GENEWIZ Presents...On the Bench
Here at GENEWIZ, we are bench scientists. We love to roll up our sleeves, whip out our pipettes (maybe even a repeater, if we’re feeling adventurous), and do some science. One of the problems that we frequently encounter as bench scientists is which product or kit to choose for our particular experiment. Is the fancy, expensive kit worth it? Does it work better than a cheaper counterpart? As we started asking ourselves these questions, we realized other bench scientists (like you) had many of the same questions.
In this forum, GENEWIZ R&D scientists will set up experiments in a handful of standard conditions to test different kits. While we acknowledge that one set of experiments is not anywhere near an exhaustive test, we intend these experiments to be a starting point for discussion and an invitation to share kit / product experiences between scientists. So, we invite you to glove up, get your pipettes and gel rigs ready, and join us in our product explorations … on the bench!
Have questions or comments? Email us at firstname.lastname@example.org - we would love to hear from you!
A Matter of Taq; Comparing Common PCR DNA Polymerases
Posted April 10, 2017
How does a bench scientist choose which PCR DNA Polymerase is best suited for their needs? GENEWIZ R&D scientists evaluate six commercially-available PCR DNA polymerases on the following criteria: PCR amplification of (i) a GC-rich template; (ii) a palindromic DNA sequence; (iii) nanogram quantities of human genomic DNA, as well as (iv) the effect of ethanol, (v) template switching rate, and (vi) list price cost per reaction.