FlyCut® AvrII is expressed and purified from E.coli that carries the recombinant AvrII gene. The molecular weight is 42.3 kDa, with the recognition site at C^CTAGG. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...C↓CATGG...3’
3’...GGATC↑C...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Product Contents
|
Component |
JA101-01 |
JA101-02 |
|
FlyCut® AvrII |
50 units |
2×50 units |
|
10×FlyCut® Buffer |
250 μl |
250 μl |
|
10×DNA Loading Buffer |
1 ml |
1 ml |
US
Europe (English)
Chinese
Japanese