Oligos Retained for Sanger
GENEWIZ Oligos Retained for Sanger service eliminates the need to coordinate between vendors before sending your samples for Sanger sequencing. We synthesize, dilute, organize, and store your primers for up to a year for use with future Sanger sequencing orders. This service is designed for the bench scientist with simplicity and convenience in mind.
How are oligos used in Sanger sequencing?
Oligos are used in Sanger sequencing as primers to initiate DNA synthesis and as terminal nucleotides to terminate further DNA synthesis.
Features & Benefits
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Save time on ordering and aliquoting primers
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Includes storage at GENEWIZ for up to 6 months (Leipzig facility) / up to 3 months (Takeley facility)
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Standard, desalted oligos up to 60 nt at 25 or 50 nmol scale
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Primers ready for Sanger sequencing as fast as the next business day
Turnaround Time
| Order Placed | EU (Leipzig Lab) | |
|---|---|---|
| Before 10 AM CET | Ready for sequencing the next business day* | |
| Order Placed | UK (Takeley Lab) | |
| With sample submission | Ready on next day after sample arrival | |
*Larger orders or sequencing oligos longer than 30 bases or including wobble bases may require additional turnaround time.
Oligos Retained for Sanger will be transferred internally for GENEWIZ sequencing projects that cannot be processed in the corresponding oligo storage facility.
Please contact
sanger.europe@azenta.com
or
sanger.UK@azenta.com
for more information.
Technical Resources
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Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.