PCR Purification


Azenta Life Sciences, formerly GENEWIZ offers the added convenience of our PCR Purification service to eliminate the need for costly and time-consuming column or gel-extraction clean ups. Our enzymatic PCR clean-up technique removes excess primers and unincorporated dNTPs from PCR sequencing templates prior to the cycle sequencing reaction. Let Azenta remove the hassle from PCR clean-up and expedite your PCR product sequencing workflow.

PCR Clean Up

Azenta uses a combination of enzymes to remove excess dNTPs and oligonucleotides from the PCR reaction prior to sequencing.  This approach generates a good-quality template that is ready to sequence, while avoiding common contamination problems from other, column-based purification techniques.

Enzymatic Purification Process

Enzymatic Purification Process

Technical Notes


In order to optimize the concentration of the sequencing reaction, GENEWIZ recommends submitting a representative gel image of your PCR products along with your samples and order receipt.

This approach purifies the entire PCR reaction, and can only be used for single-band PCR products. Occasionally, certain brands of high-fidelity Taq can cause interference with the enzymatic cleanup reaction. Please contact Technical Support if you would like to discuss your specific enzyme selection.

NEW Direct Colony to
Whole Plasmid Sequencing




Time savings and cost savings - no need for time-consuming column purifications

Analysis using ABI Prism 3730xl DNA analyzers
Free Azenta Universal Primers
Customized troubleshooting
Fast turnaround
>500 bases of Phred20 reads
Azenta Online Ordering and Data Retrieval System - 24/7 web-based
Project design and consultation

Troubleshooting DNA Templates
with Sanger Sequencing




*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.

Email | Phone (1-877-436-3949, Ext. 2)