FragmentGENE

FragmentGENEs are double-stranded, linear DNA fragments of 100-2,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that GENEWIZ has developed for standard gene synthesis products. We provide FragmentGENEs in a lyophilized form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.


Place an Order   
Building

FragmentGENE Limited Time Offer

Take advantage of our early access program
Pricing starting as low as $0.09/bp

Features And Benefits

High fidelity: More than 80% of the recombinant colonies from cloning FragmentGENE will contain your desired insert

Easy gene assembly: Easily assemble multiple gene fragments to create a larger construct for downstream applications

Fast delivery time: Fast turnaround time starting in just a few days to meet your research deadlines

Affordable: Very cost-effective to meet your strict budget constraints

Quality control: Stringent quality control process to ensure greater than 80% cloning efficiency

Ease of use: GENEWIZ’s Sanger Sequencing, with an extensive worldwide network of dropboxes, makes identifying the correct clones faster and more convenient

Project Turnaround Time

Sequence Length* Quantity Completion Time**
100-500 bp 500 ng 3-5 business days
501-750 bp 500 ng
750-1,000 bp 1,000 ng
1,001-1,250 bp 1,000 ng
5-8 business days
1,251-1,500 bp
1,501-1,750 bp
1,751-2,000 bp 

*Only sequences with overall GC content between 20% and 80% qualify for FragmentGENE service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time; Shipping time is expected to add 1-2 business days to completion time.

Quality Control Procedure

  • Size verification by gel electrophoresis
  • Sequence confirmation via Sanger sequencing
  • Sequence identification by mass spectrometry

Applications

  • Assemble multiple gene fragments to make full-length gene constructs for protein expression and purification
  • gRNA expression cassettes for CRISPR/Cas9-based gene editing
  • Donor constructs for gene editing experiments
  • Template for in vitro transcription

Deliverables

Standard deliverables include 500-1,000 ng of lyophilized double-stranded DNA, depending on sequence length.


HOW TO ORDER

add text here

Place your order using our online order form


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3) | Live Chat