The adaptive immune system employs an immensely diverse repertoire of lymphocyte receptors to recognize and neutralize foreign agents. Analyzing the composition of B- and T-cell receptors in vivo can provide insight into diseases and aid the development of biologics and vaccines. Surface display technology can also be used as a complementary or alternative approach to discover antibodies, including both antigen-binding (Fab) and single-chain variable fragments (scFv), targeted against an antigen.
Our suite of immunogenomics services provide next generation sequencing solutions for natural and recombinant antibody populations to achieve high-throughput analysis of diverse populations. Options include physical pairing of the VH/VL or α/β chains of each complex and non-physical pairing inferred through frequency analysis. Find the optimal solution for your project below.
|Method of Pairing Chain Sequences
B-cell receptors (BCR)
T-cell receptors (TCR)
In vivo antibody
|In vitro antibody
discovery via surface
The quantity and quality of isolated cells are critical to the performance of functional assays. In this tech note, we discuss the challenges associated with PBMC isolation and offer a robust workflow for PBMC isolation and cryopreservation leading to reproducible, high-quality results.
High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery.