Whole Plasmid and Amplicon Sequencing Sample Submission Guidelines
Whole Plasmid Sequencing
- GENEWIZ Drop Box – Place your samples and a copy of your order form in a GENEWIZ drop box before the cut-off time.
- Alternative Logistics – If a GENEWIZ drop box is not available, please follow the standard logistics options for your region.
| European Customers | United Kingdom Customers |
|---|---|
| GENEWIZ Germany GmbH Bahnhofstrasse 86 04158 Leipzig Germany Logistics Support: Genomics.Service.Europe@azenta.com Technical Support: Sanger.Europe@azenta.com +49 (0)341 520 122-41 |
GENEWIZ UK Ltd Hope End, Takeley Essex, CM22 6TA United Kingdom Logistics Support: Genomics.Service.UK@azenta.com Technical Support: Sanger.UK@azenta.com +44 (0)1279 873837 |
Plasmid-EZ (Whole Plasmid Sequencing)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
| Plasmid DNA* Water, EB, Low TE buffer (0.1mM EDTA) |
Minimum Amount: Quantity: ≥500 ng Volume: ≥10 µL Concentration: 50 ng/µL Normalized |
A260/A280 = 1.8-2.0 | Ambient or blue ice |
Bacteria
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Colony-EZ (Whole Plasmid Sequencing from Bacteria)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
| Bacterial Colonies Bacteria* BSL1 specimens only |
1 colony per well submitted in a 96-well agar stab plate | 1 well-grown colony per well (incubated for at least 8h) | Ambient or blue ice |
| Liquid Culture Bacteria* BSL1 specimens only |
100ul of overnight-grown liquid culture (8 to 16 hours) | Samples should be visibly cloudy | Ambient or blue ice |
| Glycerol Stock Bacteria* BSL1 specimens only |
100ul of glycerol stock | Samples should be visibly cloudy | Dry ice |
Oxford Nanopore Amplicon Sequencing
Direct Shipping:
Please use an overnight delivery service such as DHL for all samples. A printout of the confirmed quote must be included with the material.
All direct shipments should be sent to:
| European Customers | United Kingdom Customers |
|---|---|
| ATTN: NGS LAB Katja Zunkel GENEWIZ Germany GmbH Bahnhofstrasse 86 Germany Logistics Support: Genomics.Service.Europe@azenta.com Technical Support: Sanger.Europe@azenta.com +49 (0)341 520 122-41 |
ATTN: NGS LAB Katja Zunkel GENEWIZ UK Ltd Hope End, Takeley Essex, CM22 6TA United Kingdom Logistics Support: Genomics.Service.UK@azenta.com Technical Support: Sanger.UK@azenta.com +44 (0)1279 873837 |
Tubes / Plates
Organize your samples in tubes or plates following the order indicated on the sample submission form.
Tubes: Prepare samples in clearly labeled and well-organized 1.5 mL flip-cap microcentrifuge tubes. Please avoid using Parafilm to seal the tubes.
Plates: For projects with 16 or more samples, prepare samples in clearly labeled, securely sealed 96-well full-skirted PCR v-bottom plates. Arrange the samples vertically by column (i.e. A1, B1, C1, etc.)
PCR-EZ (Long-Read PCR Product Sequencing)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
Unpurified and Purified PCR Products Water, EB, Low TE buffer (0.1mM EDTA) |
Minimum Amount: Quantity: ≥500 ng Volume: ≥10 µL for purified PCR products, 20 µL for un-purified PCR products Concentration: 50 ng/µL Normalized |
A260/A280 = 1.8–2.0 | Ambient or blue ice |
Amplicon-Based Next Generation Sequencing
Direct Shipping:
Please use an overnight delivery service such as DHL for all NGS samples. A printout of the confirmed quote must be included with the material.
All direct shipments should be sent to:
| European Customers | United Kingdom Customers |
|---|---|
| ATTN: NGS LAB Katja Zunkel GENEWIZ Germany GmbH Bahnhofstrasse 86 Germany Logistics Support: Genomics.Service.Europe@azenta.com Technical Support: NGS.Europe@azenta.com +49 (0)341 520 122-41 |
ATTN: NGS LAB Katja Zunkel GENEWIZ UK Ltd Hope End, Takeley Essex, CM22 6TA United Kingdom Logistics Support: Genomics.Service.UK@azenta.com Technical Support: NGS.Europe@azenta.com +44 (0)1279 873837 |
Tubes / Plates
Organize your samples in tubes or plates following the order indicated on the sample submission form.
Tubes: Prepare samples in clearly labeled and well-organized 1.5 mL flip-cap microcentrifuge tubes. Please avoid using Parafilm to seal the tubes.
Plates: For projects with 16 or more samples, prepare samples in clearly labeled, securely sealed 96-well full-skirted PCR v-bottom plates. Arrange the samples vertically by column (i.e. A1, B1, C1, etc.)
Quality control (QC) is conducted using Qubit (not Nanodrop) and TapeStation. QC results will determine the most suitable approach based on sample quantity and quality.
Illumina Amplicon Sequencing
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
Genomic DNA Double-Stranded* Water, EB, low TE buffer (0.1mM EDTA) |
Preferred Amount: Quantity: ≥250 ng Volume: 25-50 µL Concentration: 10 – 50 ng/µL Minimum Amount: Quantity: 150 ng Volume: >25 µL Concentration: ≥6 ng/µL |
A260/A280 = 1.8-2.0 DIN >5.0 RNA-free |
Dry ice |
*Purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids accepted.
PacBio Amplicon Sequencing
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
Amplicon DNA* Low TE buffer (0.1mM EDTA) |
Preferred Amount: Quantity: ≥2 µg Volume: ≥20 µL Concentration: ≥50 ng/µL Minimum Amount: Quantity: ≥1 µg Volume: ≥20 µL Concentration: ≥50 ng/µL |
A260/A280 = 1.8-2.2 A260/A230 = ≥2.0 RNA-free |
Dry ice |
*Purified and unpurified amplicons, restriction digested material, and plasmids accepted. Additionally, we can prepare an amplicon from a wide range of starting materials, including genomic DNA, cell pellets, and tissue material. Please see the Extraction Requirements section for further details.
Custom TargetGxOne™ Amplicon Sequencing
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
Genomic DNA Double-stranded Water, EB, Low TE buffer (0.1mM EDTA) |
Preferred Amount: Quantity: ≥250 ng Volume: 25-50 µL Concentration: ≥10 ng/µL Minimum Amount: Quantity: 100 ng Volume: ≥25 µL Concentration: ≥4 ng/µL |
A260/A280 = 1.8-2.0 DIN >7.0 RNA-free |
Dry ice |
Amplicon-EZ (AMP-EZ)
| Sample Type | Quantity | Quality | Shipping Temperature |
|---|---|---|---|
|
Purified PCR Products Water, EB, Low TE buffer (0.1mM EDTA) |
Minimum Amount: Quantity: ≥500 ng Volume: ≥25 µL Concentration: 20 ng/µL Normalized |
A260/A280 = 1.8-2.0 | Ambient or blue ice |