RNA Sequencing (RNA-Seq) is a powerful method for studying the transcriptome qualitatively and quantitively. It can identify the full catalog of transcripts, precisely define the structure of genes, and accurately measure gene expression levels. GENEWIZ provides unparalleled flexibility in the analysis of different RNA species (coding, non-coding, and small transcripts) from a wide range of starting material using long- or short-read sequencing.

Standard RNA Sequencing is our most popular option for profiling gene expression, enabling you to analyze coding (mRNA) and long non-coding RNA (lncRNA). We also offer a multitude of RNA-Seq services to fit any of your research needs, which you can learn about below.



Additional RNA Sequencing Services

Single-Cell RNA-Seq

Single-Cell RNA Sequencing analyzes gene expression at the single-cell level for heterogeneous samples. The 10x Genomics Chromium™ platform provides industry-leading options for transcriptional profiling of thousands of individual cells.


Iso-Seq (isoform sequencing) reads full-length contiguous transcripts (up to 10 kb), elucidating the diversity of isoforms within the transcriptome. Applications include improved genome annotation, detection of gene fusions, novel transcript discovery, and alternative splicing analysis.

Ultra-Low Input RNA-Seq

Ultra-Low Input RNA Sequencing provides bulk expression analysis of samples containing as few as 10 pg of RNA or just a few cells. This service is ideal for analyzing cells after fluorescence-activated cell sorting (FACS). To learn more, read our case study.

Strand-Specific RNA-Seq

Strand-Specific RNA Sequencing provides gene expression information with relation to strand orientation. Recommended for analysis of anti-sense/overlapping transcripts, discovery of novel transcripts, and genome annotation.

Small RNA-Seq

Small RNA Sequencing enables analysis of miRNA and other small RNA species. Utilize this service to identify and measure a repertoire of small RNA molecules.

Features & Benefits

Superior data quality – Guarantee of ≥80% bases with ≥Q30 for 2x150 bp sequencing (>90% on average)

Industry-leading turnaround time – Express upgrade available

Dedicated support – Ph.D.-level study managers available at every step including post-delivery support

Complete sample-to-bioinformatics solution – Flexible extraction and data analysis options available

Technical Resources

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Case Study: Single-Cell RNA-Seq Analysis Identifies Rare Drug-Resistant Cancer Stem Cells

Cell populations are rarely homogeneous and synchronized in their characteristics. Standard RNA-Seq approaches are limited to reporting general expression levels thus omitting minor subpopulation profiles. This study highlights new single-cell RNA-sequencing capabilities for identifying rare cells, characterizing their transcriptomes, and discovering potential biomarkers.

Tech Note: Isoform Sequencing on the PacBio Sequel® – Maximizing Output and Accuracy

Contiguous mRNA full-length sequencing (Iso-Seq) greatly simplifies genome annotation efforts and revolutionizes the discovery of novel RNA isoforms. This tech note discusses the advantages of the latest technologies combined with GENEWIZ’s optimized workflow, and how this increases output and accuracy.

NGS Platforms

GENEWIZ is a certified service provider for Illumina and Life Technology NGS platforms. For information on our NGS platforms as well as recommended configurations for your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing only projects.

How To Order

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