GENEWIZ offers the added convenience of our PCR Purification service to eliminate the need for costly and time-consuming column or gel-extraction clean ups. Our enzymatic PCR clean-up technique removes excess primers and unincorporated dNTPs from PCR sequencing templates prior to the cycle sequencing reaction. Let GENEWIZ remove the hassle from PCR clean-up and expedite your PCR product sequencing workflow.
GENEWIZ uses a combination of enzymes to remove excess dNTPs and oligonucleotides from the PCR reaction prior to sequencing. This approach generates a good-quality template that is ready to sequence, while avoiding common contamination problems from other, column-based purification techniques.
Enzymatic Purification Process
In order to optimize the concentration of the sequencing reaction, GENEWIZ recommends submitting a representative gel image of your PCR products along with your samples and order receipt.
This approach purifies the entire PCR reaction, and can only be used for single-band PCR products. Occasionally, certain brands of high-fidelity Taq can cause interference with the enzymatic cleanup reaction. Please contact Technical Support if you would like to discuss your specific enzyme selection.
FEATURES & BENEFITS
Time savings and cost savings - no need for time-consuming column purifications