live Chat