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EasyPure® Genomic DNA Kit

EasyPure® Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from a variety of mammalian cells, tissues, E. coli and yeast. Cells and tissues are enzymatically lysed. DNA is bound to silica-based column.

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Highlights

• DNA yield up to 15 μg.                                                                                                                                                                                                        • Complete removal of contaminants and inhibitors.                                                                                                                                                      • Column based purification, no organic extraction or ethanol precipitation.

Applications

PCR, restriction enzyme digestion and Southern blot

Storage

RNase A and Proteinase K solutions at -20˚C for one year; others at room temperature for one year

Shipping Condition

RNase A and Proteinase K on dry ice (-70˚C); Others at room temperature

 

Kit Contents

Component

EE101-01 (50 rxns)

EE101-02 (200 rxns)

EE101-11 (50 rxns)

EE101-12 (200 rxns)

Lysis Buffer 2 (LB2)

6 ml

24 ml

Wash Buffer 2 (WB2)

12 ml

2 × 22 ml

Binding Buffer 2 (BB2)

28 ml

110 ml

Clean Buffer 2 (CB2)

55 ml

2 × 110 ml

Elution Buffer (EB)

25 ml

80 ml

RNase A (20 mg/ml)

1 ml (EE101-01)

4 × 1 ml (EE101-02)

0 (EE101-11)

0 (EE101-12)

Proteinase K (20 mg/ml)

1 ml

4 × 1 ml

Genomic Spin Column with Collection Tubes

50 each

200 each