DNA Libraries

GENEWIZ DNA Libraries offer a wide range of library types, reliable service, and best-in-class consultation from our Ph.D.-level customer support scientists. With competitive pricing and project completion in as little as 11 days, discover why hundreds of scientists from leading research facilities choose GENEWIZ for our partially (NNK) or completely randomized (NNN) nucleotide libraries.

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Synthetic DNA Library Construction

  • Combinatorial (Classical)​​

    Create a library of completely randomized (NNN) or partially randomized (NNK/NNS) mutants with multiple, different combinations of genetic elements.

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  • Trimer-Controlled (Smart)​​

    Specify an exact combination of codons, a precise percentage of desired amino acids (as low as 5%) and reduce frameshift mutations for the best mutant integrity.

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  • Variant​​

    Introduce singular/scanning site-directed mutagenesis in a user-defined 24 base pair region or truncate a sequence to characterize and optimize the desired protein features and function.

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  • sgRNA

    Accelerate CRISPR screening programs with single guide RNA (sgRNA) libraries containing knock-out/knock-in variants. Choose from pre-made or custom generated offerings.

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DNA and Variant Library Applications

  • Protein Engineering: Build and identify proteins with novel or improved stability and functional characteristics from a library of mutants
  • Enzyme Engineering: Modify an enzyme for enhanced activity for use in industrial biotechnology
  • Directed Evolution: Build a library of variants with your desired mutations for applications in evolutionary biology
  • Synthetic Biology: Build new metabolic pathways with different combinations of genetic elements for applications in metabolic engineering
  • Antibody Development: Test and identify mutant sequences with improved antigen expression
  • CRISPR/Gene Editing: Create knock-out/knock-in variants for high-throughput screening

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Featured Application: Antibody Development

The success of an in-vitro antibody discovery and development program is contingent on the diversity of functional candidates as well as the ability to enhance favorable characteristics. Choose GENEWIZ combinatorial or trimer-controlled libraries to expedite your antibody discovery and development program while keeping the cost low.

Combinatorial DNA libraries A powerful tool for determining and optimizing biomolecule function from a parent sequence. By coupling these libraries with a screening technology such as phage display, it becomes possible for researchers to both develop and test an immense number of mutant variants in a short time. Depending on the research goals, budget, and timeline, different mutagenesis techniques can be used to create variants. The below table compares three industry standard approaches.

Trimer-controlled libraries: An effective tool that significantly reduces screening efforts compared to conventional mutagenesis methods, empower researchers with the most accuracy, precision, and control as compared to other combinatorial DNA library techniques because of the extra synthetic effort devoted to restricting codon building blocks. Download the case study below to learn why researchers at a large bio-pharmaceutical company chose GENEWIZ combinatorial trimer-controlled synthesis of antibody libraries to obtain a highly accurate mutant library.

Library Stop Codons Risk of Mutation ‘AT’ Rich Bias Assigned Amino Acids Screening Speed Screening Costs
Random (NNN) 3 High Possible No Slow $$$
Partially Random (NNK/NNS) 1 High Possible No Moderate $$
Trimer-Controlled 0 Low None Yes Fastest $

DNA Libraries Features & Benefits

  • Highly reliable: Researchers at leading pharma, biotech, and academic institutions rely on GENEWIZ for their synthetic DNA library needs.
  • Improved accuracy: More precise control over library diversity allows you to overcome the inefficiencies of traditional approaches.
  • Convenience: Whether you need a high-diversity synthetic DNA library or a small rationally-designed variant library, GENEWIZ can cater to all your DNA library needs, no matter the complexity or size.
  • High diversity: Achieve library diversity of 109 to 1012 depending on the type of project deliverable.
  • Quality control: Rigorous quality checks include statistical analysis and sequencing to ensure library diversity and quality.
  • Dedicated Ph.D.-level support at every step with real-time project updates through our online system

Synthetic DNA Library Deliverables

Library Type Library Format Diversity Vector Quality Control Deliverables
Classical Combinatorial Libraries (NNN/NNK/NNS) and Trimer- Controlled (Smart libraries) Linear DNA pool Up to 1012 variants N/A Sanger sequencing of up to 96 individual transformants (default)
Additional NGS-based diversity analysis (optional) 		1-2 µg of linear dsDNA
Certificate of Analysis (COA) with quality measurements.
Classical Combinatorial Libraries (NNN/NNK/NNS) and Trimer- Controlled (Smart libraries) Cloned Pool Up to 109 variants Customer Defined Sanger sequencing of up to 96 individual transformants (default)
Additional NGS-based diversity analysis (optional)		 5-10 µg of plasmid DNA
Certificate of Analysis (COA) with quality measurements.
Large scale DNA preparation options ranging from µg to mg.
Variant Individual Clones Customer Defined Customer Defined 100% sequence accuracy guaranteed.- Restriction digest of final plasmid 2-5 µg of plasmid DNA (standard)
Certificate of Analysis (COA) with quality measurements.
Large scale DNA preparation options ranging from µg to mg.