PCR Purification

GENEWIZ offers the added convenience of our PCR Purification service to eliminate the need for costly and time-consuming column or gel-extraction clean ups. Our enzymatic PCR clean-up technique removes excess primers and unincorporated dNTPs from PCR sequencing templates prior to the cycle sequencing reaction. Let GENEWIZ remove the hassle from PCR clean-up and expedite your PCR product sequencing workflow.

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PCR Clean Up

GENEWIZ uses a combination of enzymes to remove excess dNTPs and oligonucleotides from the PCR reaction prior to sequencing.  This approach generates a good-quality template that is ready to sequence, while avoiding common contamination problems from other, column-based purification techniques.

Enzymatic Purification Process

Enzymatic Purification Process

Technical Notes


In order to optimize the concentration of the sequencing reaction, GENEWIZ recommends submitting a representative gel image of your PCR products along with your samples and order receipt.

This approach purifies the entire PCR reaction, and can only be used for single-band PCR products. Occasionally, certain brands of high-fidelity Taq can cause interference with the enzymatic cleanup reaction. Please contact Technical Support if you would like to discuss your specific enzyme selection.


Time savings and cost savings - no need for time-consuming column purifications

Analysis using ABI Prism 3730xl DNA analyzers
Free GENEWIZ Universal Primers
Customized troubleshooting
Fast turnaround
>500 bases of Phred20 reads
GENEWIZ Online Ordering and Data Retrieval System - 24/7 web-based
Project design and consultation


*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.

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