Targeted Yield Plasmid DNA Preparation
Our premium Targeted Yield service delivers specific quantities of high quality, transfection-grade DNA that you can count on. This service is intended for stable, high-copy plasmids, and includes a free upgrade to an endo-free process with sequence confirmation, restriction digest, and sterility testing. We now offer large-scale, high-quality plasmids ranging from 250 µg to 5 grams, ideal for cell and gene therapy, drug discovery, and vaccine research.
TARGETED YIELD PLASMID DNA PREPARATION
Our premium Targeted Yield service delivers specific quantities of high quality, transfection-grade DNA that you can count on. This service is intended for stable, high-copy plasmids, and includes a free upgrade to an endo-free process with sequence confirmation, restriction digest, and sterility testing. Yield options range from 250ug to 20mg, with 100mg or larger preps available upon request.Place an Order
Service & Analysis Options
| Targeted Yield (Amount Based) | ||
|
Service Options |
Turnaround Time | Starting from 4-5 business days |
| Plasmids Accepted |
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| Spectrophotometric DNA Quality Analysis | ||
| Targeted Yield Verification | ||
| Transfection-Grade (Endotoxin Free) | ||
| DNA Normalization | ||
| Aliquots or Custom Tubes | ||
| Analysis Options | DNA Sequencing* | |
| Sequence Consensus Assembly and Analysis | ||
| Full-Service DNA Sequence Confirmation | ||
| Restriction Digest and Agarose Gel Analysis* | ||
| Sterility Testing |
= Included
= Add-on
*Includes one reaction per plasmid; added reactions available as an add-on.
Need your prep faster? Have an unstable, low copy vector? Check out our Standard-Scale (Per Run) Service.
TARGETED YIELDS AVAILABLE
| Targeted Yield Service (Amount-Based) Now offering 100 mg to 5 g yields! | |||||||
| Available Yields for High-Copy, Stable Plasmids* | 250 µg | 1 mg | 5 mg | 10 mg | 20 mg | 100 mg | 250 mg |
| 500 mg | 1 g | 500 mg | 3 g | 4 g | 5 g | ||
*Actual yield will vary with the nature of the plasmid, bacterial hosts, and other factors.
Choice of Starting Material
DNA
Transformation provided as a service*
Glycerol Stock
Provide 200 μL frozen stock
Bacterial Colony
Please provide fresh colonies (<3 days old)
*For DNA samples, GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.
Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.