METHYLATION SEQUENCING

 

DNA methylation is an epigenetic modification that alters gene expression, often as a repressor. In eukaryotes, the nucleotide cytosine is commonly methylated to form 5-methylcytosine (5mC), especially when accompanied by guanine (CpG). DNA methylation occurs in both animals and plants, playing significant roles in embryonic development, tissue regeneration, and pathogenesis.

To uncover the methylome, Azenta offers wet and dry lab solutions, including Methyl-Seq, for global whole genome and targeted 5-methylcytosine (5mC) detection.


ENZYMATIC CONVERSION & SEQUENCING

1. Protection/Enzymatic Conversion

Submit cells, tissue, or gDNA for enzymatic conversion of unprotected cytosine

2. Library Preparation

Add adapters to ssDNA fragments

3. Sequencing

Sequence 2x150 bp on Illumina® platform

4. Data Analysis

Identify 5mC sites, calculate methylation ratios, and perform differential methylation analysis

Features & Benefits


Complete sequencing solutions from extraction and enzymatic conversion to data analysis

Superior data quality: with a guarantee of ≥80% bases with ≥Q30 (>90% on average)

Dedicated Ph.D.-level support at every step including post-delivery ​

Industry-leading turnaround time as fast as 2 weeks​

METHYLATION Sequencing Overview

After protecting modified cytosines from downstream deamination, an enzymatic step deaminates cytosine but does not affect 5caC and 5ghmC. The resulting converted sequence can be used to compare treated and untreated samples, creating a snapshot of 5mC methylation patterns across the whole genome or targeted regions of interest.

How To Order


Email | Phone +49 (0)341 520 122-41